Construct: ORF TRCN0000471782
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013485.1_s317c1
- Derived from:
- ccsbBroadEn_06260
- DNA Barcode:
- CTATGGAAGTGACGCACGTAGGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GATA3 (2625)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471782
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2625 | GATA3 | GATA binding protein 3 | NM_001002295.2 | 99.9% | 99.7% | 8T>C |
| 2 | human | 2625 | GATA3 | GATA binding protein 3 | XM_005252442.2 | 99.9% | 99.7% | 8T>C |
| 3 | human | 2625 | GATA3 | GATA binding protein 3 | XM_005252443.5 | 99.9% | 99.7% | 8T>C |
| 4 | human | 2625 | GATA3 | GATA binding protein 3 | NM_002051.3 | 99.6% | 99.5% | 8T>C;776_777insAGA |
| 5 | mouse | 14462 | Gata3 | GATA binding protein 3 | NM_008091.3 | 89.6% | 96.1% | (many diffs) |
| 6 | mouse | 14462 | Gata3 | GATA binding protein 3 | XM_006497354.1 | 89.6% | 96.1% | (many diffs) |
| 7 | mouse | 14462 | Gata3 | GATA binding protein 3 | XM_006497355.3 | 89.4% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1398
- ORF length:
- 1332
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggaatgga ggcgacggcg gaccagccgc gctgggtgag ccaccaccac cccgccgtgc 121 tcaacgggca gcacccggac acgcaccacc cgggcctcag ccactcctac atggacgcgg 181 cgcagtaccc gctgccggag gaggtggatg tgctttttaa catcgacggt caaggcaacc 241 acgtcccgcc ctactacgga aactcggtca gggccacggt gcagaggtac cctccgaccc 301 accacgggag ccaggtgtgc cgcccgcctc tgcttcatgg atccctaccc tggctggacg 361 gcggcaaagc cctgggcagc caccacaccg cctccccctg gaatctcagc cccttctcca 421 agacgtccat ccaccacggc tccccggggc ccctctccgt ctaccccccg gcctcgtcct 481 cctccttgtc ggggggccac gccagcccgc acctcttcac cttcccgccc accccgccga 541 aggacgtctc cccggaccca tcgctgtcca ccccaggctc ggccggctcg gcccggcagg 601 acgagaaaga gtgcctcaag taccaggtgc ccctgcccga cagcatgaag ctggagtcgt 661 cccactcccg tggcagcatg accgccctgg gtggagcctc ctcgtcgacc caccacccca 721 tcaccaccta cccgccctac gtgcccgagt acagctccgg actcttcccc cccagcagcc 781 tgctgggcgg ctcccccacc ggcttcggat gcaagtccag gcccaaggcc cggtccagca 841 cagaaggcag ggagtgtgtg aactgtgggg caacctcgac cccactgtgg cggcgagatg 901 gcacgggaca ctacctgtgc aacgccTGCG GGCTCTATCA CAAAATGAAC GGACAGAACC 961 GGCCCCTCAT TAAGCCCAAG CGAAGGCTGT CTGCAGCCAG GAGAGCAGGG ACGTCCTGTG 1021 CGAACTGTCA GACCACCACA ACCACACTCT GGAGGAGGAA TGCCAATGGG GACCCTGTCT 1081 GCAATGCCTG TGGGCTCTAC TACAAGCTTC ACAATATTAA CAGACCCCTG ACTATGAAGA 1141 AGGAAGGCAT CCAGACCAGA AACCGAAAAA TGTCTAGCAA ATCCAAAAAG TGCAAAAAAG 1201 TGCATGACTC ACTGGAGGAC TTCCCCAAGA ACAGCTCGTT TAACCCGGCC GCCCTCTCCA 1261 GACACATGTC CTCCCTGAGC CACATCTCGC CCTTCAGCCA CTCCAGCCAC ATGCTGACCA 1321 CGCCCACGCC GATGCACCCG CCATCCAGCC TGTCCTTTGG ACCACACCAC CCCTCCAGCA 1381 TGGTCACCGC CATGGGTTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1441 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1501 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTATGGA AGTGACGCAC 1561 GTAGGCTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt