Construct: ORF TRCN0000471786
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012814.2_s317c1
- Derived from:
- ccsbBroadEn_14614
- DNA Barcode:
- AACATGAGCGCGCTTACCGAGTAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DGUOK (1716)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471786
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_080916.3 | 100% | 100% | |
| 2 | human | 1716 | DGUOK | deoxyguanosine kinase | XM_011532647.2 | 97.8% | 97.8% | 423_424insAGGTCTGTGTACAGTGAC |
| 3 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_080918.3 | 68.2% | 68.2% | 443_444ins264 |
| 4 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318859.2 | 66% | 66% | 425_426ins282 |
| 5 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318860.2 | 64.9% | 64.9% | 0_1ins291 |
| 6 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318861.2 | 64.9% | 64.9% | 0_1ins291 |
| 7 | human | 1716 | DGUOK | deoxyguanosine kinase | XM_024452739.1 | 64.9% | 64.9% | 0_1ins291 |
| 8 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318862.2 | 62.8% | 62.8% | 0_1ins291;132_133insAGGTCTGTGTACAGTGAC |
| 9 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318863.2 | 62.8% | 62.8% | 0_1ins291;132_133insAGGTCTGTGTACAGTGAC |
| 10 | human | 1716 | DGUOK | deoxyguanosine kinase | XR_244926.3 | 62.6% | 1_83del;674_675ins116;799_1026del | |
| 11 | human | 1716 | DGUOK | deoxyguanosine kinase | XR_001738656.1 | 59.7% | 1_84del;524_525ins148;768_995del | |
| 12 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134897.2 | 56.9% | (many diffs) | |
| 13 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134894.2 | 56% | (many diffs) | |
| 14 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134898.2 | 53% | (many diffs) | |
| 15 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134893.2 | 42.2% | (many diffs) | |
| 16 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134896.2 | 40.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 897
- ORF length:
- 831
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgcgggccgc ctctttctaa gtcggcttcg agcacccttc agttccatgg 121 ccaagagccc actcgagggc gtttcctcct ccagaggcct gcacgcgggg cgcgggcccc 181 gaaggctctc catcgaaggc aacattgctg tgggaaagtc cacgtttgtg aagttactca 241 cgaaaactta cccagaatgg cacgtagcta cagaacctgt agcaacatgg cagaatatcc 301 aggctgctgg cacccaaaaa gcctgcactg cccaaagtct tggaaacttg ctggatatga 361 tgtaccggga gccagcacga tggtcctaca cattccagac attttccttt ttgagccgcc 421 tgaaagtaca gctggagccc ttccctgaga aactcttaca ggCCAGGAAG CCAGTACAGA 481 TCTTTGAGAG GTCTGTGTAC AGTGACAGGT ATATCTTTGC AAAGAATCTT TTTGAAAATG 541 GTTCCCTCAG TGACATCGAG TGGCATATCT ATCAGGACTG GCATTCTTTT CTCCTGTGGG 601 AGTTTGCCAG CCGGATCACA TTACATGGCT TCATCTACCT CCAGGCTTCT CCCCAGGTTT 661 GTTTGAAGAG ACTGTACCAG AGGGCCAGGG AGGAGGAGAA AGGAATTGAG CTGGCCTATC 721 TAGAGCAGCT GCATGGCCAA CACGAAGCCT GGCTTATTCA CAAGACAACG AAGCTCCACT 781 TTGAGGCTCT GATGAACATT CCAGTGCTGG TGTTGGATGT CAATGATGAT TTTTCTGAGG 841 AAGTAACCAA ACAAGAAGAC CTCATGAGAG AGGTAAACAC CTTTGTAAAG AATCTGTACC 901 CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT 961 CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT 1021 TATATATCTT GTGGAAAGGA CGAAACATGA GCGCGCTTAC CGAGTAAACG CGTTAAGTCg 1081 acaatcaacc tctggattac aaaatttgtg aaagatt