Construct: ORF TRCN0000471716
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012514.1_s317c1
- Derived from:
- ccsbBroadEn_07187
- DNA Barcode:
- TTGTCGAATTCGCCTCTGGCGCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471716
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7919 | DDX39B | DExD-box helicase 39B | NM_004640.7 | 99.9% | 99.7% | 886T>C |
| 2 | human | 7919 | DDX39B | DExD-box helicase 39B | NM_080598.6 | 99.9% | 99.7% | 886T>C |
| 3 | human | 10212 | DDX39A | DExD-box helicase 39A | NM_005804.4 | 78.5% | 89.7% | (many diffs) |
| 4 | human | 10212 | DDX39A | DExD-box helicase 39A | XM_011527620.1 | 78.5% | 89.7% | (many diffs) |
| 5 | human | 7919 | DDX39B | DExD-box helicase 39B | NR_037852.2 | 63.1% | (many diffs) | |
| 6 | human | 100532737 | ATP6V1G2-DDX39B | ATP6V1G2-DDX39B readthrough... | NR_037853.1 | 55.6% | 1_803del;1689T>C;2088_2305del | |
| 7 | human | 10212 | DDX39A | DExD-box helicase 39A | XM_006722606.4 | 42% | 48.3% | (many diffs) |
| 8 | human | 10212 | DDX39A | DExD-box helicase 39A | XM_011527621.3 | 42% | 48.3% | (many diffs) |
| 9 | human | 10212 | DDX39A | DExD-box helicase 39A | XM_024451311.1 | 42% | 48.3% | (many diffs) |
| 10 | human | 10212 | DDX39A | DExD-box helicase 39A | XM_024451312.1 | 42% | 48.3% | (many diffs) |
| 11 | mouse | 53817 | Ddx39b | DEAD (Asp-Glu-Ala-Asp) box ... | NM_001252457.1 | 92% | 99.2% | (many diffs) |
| 12 | mouse | 53817 | Ddx39b | DEAD (Asp-Glu-Ala-Asp) box ... | NM_019693.3 | 92% | 99.2% | (many diffs) |
| 13 | mouse | 68278 | Ddx39 | DEAD (Asp-Glu-Ala-Asp) box ... | NM_197982.3 | 80% | 90.4% | (many diffs) |
| 14 | mouse | 68278 | Ddx39 | DEAD (Asp-Glu-Ala-Asp) box ... | XM_006531332.3 | 80% | 90.4% | (many diffs) |
| 15 | mouse | 68278 | Ddx39 | DEAD (Asp-Glu-Ala-Asp) box ... | XM_017312949.1 | 74.8% | 84.3% | (many diffs) |
| 16 | mouse | 68278 | Ddx39 | DEAD (Asp-Glu-Ala-Asp) box ... | XM_017312950.1 | 74.8% | 84.3% | (many diffs) |
| 17 | mouse | 68278 | Ddx39 | DEAD (Asp-Glu-Ala-Asp) box ... | XM_017312952.1 | 43.4% | 48.5% | (many diffs) |
| 18 | mouse | 68278 | Ddx39 | DEAD (Asp-Glu-Ala-Asp) box ... | XM_017312951.1 | 40.5% | 45.3% | (many diffs) |
| 19 | mouse | 102465886 | Mir8094 | microRNA 8094 | NR_106169.1 | 7.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1350
- ORF length:
- 1284
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agagaacgat gtggacaatg agctcttgga ctatgaagat gatgaggtgg 121 agacagcagc tgggggagat ggggctgagg cccctgccaa gaaggatgtc aagggctcct 181 atgtctccat ccacagctct ggctttcgtg acttcctgct caagccagag ttgctccggg 241 ccattgtcga ctgtggcttt gagcatccgt cagaagtcca gcatgagtgc atccctcagg 301 ccattctggg aatggatgtc ctgtgccagg ccaagtcggg catgggaaag acagcagtgt 361 ttgtcttggc cacactgcaa cagctggagc cagttactgg gcaggtgtct gtactggtga 421 tgtgtcacac tcgggagttg gcttttcaga tcagcaagga atatgagcgc ttctctaaat 481 acatgcccaa tgtcaaggtt gctgtttttt ttggtggtct gtctatcaag aaggatgaag 541 aggtgctgaa gaagaactgc ccgcatatcg tcgtggggac tccaggccgt atcctagccc 601 tggctcgaaa taagagcctc aacctcaaac acattaaaca ctttattttg gatgaatgtg 661 ataagatgct tgaacagctc gacatgcgtc gggatgtcca ggaaattttt cgcatgaccc 721 cccacgagaa gcaggtcatg atgttcagtg ctaccttgag caaagagatc cgtccagtct 781 gccgcaagtt catgcaagat ccaatggaga tcttcgtgga tgatgagacg aagttgacgc 841 tgcatgggtt gcagcagtac tacgtgaaac tgaaggacaa cgagaagaac cggaagctct 901 ttgaccttct ggatgtcctt gagttcaacc aggtggtgat ctttgtgaag ccTGTGCAGC 961 GGTGCATTGC CTTGGCCCAG CTACTAGTGG AGCAGAACTT CCCAGCCATT GCCATCCACC 1021 GTGGGATGCC CCAGGAGGAG AGGCTTTCTC GGTATCAGCA GTTTAAAGAT TTTCAACGAC 1081 GAATTCTTGT GGCTACCAAC CTATTTGGCC GAGGCATGGA CATCGAGCGG GTGAACATTG 1141 CTTTTAATTA TGACATGCCT GAGGATTCTG ACACCTACCT GCATCGGGTG GCCAGAGCAG 1201 GCCGGTTTGG CACCAAGGGC TTGGCTATCA CATTTGTGTC CGATGAGAAT GATGCCAAGA 1261 TCCTCAATGA TGTGCAGGAT CGCTTTGAGG TCAATATTAG TGAGCTGCCT GATGAGATAG 1321 ACATCTCCTC CTACATTGAA CAGACACGGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1381 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1441 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTGTC 1501 GAATTCGCCT CTGGCGCAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1561 tgaaagatt