Construct: ORF TRCN0000471239
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014826.1_s317c1
- Derived from:
- ccsbBroadEn_03332
- DNA Barcode:
- GCAATTGCAGTTCTACACTCACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SIRT6 (51548)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471239
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51548 | SIRT6 | sirtuin 6 | NM_016539.4 | 100% | 100% | |
| 2 | human | 51548 | SIRT6 | sirtuin 6 | NM_001193285.3 | 92.3% | 92.3% | 529_530ins81 |
| 3 | human | 51548 | SIRT6 | sirtuin 6 | XM_024451539.1 | 86.9% | 78.2% | (many diffs) |
| 4 | human | 51548 | SIRT6 | sirtuin 6 | NM_001321059.2 | 82.8% | 82.8% | 191_192ins183 |
| 5 | human | 51548 | SIRT6 | sirtuin 6 | NM_001321058.2 | 79.7% | 79.7% | 0_1ins216 |
| 6 | human | 51548 | SIRT6 | sirtuin 6 | NM_001321061.2 | 72.1% | 72.1% | 0_1ins216;313_314ins81 |
| 7 | human | 51548 | SIRT6 | sirtuin 6 | NM_001321060.2 | 69.8% | 56.4% | 610_611ins40;744_745ins281 |
| 8 | human | 51548 | SIRT6 | sirtuin 6 | NM_001321062.2 | 61.9% | 61.9% | 0_1ins405 |
| 9 | human | 51548 | SIRT6 | sirtuin 6 | NM_001321063.2 | 52.6% | 40.1% | 191_192ins183;427_428ins40;561_562ins281 |
| 10 | human | 51548 | SIRT6 | sirtuin 6 | NM_001321064.1 | 49.5% | 37.1% | 0_1ins216;394_395ins40;528_529ins281 |
| 11 | human | 51548 | SIRT6 | sirtuin 6 | XM_024451540.1 | 49.5% | 37.1% | 0_1ins216;394_395ins40;528_529ins281 |
| 12 | mouse | 50721 | Sirt6 | sirtuin 6 | NM_181586.3 | 78.7% | 83.3% | (many diffs) |
| 13 | mouse | 50721 | Sirt6 | sirtuin 6 | NM_001163430.1 | 69.1% | 72.1% | (many diffs) |
| 14 | mouse | 50721 | Sirt6 | sirtuin 6 | XM_006513861.4 | 68% | 72% | (many diffs) |
| 15 | mouse | 50721 | Sirt6 | sirtuin 6 | XM_011243507.3 | 43% | 47.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1131
- ORF length:
- 1065
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggtgaattac gcggcggggc tgtcgccgta cgcggacaag ggcaagtgcg 121 gcctcccgga gatcttcgac cccccggagg agctggagcg gaaggtgtgg gaactggcga 181 ggctggtctg gcagtcttcc agtgtggtgt tccacacggg tgccggcatc agcactgcct 241 ctggcatccc cgacttcagg ggtccccacg gagtctggac catggaggag cgaggtctgg 301 cccccaagtt cgacaccacc tttgagagcg cgcggcccac gcagacccac atggcgctgg 361 tgcagctgga gcgcgtgggc ctcctccgct tcctggtcag ccagaacgtg gacgggctcc 421 atgtgcgctc aggcttcccc agggacaaac tggcagagct ccacgggaac atgtttgtgg 481 aagaatgtgc caagtgtaag acgcagtacg tccgagacac agtcgtgggc accatgggcc 541 tgaaggccac gggccggctc tgcaccgtgg ctaaggcaag ggggctgcga gcctgcaggg 601 gagagctgag ggacaccatc ctagactggg aggactccct gcccgaccgg gaccTGGCAC 661 TCGCCGATGA GGCCAGCAGG AACGCCGACC TGTCCATCAC GCTGGGTACA TCGCTGCAGA 721 TCCGGCCCAG CGGGAACCTG CCGCTGGCTA CCAAGCGCCG GGGAGGCCGC CTGGTCATCG 781 TCAACCTGCA GCCCACCAAG CACGACCGCC ATGCTGACCT CCGCATCCAT GGCTACGTTG 841 ACGAGGTCAT GACCCGGCTC ATGAAGCACC TGGGGCTGGA GATCCCCGCC TGGGACGGCC 901 CCCGTGTGCT GGAGAGGGCG CTGCCACCCC TGCCCCGCCC GCCCACCCCC AAGCTGGAGC 961 CCAAGGAGGA ATCTCCCACC CGGATCAACG GCTCTATCCC CGCCGGCCCC AAGCAGGAGC 1021 CCTGCGCCCA GCACAACGGC TCAGAGCCCG CCAGCCCCAA ACGGGAGCGG CCCACCAGCC 1081 CTGCCCCCCA CAGACCCCCC AAAAGGGTGA AGGCCAAGGC GGTCCCCAGC TGCCCAACTT 1141 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1201 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1261 CTTGTGGAAA GGACGAGCAA TTGCAGTTCT ACACTCACTC ACGCGTTAAG TCgacaatca 1321 acctctggat tacaaaattt gtgaaagatt