Construct: ORF TRCN0000471391
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016476.1_s317c1
- Derived from:
- ccsbBroadEn_12249
- DNA Barcode:
- TAAAAACCGCAAATACATCTTTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IWS1 (55677)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471391
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XM_017004468.2 | 8.4% | 1.9% | 1_736del;893_1836del |
| 2 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XM_011511450.1 | 6.9% | 1.6% | 1_1372del;1529_2229del |
| 3 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_001738828.1 | 6.6% | 1_1574del;1731_2329del | |
| 4 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XM_017004467.2 | 6.3% | 1.4% | 1_1357del;1514_2454del |
| 5 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | NM_017969.3 | 6.3% | 1.4% | 1_1357del;1514_2457del |
| 6 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XM_006712626.3 | 6.3% | 1.4% | 1_1372del;1529_2469del |
| 7 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XM_005263707.3 | 6.3% | 1.4% | 1_1372del;1529_2472del |
| 8 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_001738827.1 | 5.5% | 1_1574del;1731_2795del | |
| 9 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_001738829.2 | 5.4% | 1_1638del;1795_2859del | |
| 10 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_922960.2 | 5.3% | 1_1574del;1731_2927del | |
| 11 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_001738826.2 | 5.2% | 1_1638del;1795_2991del | |
| 12 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_001738832.1 | 5.1% | (many diffs) | |
| 13 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_001738830.1 | 4.9% | (many diffs) | |
| 14 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_001738831.1 | 4.9% | (many diffs) | |
| 15 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_001738833.2 | 4.8% | (many diffs) | |
| 16 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_001738834.2 | 4.8% | (many diffs) | |
| 17 | mouse | 73473 | Iws1 | IWS1, SUPT6 interacting pro... | XM_006526291.3 | 6.1% | 1.6% | (many diffs) |
| 18 | mouse | 73473 | Iws1 | IWS1, SUPT6 interacting pro... | XM_006526292.3 | 6.1% | 1.6% | (many diffs) |
| 19 | mouse | 73473 | Iws1 | IWS1, SUPT6 interacting pro... | NM_173441.3 | 6.1% | 1.6% | (many diffs) |
| 20 | mouse | 73473 | Iws1 | IWS1, SUPT6 interacting pro... | XR_386017.2 | 1.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 320
- ORF end:
- 476
- ORF length:
- 156
- Sequence:
-
1 TCAGCCGTCG CTTCATGTGA CTCCACGGAG TACCGGGCGC CGTCCAGGCA CCTCGATTAG 61 TTCTCGAGCT TTTGGAGTAC GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA TGCGATGGAG 121 TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC 181 TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG 241 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG 301 TTTGTACAAA AAAGTTGGCA TGAAGAGAAG GATCTGTTTG GGAGTGACAG TGAGTCAGGC 361 AATGAAGAAG AAAATCTTAT TGCAGACATA TTTGGAGAAT CTGGTGATGA AGAGGAAGAA 421 GAATTTACAG GTTTTAACCA AGAAGATCTG GAAGAAGAAA AAGGTGAAAC ACAGGTACCC 481 AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT 541 CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA 601 TATATCTTGT GGAAAGGACG ATAAAAACCG CAAATACATC TTTGCACGCG TTAAGTCgac 661 aatcaacctc tggattacaa aatttgtgaa agatt