Construct: ORF TRCN0000471794
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016880.1_s317c1
- Derived from:
- ccsbBroadEn_09379
- DNA Barcode:
- CGTGTGGTGATATAGAGAAACATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT71 (112802)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471794
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112802 | KRT71 | keratin 71 | NM_033448.3 | 99.8% | 99.6% | 1135G>A;1391T>G;1524A>G |
| 2 | human | 112802 | KRT71 | keratin 71 | XM_017018749.1 | 84.1% | 83.9% | (many diffs) |
| 3 | human | 319101 | KRT73 | keratin 73 | NM_175068.3 | 81.8% | 81.3% | (many diffs) |
| 4 | human | 319101 | KRT73 | keratin 73 | XM_011538257.2 | 77.5% | 77% | (many diffs) |
| 5 | human | 319101 | KRT73 | keratin 73 | XR_001748677.1 | 39.6% | (many diffs) | |
| 6 | mouse | 56735 | Krt71 | keratin 71 | NM_019956.1 | 87.2% | 91.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1638
- ORF length:
- 1569
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagccgccaa ttcacctgca agtcgggagc tgccgccaag gggggcttca 121 gtggctgctc agctgtgctc tcagggggca gctcatcctc cttccgggca gggagcaaag 181 ggctcagtgg gggctttggc agccggagcc tctacagcct ggggggtgtc cggagcctca 241 atgtggccag tggcagcggg aagagtggag gctatggatt tggccggggc cgggccagtg 301 gctttgctgg aagcatgttt ggcagtgtgg ccctggggcc tgtgtgccca actgtatgcc 361 cacctggagg catccaccag gttaccgtca atgagagcct cctggccccc ctcaacgtgg 421 agctggaccc cgagatccag aaagtgcgtg cccaggagcg agagcagatc aaggctctga 481 acaacaagtt cgcctccttc atcgacaagg tgcggttcct ggagcagcag aaccaggtac 541 tggagaccaa gtgggagctg ctgcagcagc tggacctgaa caactgcaag aacaacctgg 601 agcccatcct cgagggctac atcagcaacc tgcggaagca gctggagacg ctgtctgggg 661 acagggtgag gctggactcg gagctgagga atgtgcggga cgtagtggag gactacaaga 721 agaggtatga ggaggaaatc aacaagcgga cagcagcaga gaacgagttt gtgctgctca 781 agaaggatgt ggatgctgct tacgccaata aggtggaact gcaggccaag gtggaatcca 841 tggaccagga gatcaagttc ttcaggtgtc tctttgaagc cgagatcact cagatccagt 901 cccacatcag tgacatgtct gtcatcctgt ccatggacaa caaccggaac ctagacctgg 961 acagcatcat tgacgaagtc cgcacccagt atgaggagat tgccttgaag agtaaggccg 1021 aggctgaggc cctgtaccag accaagttcc aagagcttca gctggcagct ggcaggcatg 1081 gggacgacct caaaaacacc aagaatgaaa tctcggagct cactcggctc atccagagaa 1141 tccgctcaga gatcgagaac gtgaagaagc aggcttccaa cctggagaca gccatcgctg 1201 atactgagca gcggggagac aacgcccTGA AGGATGCCCG GGCCAAGCTG GACGAGCTGG 1261 AGGGCGCCCT GCACCAGGCC AAGGAGGAGC TGGCGCGGAT GCTGCGCGAG TACCAGGAGC 1321 TCATGAGCCT GAAGCTGGCC CTGGACATGG AGATCGCCAC CTATCGCAAG CTACTGGAGA 1381 GCGAGGAGTG CAGGATGTCA GGAGAATTTC CCTCCCCTGT CAGCATCTCC ATCATCAGCA 1441 GCACCAGTGG CGGCAGTGGC TATGGCTTCC GGCCCAGCAT GGTCAGCGGT GGCTATGTGG 1501 CCAACAGCAG CAACTGCATC TCTGGAGTGT GCAGCGTGAG AGGCGGGGAG GGCAGGAGCC 1561 GGGGCAGTGC CAACGATTAC AAAGACACCC TGGGGAAGGG TTCCAGCCTG AGTGCACCCT 1621 CCAAGAAAAC CAGTCGGTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1681 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1741 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACGTGTGG TGATATAGAG 1801 AAACATCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt