Transcript: Human NM_001353944.2

Homo sapiens disco interacting protein 2 homolog A (DIP2A), transcript variant 10, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
DIP2A (23181)
Length:
2679
CDS:
242..2518

Additional Resources:

NCBI RefSeq record:
NM_001353944.2
NBCI Gene record:
DIP2A (23181)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001353944.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000116012 GCACGTTATATACCGCTTATT pLKO.1 380 CDS 100% 13.200 18.480 N DIP2A n/a
2 TRCN0000116015 CCATAGAAGTGCCATTAACAA pLKO.1 1272 CDS 100% 5.625 7.875 N DIP2A n/a
3 TRCN0000116014 ACGGTCTAAGTTATGGTGTTA pLKO.1 2079 CDS 100% 4.950 6.930 N DIP2A n/a
4 TRCN0000116013 CGTGTTAACAAGCGTCATGAA pLKO.1 1690 CDS 100% 4.950 6.930 N DIP2A n/a
5 TRCN0000284734 GACTGCCTACATTGAGTATAA pLKO_005 1516 CDS 100% 13.200 9.240 N DIP2A n/a
6 TRCN0000272360 TGGCGCTCGTGTTTCCGAATA pLKO_005 1188 CDS 100% 10.800 7.560 N DIP2A n/a
7 TRCN0000116016 CCTGGAGCTAATGTATGTGTT pLKO.1 2162 CDS 100% 4.950 3.465 N DIP2A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001353944.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02730 pDONR223 99.4% 92.8% 91.3% None (many diffs) n/a
2 ccsbBroad304_02730 pLX_304 0% 92.8% 91.3% V5 (many diffs) n/a
3 TRCN0000471290 CATGAGCAAGGCCTAGCCCATTGC pLX_317 13.3% 92.8% 91.3% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000480527 TTCGGAAGTCTATGAAGTACAAAT pLX_317 10% 87.3% 82.1% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroadEn_07848 pDONR223 100% 85.1% 85% None (many diffs) n/a
6 ccsbBroad304_07848 pLX_304 0% 85.1% 85% V5 (many diffs) n/a
7 TRCN0000472190 GGCTTTACTGGAGGTTCTATCATC pLX_317 3.7% 85.1% 85% V5 (many diffs) n/a
8 TRCN0000488016 GCAACCCTCAGCCCTGAGCAATAA pLX_317 6.4% 48.1% 48.1% V5 (not translated due to prior stop codon) (many diffs) n/a
9 TRCN0000492097 CTATCCCCCACAGGATAACAGCCA pLX_317 8.7% 48.1% 48.1% V5 (many diffs) n/a
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