Construct: ORF TRCN0000471707
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008862.1_s317c1
- Derived from:
- ccsbBroadEn_08486
- DNA Barcode:
- AATGTGCACGACCTAGGGATGGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUDT11 (55190)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471707
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55190 | NUDT11 | nudix hydrolase 11 | NM_018159.4 | 99.5% | 98.7% | 9C>G;260A>G |
| 2 | human | 170685 | NUDT10 | nudix hydrolase 10 | NM_001304963.2 | 98.5% | 98.1% | (many diffs) |
| 3 | human | 170685 | NUDT10 | nudix hydrolase 10 | NM_153183.3 | 98.5% | 98.1% | (many diffs) |
| 4 | mouse | 58242 | Nudt11 | nudix (nucleoside diphospha... | NM_021431.2 | 87.6% | 94.5% | (many diffs) |
| 5 | mouse | 102954 | Nudt10 | nudix (nucleoside diphospha... | NM_001031664.1 | 87.3% | 94.5% | (many diffs) |
| 6 | mouse | 102954 | Nudt10 | nudix (nucleoside diphospha... | XM_006527546.2 | 87.3% | 94.5% | (many diffs) |
| 7 | mouse | 71207 | Nudt4 | nudix (nucleoside diphospha... | XM_006514116.2 | 73.5% | 82.7% | (many diffs) |
| 8 | mouse | 71207 | Nudt4 | nudix (nucleoside diphospha... | NM_027722.4 | 73.1% | 82.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 558
- ORF length:
- 492
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gtggaaaccc aaccagacgc ggacctacga ccccgagggg ttcaagaagc 121 gggcggcgtg cctgtgcttc cggagcgaac gcgaggacga ggtcctgtta gtgagtagca 181 gccggtaccc ggaccgctgg atcgtgccgg gcgggggcat ggagcccgag gaggagccgg 241 gcggtgcggc ggtccgagag gtgtacgaag aagcgggagt caagggGAAG TTAGGCCGGC 301 TCCTGGGCGT CTTCGAACAG AACCGGGATC GCAAGCACAG AACGTACGTG TATGTACTGA 361 CTGTCACGGA GCTGCTGGAG GATTGGGAAG ATTCGGTTAG CATTGGGAGG AAGCGAGAGT 421 GGTTCAAAGT CGAAGATGCC ATCAAGGTTC TCCAGTGCCA CAAGCCCGTG CACGCCGAAT 481 ATCTGGAGAA ACTAAAGCTG GGCGGTTCCC CAACCAATGG AAACTCCATG GCCCCATCCT 541 CGCCAGATAG CGATCCCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAATGTGC ACGACCTAGG 721 GATGGTGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt