Construct: ORF TRCN0000471803
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013116.1_s317c1
- Derived from:
- ccsbBroadEn_09228
- DNA Barcode:
- GTGCACCCAGGGCCGTGACTGGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM25 (84866)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471803
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84866 | TMEM25 | transmembrane protein 25 | NM_001144034.1 | 99.8% | 99.6% | 893A>G |
| 2 | human | 84866 | TMEM25 | transmembrane protein 25 | NM_001144035.1 | 99.8% | 99.6% | 893A>G |
| 3 | human | 84866 | TMEM25 | transmembrane protein 25 | XM_024448724.1 | 99.8% | 99.6% | 893A>G |
| 4 | human | 84866 | TMEM25 | transmembrane protein 25 | XM_017018430.2 | 99.5% | 99.3% | 803_804insAGC;890A>G |
| 5 | human | 84866 | TMEM25 | transmembrane protein 25 | NM_001144038.1 | 91.2% | 90% | (many diffs) |
| 6 | human | 84866 | TMEM25 | transmembrane protein 25 | XM_011543038.3 | 91.1% | 85.9% | (many diffs) |
| 7 | human | 84866 | TMEM25 | transmembrane protein 25 | XM_017018432.2 | 89.9% | 89.7% | 67_68ins96;797A>G |
| 8 | human | 84866 | TMEM25 | transmembrane protein 25 | XM_017018433.2 | 89.9% | 89.7% | 67_68ins96;797A>G |
| 9 | human | 84866 | TMEM25 | transmembrane protein 25 | NM_032780.4 | 87.8% | 87.7% | 672_803del;1025A>G |
| 10 | human | 84866 | TMEM25 | transmembrane protein 25 | XM_017018422.2 | 87.8% | 87.7% | 672_803del;1025A>G |
| 11 | human | 84866 | TMEM25 | transmembrane protein 25 | XM_005271703.2 | 81% | 76.5% | (many diffs) |
| 12 | human | 84866 | TMEM25 | transmembrane protein 25 | XM_006718926.3 | 81% | 76.5% | (many diffs) |
| 13 | human | 84866 | TMEM25 | transmembrane protein 25 | NM_001318755.1 | 80.7% | 76.3% | (many diffs) |
| 14 | human | 84866 | TMEM25 | transmembrane protein 25 | NM_001144037.1 | 80.7% | 79.6% | (many diffs) |
| 15 | human | 84866 | TMEM25 | transmembrane protein 25 | NM_001318757.1 | 79.1% | 71.4% | (many diffs) |
| 16 | human | 84866 | TMEM25 | transmembrane protein 25 | NM_001144036.1 | 67.5% | 67% | 70_71ins312;581A>G |
| 17 | human | 84866 | TMEM25 | transmembrane protein 25 | NR_134852.1 | 30.5% | (many diffs) | |
| 18 | mouse | 71687 | Tmem25 | transmembrane protein 25 | NM_027865.2 | 76.6% | 79.7% | (many diffs) |
| 19 | mouse | 71687 | Tmem25 | transmembrane protein 25 | XM_006510609.3 | 76.6% | 79.7% | (many diffs) |
| 20 | mouse | 71687 | Tmem25 | transmembrane protein 25 | XM_006510610.3 | 76.6% | 79.7% | (many diffs) |
| 21 | mouse | 71687 | Tmem25 | transmembrane protein 25 | XM_006510608.2 | 71.9% | 74.8% | (many diffs) |
| 22 | mouse | 71687 | Tmem25 | transmembrane protein 25 | XM_011242601.2 | 52.4% | 50.6% | (many diffs) |
| 23 | mouse | 71687 | Tmem25 | transmembrane protein 25 | XM_011242602.1 | 52.4% | 50.6% | (many diffs) |
| 24 | mouse | 71687 | Tmem25 | transmembrane protein 25 | XM_011242603.1 | 52.4% | 50.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1032
- ORF length:
- 966
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gctgcctcca ggcccagccg ccctccggca cacactgctg ctcctgccag 121 cccttctgag ctcaggttgg ggggagttgg agccacaaat agatggtcag acctgggctg 181 agcgggcact tcgggagaat gaacgccacg ccttcacctg ccgggtggca ggggggcctg 241 gcacccccag attggcctgg tatctggatg gacagctgca ggaggccagc acctcaagac 301 tgctgagcgt gggaggggag gccttctctg gaggcaccag caccttcact gtcactgccc 361 atcgggccca gcatgagctc aactgctctc tgcaggaccc cagaagtggc cgatcagcca 421 acgcctctgt catccttaat gtgcaattca agccagagat tgcccaagtc ggcgccaagt 481 accaggaagc tcagggccca ggcctcctgg ttgtcctgtt tgccctggtg cgtgccaacc 541 cgccggccaa tgtcacctgg atcgaccagg atgggccagt gactgtcaac acctctgact 601 tcctggtgct ggatgcgcag aactacccct ggctcaccaa ccacacggtg cagctgcagc 661 tccgcagccT GGCACACAAC CTCTCGGTGG TGGCCACCAA TGACGTGGGT GTCACCAGTG 721 CGTCGCTTCC AGCCCCAGGC CCCTCCCGGC ACCCATCTCT GATATCAAGT GACTCCAACA 781 ACCTAAAACT CAACAACGTG CGCCTGCCAC GGGAGAACAT GTCCCTCCCG TCCAACCTTC 841 AGCTCAATGA CCTCACTCCA GATTCCAGAG CAGTGAAACC AGCAGACCGG CAGATGGCTC 901 AGAACAACAG CCGGCCAGAG CTTCTGGACC CGGAGCCCGG CGGCCTCCTC ACCAGCCGAG 961 GTTTCATCCG CCTCCCAGTG CTGGGCTATA TCTATCGAGT GTCCAGCGTG AGCAGTGATG 1021 AGATCTGGCT CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1081 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1141 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTG CACCCAGGGC CGTGACTGGA 1201 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t