Construct: ORF TRCN0000471417
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015082.1_s317c1
- Derived from:
- ccsbBroadEn_15529
- DNA Barcode:
- TATTTTCACACTCGAGTTCGACGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIN4 (5303)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471417
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5303 | PIN4 | peptidylprolyl cis/trans is... | NM_006223.4 | 99.7% | 100% | 381C>T |
| 2 | human | 5303 | PIN4 | peptidylprolyl cis/trans is... | NM_001170747.1 | 61.2% | 50.8% | (many diffs) |
| 3 | human | 5303 | PIN4 | peptidylprolyl cis/trans is... | NR_033187.2 | 25.6% | (many diffs) | |
| 4 | human | 728758 | PIN4P1 | peptidylprolyl cis/trans is... | NR_003571.1 | 16.1% | (many diffs) | |
| 5 | mouse | 69713 | Pin4 | protein (peptidyl-prolyl ci... | NM_027181.1 | 94.6% | 96.9% | (many diffs) |
| 6 | mouse | 105247253 | LOC105247253 | peptidyl-prolyl cis-trans i... | XM_011250928.1 | 94.4% | 96.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 459
- ORF length:
- 393
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 TTGGCATGCC GCCCAAAGGA AAAAGTGGTT CTGGAAAAGC GGGGAAAGGG GGAGCAGCCT 121 CTGGGAGTGA CAGTGCTGAC AAGAAGGCTC AAGGTCCCAA AGGTGGTGGC AATGCAGTAA 181 AGGTCAGACA CATTCTATGT GAAAAACATG GCAAAATCAT GGAAGCCATG GAAAAGTTAA 241 AGTCTGGGAT GAGATTCAAT GAAGTGGCCG CACAGTATAG TGAAGATAAA GCCAGGCAAG 301 GGGGTGACTT GGGTTGGATG ACCAGAGGGT CCATGGTGGG ACCATTTCAA GAAGCAGCAT 361 TTGCCTTGCC TGTAAGTGGG ATGGATAAGC CTGTGTTTAC AGACCCACCG GTTAAGACAA 421 AATTTGGATA TCATATTATT ATGGTTGAAG GAAGAAAATA CCCAACTTTC TTGTACAAAG 481 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 541 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 601 ACGATATTTT CACACTCGAG TTCGACGAAC GCGTTAAGTC gacaatcaac ctctggatta 661 caaaatttgt gaaagatt