Construct: ORF TRCN0000471367
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008037.1_s317c1
- Derived from:
- ccsbBroadEn_09915
- DNA Barcode:
- GGGACCGATTACGATATGACAAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ST6GALNAC3 (256435)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471367
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 256435 | ST6GALNAC3 | ST6 N-acetylgalactosaminide... | NM_152996.4 | 99.8% | 99.6% | 667C>A |
| 2 | human | 256435 | ST6GALNAC3 | ST6 N-acetylgalactosaminide... | NM_001349111.2 | 95.3% | 94.6% | (many diffs) |
| 3 | human | 256435 | ST6GALNAC3 | ST6 N-acetylgalactosaminide... | XM_017000937.2 | 92.8% | 92.5% | (many diffs) |
| 4 | human | 256435 | ST6GALNAC3 | ST6 N-acetylgalactosaminide... | NM_001349107.2 | 88.1% | 88.1% | 623_624ins108 |
| 5 | human | 256435 | ST6GALNAC3 | ST6 N-acetylgalactosaminide... | NM_001349108.2 | 77.5% | 76.3% | (many diffs) |
| 6 | human | 256435 | ST6GALNAC3 | ST6 N-acetylgalactosaminide... | NM_001349109.2 | 72.3% | 70.2% | (many diffs) |
| 7 | human | 256435 | ST6GALNAC3 | ST6 N-acetylgalactosaminide... | NM_001349106.2 | 71% | 70.8% | 0_1ins264;403C>A |
| 8 | human | 256435 | ST6GALNAC3 | ST6 N-acetylgalactosaminide... | XM_017000941.2 | 71% | 70.8% | 0_1ins264;403C>A |
| 9 | human | 256435 | ST6GALNAC3 | ST6 N-acetylgalactosaminide... | XM_017000942.2 | 71% | 70.8% | 0_1ins264;403C>A |
| 10 | human | 256435 | ST6GALNAC3 | ST6 N-acetylgalactosaminide... | NM_001349110.2 | 69.3% | 68.5% | (many diffs) |
| 11 | human | 256435 | ST6GALNAC3 | ST6 N-acetylgalactosaminide... | NM_001160011.3 | 68.7% | 67.8% | (many diffs) |
| 12 | human | 256435 | ST6GALNAC3 | ST6 N-acetylgalactosaminide... | NM_001349105.2 | 65.8% | 64.9% | (many diffs) |
| 13 | human | 256435 | ST6GALNAC3 | ST6 N-acetylgalactosaminide... | XM_017000938.1 | 64.1% | 63.1% | (many diffs) |
| 14 | human | 256435 | ST6GALNAC3 | ST6 N-acetylgalactosaminide... | XM_017000939.1 | 63.5% | 62.5% | (many diffs) |
| 15 | human | 256435 | ST6GALNAC3 | ST6 N-acetylgalactosaminide... | XR_001737094.2 | 60.8% | (many diffs) | |
| 16 | human | 256435 | ST6GALNAC3 | ST6 N-acetylgalactosaminide... | NR_146056.2 | 13.1% | (many diffs) | |
| 17 | mouse | 20447 | St6galnac3 | ST6 (alpha-N-acetyl-neurami... | NM_011372.2 | 83.6% | 85.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 981
- ORF length:
- 915
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctgcatcctg aagagaaagt ctgtgattgc tgtgagcttc atagcagcgt 121 tccttttcct gctggttgtg cgtcttgtaa atgaagtgaa tttcccattg ctactaaact 181 gctttggaca acctggtaca aagtggatac cattctccta cacatacagg cggccccttc 241 gaactcacta tggatacata aatgtgaaga cacaagagcc tttgcaactg gactgtgacc 301 tttgtgccat agtgtcaaac tcaggtcaga tggttggcca gaaggtggga aatgagatag 361 atcgatcctc ctgcatttgg agaatgaaca atgcccccac caaaggttat gaagaagatg 421 tcggccgcat gaccatgatt cgagttgtgt cccataccag cgttcctctt ttgctaaaaa 481 accctgatta ttttttcaag gaagcgaata ctactattta tgttatttgg ggacctttcc 541 gcaatatgag gaaagatggc aatggcatcg tttaCAACAT GTTGAAAAAG ACAGTTGGTA 601 TCTATCCGAA TGCCCAAATA TACGTGACCA CAGAGAAGCG CATGAGTTAC TGTGATGGAG 661 TTTTTAAGAA GGAAACTGGG AAGGACAGAG TCCAGTCTGG CTCATATCTC AGCACAGGGT 721 GGTTTACCTT CATTCTGGCC ATGGACGCCT GTTATGGCAT TCACGTCTAC GGGATGATAA 781 ATGACACCTA CTGCAAGACA GAAGGGTATA GAAAAGTCCC CTACCATTAT TATGAACAAG 841 GAAGAGATGA GTGTGATGAA TATTTTCTTC ATGAACATGC CCCATATGGG GGTCATAGGT 901 TTATCACTGA AAAGAAAGTG TTTGCTAAAT GGGCCAAGAA GCACAGGATA ATATTTACAC 961 ATCCAAACTG GACATTGTCT TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1021 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1081 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGGA CCGATTACGA 1141 TATGACAAGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt