Construct: ORF TRCN0000471738
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014988.3_s317c1
- Derived from:
- ccsbBroadEn_08892
- DNA Barcode:
- TACAGAGCCGCCAGAGATGGGTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NADK (65220)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471738
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65220 | NADK | NAD kinase | NM_001198993.2 | 99.9% | 99.7% | 786C>A |
| 2 | human | 65220 | NADK | NAD kinase | NM_001353641.2 | 99.9% | 99.7% | 786C>A |
| 3 | human | 65220 | NADK | NAD kinase | NM_023018.5 | 99.9% | 99.7% | 786C>A |
| 4 | human | 65220 | NADK | NAD kinase | XM_017002143.2 | 87.3% | 80.6% | 786C>A;1099_1100ins83;1170_1171ins85 |
| 5 | human | 65220 | NADK | NAD kinase | NM_001198995.1 | 87.2% | 82.3% | (many diffs) |
| 6 | human | 65220 | NADK | NAD kinase | XM_006710839.2 | 81% | 80.7% | 264_575del;1098C>A |
| 7 | human | 65220 | NADK | NAD kinase | NM_001353642.2 | 80.4% | 80.2% | 0_1ins261;525C>A |
| 8 | human | 65220 | NADK | NAD kinase | XM_011542006.1 | 76.8% | 63.2% | 0_1ins203;188_189ins106;477C>A |
| 9 | human | 65220 | NADK | NAD kinase | XM_011542007.2 | 76.8% | 63.2% | 0_1ins203;188_189ins106;477C>A |
| 10 | human | 65220 | NADK | NAD kinase | XM_011542008.1 | 76.8% | 63.2% | 0_1ins203;188_189ins106;477C>A |
| 11 | human | 65220 | NADK | NAD kinase | XM_011542009.2 | 76.8% | 63.2% | 0_1ins203;188_189ins106;477C>A |
| 12 | human | 65220 | NADK | NAD kinase | NM_001198994.2 | 75.4% | 75.1% | 264_575del;704_826del;1221C>A |
| 13 | human | 65220 | NADK | NAD kinase | XM_017002144.2 | 74.8% | 74.6% | 0_1ins336;450C>A |
| 14 | human | 65220 | NADK | NAD kinase | XM_017002145.2 | 72.1% | 70.8% | (many diffs) |
| 15 | human | 65220 | NADK | NAD kinase | XM_017002146.1 | 72.1% | 70.8% | (many diffs) |
| 16 | human | 65220 | NADK | NAD kinase | XR_946747.2 | 60.8% | (many diffs) | |
| 17 | human | 65220 | NADK | NAD kinase | XR_001737371.2 | 58.6% | (many diffs) | |
| 18 | mouse | 192185 | Nadk | NAD kinase | NM_001159637.1 | 84.2% | 90.8% | (many diffs) |
| 19 | mouse | 192185 | Nadk | NAD kinase | NM_138671.2 | 84.2% | 90.8% | (many diffs) |
| 20 | mouse | 192185 | Nadk | NAD kinase | XM_006538662.3 | 84.2% | 90.8% | (many diffs) |
| 21 | mouse | 192185 | Nadk | NAD kinase | XM_006538663.3 | 84.2% | 90.8% | (many diffs) |
| 22 | mouse | 192185 | Nadk | NAD kinase | XM_006538664.3 | 67.6% | 73.1% | (many diffs) |
| 23 | mouse | 192185 | Nadk | NAD kinase | XM_006538665.3 | 61.3% | 64.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1404
- ORF length:
- 1338
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aatggaacaa gaaaaaatga ccatgaataa ggaattgagt ccagacgcgg 121 ctgcttactg ctgctcggcc tgccacggcg atgagacctg gagttacaac caccccatcc 181 ggggccgggc caagtctcgc agcctgtctg cctcgcccgc cctggggagc accaaggagt 241 tcaggaggac acgctctctt catgggccat gcccggtgac cacttttgga ccaaaggcct 301 gtgtgctgca gaacccccag accatcatgc acattcagga ccccgcgagc cagcggctga 361 cgtggaacaa gtccccaaag agcgtccttg tcatcaagaa gatgagagat gccagcctac 421 tgcagccgtt caaggagctc tgcacgcacc tcatggagga gaacatgatc gtgtatgtgg 481 aaaagaaagt gctagaagac cctgccatcg ccagcgatga aagctttggg gcagtgaaga 541 agaaattctg tacctttcga gaagattatg atgacatttc caatcagata gacttcatca 601 tctgcctggg gggagacggg acgctgctgt acgcttcctc gcttttccag ggcagcgtcc 661 ctccggtcat ggccttccac ctgggctccc tgggcttcct gaccccattc agctttgaga 721 actttcagtc ccaagttact caggtgatag aggggaacgc agctgttgtt ctccggagtc 781 ggctgaaggt cagggtggtg aaggagctcc gggggaagaa gacggccgtg cacaatgggc 841 tgggtgagaa aggctcgcag gctgcaggcc tggacatgga tgtcgggaag caggccatgc 901 agtaccaggt cctgaatgag gtggtgattg acagaggccc ctcctcctac ctgtccaatg 961 tggatgtcta cctggacgga cacctcatca ccacggtgca gggcgacgga gtgatcgtgt 1021 ccaccccgac gggcagcacg gcgtatgcgg ccgcggccgg ggcctccatg atccacccca 1081 acgtgccggc catcatgatc acgcccatct gcccccactc gctgtccttc cggcccatcg 1141 tggtccccgc aggggtcgag ctgaagatca tgctgtcacc tgaagcaagg aacacagcat 1201 gggtgtcctt tgatggacgg aagagacaag agatccgcca tggagacagc atcagcatca 1261 ctacctcatg ctacccgctc ccctccatct gtgtgcggga ccccgtgagc gactggtttg 1321 agagcctcgc ccagtgcctg cattggaacg tccggaagaa gcaagcccac ttcgaggagg 1381 aggaGGAGGA GGAGGAGGAG GGCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1441 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1501 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT ACAGAGCCGC 1561 CAGAGATGGG TGGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1621 att