Construct: ORF TRCN0000471365
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017557.1_s317c1
- Derived from:
- ccsbBroadEn_12312
- DNA Barcode:
- TTAATTAGCCCCATTGTGCGTCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHX33 (56919)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471365
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56919 | DHX33 | DEAH-box helicase 33 | NM_001199699.2 | 90.2% | 90% | 1_154delinsA;157A>C;159A>G |
| 2 | human | 56919 | DHX33 | DEAH-box helicase 33 | NM_020162.4 | 68.1% | 68% | 1_673delinsA;676A>C;678A>G |
| 3 | human | 56919 | DHX33 | DEAH-box helicase 33 | XM_017024877.1 | 57.7% | 57.7% | 0_1ins612 |
| 4 | human | 56919 | DHX33 | DEAH-box helicase 33 | XR_934069.2 | 49.6% | (many diffs) | |
| 5 | human | 56919 | DHX33 | DEAH-box helicase 33 | XR_001752570.2 | 43.9% | (many diffs) | |
| 6 | mouse | 216877 | Dhx33 | DEAH (Asp-Glu-Ala-His) box ... | XM_011248927.2 | 86.7% | 89.6% | (many diffs) |
| 7 | mouse | 216877 | Dhx33 | DEAH (Asp-Glu-Ala-His) box ... | NM_178367.4 | 62.5% | 64.4% | (many diffs) |
| 8 | mouse | 216877 | Dhx33 | DEAH (Asp-Glu-Ala-His) box ... | XM_011248926.1 | 40.8% | 40.2% | (many diffs) |
| 9 | mouse | 216877 | Dhx33 | DEAH (Asp-Glu-Ala-His) box ... | XM_006532927.2 | 29.3% | 29.1% | (many diffs) |
| 10 | mouse | 216877 | Dhx33 | DEAH (Asp-Glu-Ala-His) box ... | XR_001779954.1 | 22.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1515
- ORF length:
- 1449
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggtgattgtg atgtcagcta cgatggatgt ggacctgttc tctcagtatt 121 tcaatggcgc ccccgtcctc tacctagagg gtcggcagca tccgatccag gtgttttaca 181 ccaaacagcc tcagaatgat tacctgcacg ccgcgcttgt ctccgtcttc cagatccacc 241 aggaagcccc ttcttcacag gacatcctgg tgttcctcac tgggcaggag gagatcgaag 301 ccatgagcaa gacgtgccga gacattgcaa agcacctccc agacggctgc cctgcgatgc 361 tggtccttcc tctgtacgcc tccctgccct atgcacagca gctccgagtc ttccaagggg 421 ccccaaaggg ctatcgcaaa gtgatcattt caaccaacat cgctgaaacc tccataacca 481 ttacaggaat aaaatatgta gttgacacgg gcatggttaa agcaaagaag tataaccctg 541 acagtggtct tgaggtgtta gcagtgcagc gggtatcgaa gacgcaggct tggcagcgca 601 cagggagggc tggcagagag gacagtggca tctgctaccg gctctacacg gaggacgagt 661 ttgagaagtt tgataagatg accgtgccag agatccagag gtgtaacctg gccagtgtga 721 tgcttcagct tctagcaatg aaagtcccaa atgtgctcac ctttgacttc atgtcgaagc 781 catctccaga tcacattcag gcggccattg cccaactgga cctgttaggt gctcttgaac 841 ataaggatga ccagcttacc ctgactccaa tgggaagaaa gatggcagca tttcctttag 901 aacccaaatt tgccaaaacc atcctcatgt cccccaaatt ccactgtaca gaggagatcc 961 tgaccattgt ctccctgctg tctgtggaca gcgtcctcca caaccctcct tcccggcgag 1021 aggaagtgca aggggTCCGC AAGAAGTTCA TATCCAGCGA GGGGGATCAC ATGACCCTGC 1081 TCAATATCTA TCGGACCTTC AAAAACCTAG GCGGAAATAA GGATTGGTGC AAAGAGAATT 1141 TTGTCAACAG CAAGAATATG ACGCTGGTAG CAGAAGTCAG AGCACAGCTG AGGGACATCT 1201 GCTTAAAGAT GTCAATGCCA ATCGCATCAT CCCGAGGAGA CGTGGAGAGT GTCCGCCGCT 1261 GCCTGGCTCA CAGCCTCTTC ATGAGCACCG CCGAGCTTCA GCCAGATGGC ACCTATGCCA 1321 CCACGGACAC CCACCAGCCA GTGGCCATCC ACCCGTCGTC TGTCCTCTTC CACTGCAAGC 1381 CGGCCTGCGT CGTGTACACT GAGCTGCTCT ACACCAACAA GTGCTACATG CGGGACCTCT 1441 GCGTCATAGA TGCACAGTGG CTGTACGAGG CTGCCCCTGA GTACTTTAGG AGGAAGCTGA 1501 GAACCGCCAG AAACTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1561 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1621 TTTCGATTTC TTGGCTTTAT AATCTTGTGG AAAGGACGAT TAATTAGCCC CATTGTGCGT 1681 CGGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att