Transcript: Mouse XM_006513939.3

PREDICTED: Mus musculus disco interacting protein 2 homolog A (Dip2a), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Dip2a (64451)
Length:
6380
CDS:
90..4808

Additional Resources:

NCBI RefSeq record:
XM_006513939.3
NBCI Gene record:
Dip2a (64451)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006513939.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000251183 TGACGGCAGCTACCGCTAAAC pLKO_005 301 CDS 100% 3.600 5.040 N Dip2a n/a
2 TRCN0000251182 AGAAGTCTCGGGCTACCAACT pLKO_005 325 CDS 100% 4.050 3.240 N Dip2a n/a
3 TRCN0000251184 CACTCAGAAAGGATATGAGAA pLKO_005 188 CDS 100% 4.950 3.465 N Dip2a n/a
4 TRCN0000258155 TCAGAAGATGAGGGCTCTTTA pLKO_005 504 CDS 100% 13.200 7.920 N Dip2a n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006513939.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488016 GCAACCCTCAGCCCTGAGCAATAA pLX_317 6.4% 86.1% 92.6% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000492097 CTATCCCCCACAGGATAACAGCCA pLX_317 8.7% 86.1% 92.6% V5 (many diffs) n/a
3 ccsbBroadEn_07848 pDONR223 100% 47.9% 49.9% None (many diffs) n/a
4 ccsbBroad304_07848 pLX_304 0% 47.9% 49.9% V5 (many diffs) n/a
5 TRCN0000472190 GGCTTTACTGGAGGTTCTATCATC pLX_317 3.7% 47.9% 49.9% V5 (many diffs) n/a
6 ccsbBroadEn_02730 pDONR223 99.4% 43.2% 45.2% None (many diffs) n/a
7 ccsbBroad304_02730 pLX_304 0% 43.2% 45.2% V5 (many diffs) n/a
8 TRCN0000471290 CATGAGCAAGGCCTAGCCCATTGC pLX_317 13.3% 43.2% 45.2% V5 (not translated due to prior stop codon) (many diffs) n/a
9 TRCN0000480527 TTCGGAAGTCTATGAAGTACAAAT pLX_317 10% 42.5% 41.1% V5 (not translated due to prior stop codon) (many diffs) n/a
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