Construct: ORF TRCN0000471677
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009734.1_s317c1
- Derived from:
- ccsbBroadEn_07884
- DNA Barcode:
- TCTGCTGTATCACGGGGTGGGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RYBP (23429)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471677
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23429 | RYBP | RING1 and YY1 binding protein | NM_012234.6 | 99.8% | 100% | 189A>G |
| 2 | mouse | 56353 | Rybp | RING1 and YY1 binding protein | NM_019743.3 | 93.5% | 99.1% | (many diffs) |
| 3 | mouse | 628746 | Rybp-ps | RING1 and YY1 binding prote... | XR_873606.2 | 37% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 750
- ORF length:
- 684
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac catgggcgac aagaagagcc cgaccaggcc aaaaagacaa gcgaaacctg 121 ccgcagacga agggttttgg gattgtagcg tctgcacctt cagaaacagt gctgaagcct 181 ttaaatgcag catctgcgat gtgaggaaag gcacctccac cagaaaacct cggatcaatt 241 ctcagctggt ggcgcaacaa gtggcacaac agtatgccac cccaccaccc cctaaaaagg 301 agaagaagga gaaagttgaa aagcaggaca aagagaaacc TGAGAAAGAC AAGGAAATTA 361 GTCCTAGTGT TACCAAGAAA AATACCAACA AGAAAACCAA ACCAAAGTCT GACATTCTGA 421 AAGATCCTCC TAGTGAAGCA AACAGCATAC AGTCTGCAAA TGCTACAACA AAGACCAGCG 481 AAACAAATCA CACCTCAAGG CCCCGGCTGA AAAACGTGGA CAGGAGCACT GCACAGCAGT 541 TGGCAGTAAC TGTGGGCAAC GTCACCGTCA TTATCACAGA CTTTAAGGAA AAGACTCGCT 601 CCTCATCGAC ATCCTCATCC ACAGTGACCT CCAGTGCAGG GTCAGAACAG CAGAACCAGA 661 GCAGCTCGGG GTCAGAGAGC ACAGACAAGG GCTCCTCCCG TTCCTCCACG CCAAAGGGCG 721 ACATGTCAGC AGTCAATGAT GAATCTTTCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 781 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 841 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCTGC 901 TGTATCACGG GGTGGGTTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 961 tgaaagatt