Construct: ORF TRCN0000471348
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007777.1_s317c1
- Derived from:
- ccsbBroadEn_10777
- DNA Barcode:
- CAATAATAGTGCTCTGAGAGCACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DGUOK (1716)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471348
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318862.2 | 52.2% | 50.5% | (many diffs) |
| 2 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318863.2 | 52.2% | 50.5% | (many diffs) |
| 3 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318860.2 | 51.1% | 51.1% | 153_416del |
| 4 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318861.2 | 51.1% | 51.1% | 153_416del |
| 5 | human | 1716 | DGUOK | deoxyguanosine kinase | XM_024452739.1 | 51.1% | 51.1% | 153_416del |
| 6 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_080918.3 | 48.6% | 48.6% | 1_291del |
| 7 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318859.2 | 45.5% | 45.5% | 1_291del;423_424insAGGTCTGTGTACAGTGAC |
| 8 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134893.2 | 39.5% | 1_209del;486_698del | |
| 9 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134896.2 | 36.9% | 1_209del;341_342insAGGTCTGTGTACAGTGAC;468_680del | |
| 10 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134898.2 | 33.9% | 1_209del;362_477del;602_814del | |
| 11 | human | 1716 | DGUOK | deoxyguanosine kinase | XM_011532647.2 | 33.5% | 32.4% | (many diffs) |
| 12 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_080916.3 | 33.2% | 33.2% | 1_291del;444_707del |
| 13 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134894.2 | 32.6% | 1_209del;359_506del;634_846del | |
| 14 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134897.2 | 31% | 1_295del;445_550del;678_890del | |
| 15 | human | 1716 | DGUOK | deoxyguanosine kinase | XR_001738656.1 | 27.7% | 1_375del;528_643del;768_995del | |
| 16 | human | 1716 | DGUOK | deoxyguanosine kinase | XR_244926.3 | 26.9% | 1_374del;524_671del;799_1026del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 182
- ORF end:
- 458
- ORF length:
- 276
- Sequence:
-
1 GTGGAGACTG AAGTTAGGCC AGCTTGGCAC TTGATGTAAT TCTCCTTGGA ATTTGCCCTT 61 TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG TGGTTCAAAG TTTTTTTCTT 121 CCATTTCAGG TGTCGTGAGG CTAGCATCGA TTGATCAACA AGTTTGTACA AAAAAGTTGG 181 CATGATGTAC CGGGAGCCAG CACGATGGTC CTACACATTC CAGACATTTT CCTTTTTGAG 241 CCGCCTGAAA GTACAGCTGG AGCCCTTCCC TGAGAAACTC TTACAGGCCA GGAAGCCAGT 301 ACAGATCTTT GAGAGGTCTG TGTACAGTGA CAGGCTCCAC TTTGAGGCTC TGATGAACAT 361 TCCAGTGCTG GTGTTGGATG TCAATGATGA TTTTTCTGAG GAAGTAACCA AACAAGAAGA 421 CCTCATGAGA GAGGTAAACA CCTTTGTAAA GAATCTGTAC CCAACTTTCT TGTACAAAGT 481 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 541 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 601 CGACAATAAT AGTGCTCTGA GAGCACTACG CGTTAAGTCg acaatcaacc tctggattac 661 aaaatttgtg aaagatt