Construct: ORF TRCN0000471418
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017541.1_s317c1
- Derived from:
- ccsbBroadEn_01263
- DNA Barcode:
- CGAGTTAATCTGGCAATCTATCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP2R1A (5518)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471418
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5518 | PPP2R1A | protein phosphatase 2 scaff... | NM_014225.6 | 100% | 100% | |
| 2 | human | 5518 | PPP2R1A | protein phosphatase 2 scaff... | NM_001363656.2 | 69.6% | 69.6% | 0_1ins537 |
| 3 | human | 5518 | PPP2R1A | protein phosphatase 2 scaff... | NR_033500.2 | 31.1% | 1_45del;213_214ins101;1712_5251del | |
| 4 | mouse | 51792 | Ppp2r1a | protein phosphatase 2, regu... | NM_016891.3 | 90.5% | 99.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1833
- ORF length:
- 1767
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggccgac ggcgacgact cgctgtaccc catcgcggtg ctcatagacg 121 aactccgcaa tgaggacgtt cagcttcgcc tcaacagcat caagaagctg tccaccatcg 181 ccttggccct tggggttgaa aggacccgaa gtgagcttct gcctttcctt acagatacca 241 tctatgatga agatgaggtc ctcctggccc tggcagaaca gctgggaacc ttcactaccc 301 tggtgggagg cccagagtac gtgcactgcc tgctgccacc gctggagtcg ctggccacag 361 tggaggagac agtggtgcgg gacaaggcag tggagtcctt acgggccatc tcacacgagc 421 actcgccctc tgacctggag gcgcactttg tgccgctagt gaagcggctg gcgggcggcg 481 actggttcac ctcccgcacc tcggcctgcg gcctcttctc cgtctgctac ccccgagtgt 541 ccagtgctgt gaaggcggaa cttcgacagt acttccggaa cctgtgctca gatgacaccc 601 ccatggtgcg gcgggccgca gcctccaagc tgggggagtt tgccaaggtg ctggagctgg 661 acaacgtcaa gagtgagatc atccccatgt tctccaacct ggcctctgac gagcaggact 721 cggtgcggct gctggcggtg gaggcgtgcg tgaacatcgc ccagcttctg ccccaggagg 781 atctggaggc cctggtgatg cccactctgc gccaggccgc tgaagacaag tcctggcgcg 841 tccgctacat ggtggctgac aagttcacag agctccagaa agcagtgggg cctgagatca 901 ccaagacaga cctggtccct gccttccaga acctgatgaa agactgtgag gccgaggtga 961 gggccgcagc ctcccacaag gtcaaagagt tctgtgaaaa cctctcagct gactgtcggg 1021 agaatgtgat catgtcccag atcttgccct gcatcaagga gctggtgtcc gatgccaacc 1081 aacatgtcaa gtctgccctg gcctcagtca tcatgggtct ctctcccatc ttgggcaaag 1141 acaacaccat cgagcacctc ttgcccctct tcctggctca gctgaaggat gagtgccctg 1201 aggtacggct gaacatcatc tctaacctgg actgtgtgaa cgaggtgatt ggcatccggc 1261 agctgtccca gtccctgctc cctgccattg tggagctggc tgaggacgcc aagtggcggg 1321 tgcggctggc catcattgag tacatgcccc tcctGGCTGG ACAGCTGGGA GTGGAGTTCT 1381 TTGATGAGAA ACTTAACTCC TTGTGCATGG CCTGGCTTGT GGATCATGTA TATGCCATCC 1441 GCGAGGCAGC CACCAGCAAC CTGAAGAAGC TAGTGGAAAA GTTTGGGAAG GAGTGGGCCC 1501 ATGCCACAAT CATCCCCAAG GTCTTGGCCA TGTCCGGAGA CCCCAACTAC CTGCACCGCA 1561 TGACTACGCT CTTCTGCATC AATGTGCTGT CTGAGGTCTG TGGGCAGGAC ATCACCACCA 1621 AGCACATGCT ACCCACGGTT CTGCGCATGG CTGGGGACCC GGTTGCCAAT GTCCGCTTCA 1681 ATGTGGCCAA GTCTCTGCAG AAGATAGGGC CCATCCTGGA CAACAGCACC TTGCAGAGTG 1741 AAGTCAAGCC CATCCTAGAG AAGCTGACCC AGGACCAGGA TGTGGACGTC AAATACTTTG 1801 CCCAGGAGGC TCTGACTGTT CTGTCTCTCG CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1861 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1921 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG 1981 AGTTAATCTG GCAATCTATC TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 2041 ttgtgaaaga tt