Construct: ORF TRCN0000471290
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014311.1_s317c1
- Derived from:
- ccsbBroadEn_02730
- DNA Barcode:
- CATGAGCAAGGCCTAGCCCATTGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DIP2A (23181)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471290
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23181 | DIP2A | disco interacting protein 2... | NM_001146115.2 | 100% | 100% | |
| 2 | human | 23181 | DIP2A | disco interacting protein 2... | NM_206890.3 | 94.8% | 94.8% | 655_783del |
| 3 | human | 23181 | DIP2A | disco interacting protein 2... | NM_001353944.2 | 92.8% | 91.3% | (many diffs) |
| 4 | human | 23181 | DIP2A | disco interacting protein 2... | NM_206891.3 | 90.7% | 90.1% | (many diffs) |
| 5 | human | 23181 | DIP2A | disco interacting protein 2... | XM_011529502.1 | 90.4% | 90.4% | (many diffs) |
| 6 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028305.1 | 90.4% | 90.4% | (many diffs) |
| 7 | human | 23181 | DIP2A | disco interacting protein 2... | NM_206889.3 | 89.7% | 89.7% | 655_783del;2524_2667del |
| 8 | human | 23181 | DIP2A | disco interacting protein 2... | XM_011529501.1 | 65.9% | 65.9% | 283_387del;760_891del;2632_3630del |
| 9 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028304.1 | 57.7% | 57.7% | 283_387del;760_891del;2632_4143del |
| 10 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028303.1 | 53.9% | 53.9% | 283_387del;760_891del;2632_4434del |
| 11 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028301.1 | 53.5% | 53.5% | 283_387del;760_891del;2632_4470del |
| 12 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028300.1 | 52.6% | 52.6% | 283_387del;760_891del;2632_4545del |
| 13 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028298.2 | 52.2% | 52.2% | 2395_4584del |
| 14 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028297.1 | 51% | 51% | 283_387del;2500_4689del |
| 15 | human | 23181 | DIP2A | disco interacting protein 2... | NM_001353943.1 | 50.7% | 50.7% | 655_783del;2524_4713del |
| 16 | human | 23181 | DIP2A | disco interacting protein 2... | NM_015151.4 | 50.7% | 50.7% | 655_783del;2524_4713del |
| 17 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028295.2 | 50.7% | 50.7% | 655_783del;2524_4713del |
| 18 | human | 23181 | DIP2A | disco interacting protein 2... | NM_001353942.1 | 50.7% | 50.7% | 655_786del;2527_4716del |
| 19 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028294.2 | 50.7% | 50.7% | 655_786del;2527_4716del |
| 20 | human | 23181 | DIP2A | disco interacting protein 2... | NM_001146116.1 | 50.5% | 50.5% | 655_783del;902_903insAGTTCAGCAACC;2512_4701del |
| 21 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028293.1 | 49.6% | 49.6% | 283_387del;760_888del;2629_4818del |
| 22 | human | 23181 | DIP2A | disco interacting protein 2... | XM_011529492.2 | 49.6% | 49.6% | 283_387del;760_891del;2632_4821del |
| 23 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028291.1 | 49.6% | 49.6% | 283_387del;760_891del;2632_4821del |
| 24 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028292.1 | 49.6% | 49.6% | 283_387del;760_891del;2632_4821del |
| 25 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028302.2 | 48.6% | 47.7% | (many diffs) |
| 26 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028299.1 | 47.5% | 46.7% | (many diffs) |
| 27 | mouse | 64451 | Dip2a | disco interacting protein 2... | XR_001779571.1 | 75.3% | (many diffs) | |
| 28 | mouse | 64451 | Dip2a | disco interacting protein 2... | NM_001081419.2 | 43.4% | 45.5% | (many diffs) |
| 29 | mouse | 64451 | Dip2a | disco interacting protein 2... | XM_006513939.3 | 43.2% | 45.2% | (many diffs) |
| 30 | mouse | 64451 | Dip2a | disco interacting protein 2... | XM_006513941.3 | 41.5% | 43.2% | (many diffs) |
| 31 | mouse | 64451 | Dip2a | disco interacting protein 2... | XM_006513940.3 | 41.2% | 43.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2460
- ORF length:
- 2394
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 tggccatggc tgaccgcggg tgcccgctgg aggcggcgcc gctgcctgcc gaggtgcggg 121 agagcctggc tgagctggag ctggagctgt cggaaggtga catcactcaa aaaggatatg 181 aaaagaaaag ggcaaagctg cttgcacgtt atataccgct tattcaagga atagacccat 241 ctctgcaagc agagaataga attcctgggc cctcacaaac cacggccgct gcacccaagc 301 agcagaagtc tcggcccacc gcctcgaggg atgagcgctt ccggtcagat gtccacactg 361 aagccgtgca agcagctttg gccaaataca aagagaggaa gatgcctatg ccttcgaaga 421 gacgttctgt ccttgtgcat tcgtctgtgg aaacctacac ccctccagac acgtcgtctg 481 cctcagaaga tgagggctct ttacggcgac ccgggcgact cacctccact ccgctccaga 541 gccattccag cgtcgagccc tggctcgacc gggtcattca gggctcgtcc acctcatcct 601 ctgcatcctc cacctcatct cacccgggag ggagacccac cactgctccc agtgctgcag 661 ccacgccggg ggccgccgct accactgcac tcgcaggcct cgaggcccac acccacatag 721 gtgtccctgt gaacagcaga gtgtcctcca aaatccagca gcttctgaac accctgaaga 781 ggccaaagcg ccctccactg aaggagttct ttgtggatga ttttgaggaa ttgttggaag 841 ttcagcaacc agatccaaat cagccaaagc ctgagggaag cgagacgagt gtgctgagag 901 gggagcctct cactgcaggt gtcccccgac cgccgtcgct gttggccacc ttgcagcgct 961 ggggcacaac acagcccaaa tccccctgtc tgactgcctt ggatacaact gggaaagccg 1021 tctacactct cacctatggt aaactttgga gtcggagttt aaaactagct tatactctac 1081 ttaataaact gacaagtaag aatgaacctc tacttaaacc tggagacaga gtggcgctcg 1141 tgtttccgaa tagtgaccct gtgatgttca tggttgcatt ttatgggtgt ctcctggcag 1201 agctggttcc tgtccccata gaagtgccat taacaagaaa ggatgcaggc agccagcagg 1261 ttgggtttct gctgggcagc tgtggagtct tcttggccct gaccacagac gcttgtcaga 1321 aaggcctccc caaggcacag acaggagagg tggcagcttt caaaggttgg cccccgctct 1381 cctggctagt gattgatggg aagcatctag ccaagccccc aaaggactgg caccctctgg 1441 cccaggacac agggactggg actgcctaca ttgagtataa aaccagcaaa gaaggcagta 1501 cggtgggggt cacagtgtcc cacgcatccc tgctggcaca gtgccgggct ctgacccagg 1561 cgtgcgggta ctcagaagct gaaacattaa caaacgtgct ggatttcaaa agggatgctg 1621 gtctgtggca tggcgtgtta acaagcgtca tgaacaggat gcacgtggtc agcgtcccct 1681 acgcgctgat gaaggcgaac ccactctcct ggatccagaa agtgtgcttc tataaagctc 1741 gggccgcgct ggtgaagtcg cgagacatgc actggtctct cctagctcag cggggccaga 1801 gggacgtcag cctcagctca ctgcgcatgc tgattgtggc cgatggtgcc aacccgtggt 1861 cgatctcctc ctgtgacgcc ttcctcaacg tcttccagtc cagaggtctg aggccagagg 1921 tcatctgtcc ttgtgcaagt tctcctgagg cgctgactgt cgccatccgc aggccacctg 1981 atctgggagg accacctcca agaaaagcag tcctgtcgat gaacggtcta agttatggtg 2041 ttatcagagt ggatactgaa gaaaagttgt cagtccttac tgttcaggac gttggtcagg 2101 tgatgcctgg agctaatgta tgtgttgtga agttagaagg taccccttat ctttgtaaaa 2161 ctgatgaagt gggagaaata tgcgtcagtt ccagtgcaac tggcacagcg tactatggat 2221 tgcttggaat cacgaagaat gtgtttgagg cagttccggt caccacagga ggagcaccca 2281 tctttgacag gccattcacc aggacaggcc tgctgggctt catcgggcct gacaaccTGG 2341 TCTTCATCGT GGGCAAACTG GACGGGCTGA TGGTCACTGG AGTTCGCAGA CACAATGCAG 2401 ATGACGTTGT GGCCACCGCA CTGGCCGTGG AGCCCATGAA GTTTGTCTAC AGAGGCAGGT 2461 GATGCGCTGC GGACCCCCAC GCCGGGAGTA GATTCCTTCA TTTGCAGCTC GGGAAGTCTT 2521 TGTGCAGTAG ATGGAAAGCC CAGCAAACTT GAATTCACAA AGCCTAAAAA TTGTTGTACA 2581 TCAGAAAACT CCTAAAGTAA AAGGAAAATA TTTGCAGCAA AAAAAAAAAA AAAGGGCGGC 2641 CGCTCTAGAG TATCCCTCGA GGGGCCCAAG CTTACGCGTA CCCAGCTTTC TTGTACAAAG 2701 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2761 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2821 ACGACATGAG CAAGGCCTAG CCCATTGCAC GCGTTAAGTC gacaatcaac ctctggatta 2881 caaaatttgt gaaagatt