Construct: ORF TRCN0000471881
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010506.1_s317c1
- Derived from:
- ccsbBroadEn_01217
- DNA Barcode:
- CTAGCATCATGTCTTCGTAACTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLGLB2 (5342)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471881
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5343 | PLGLB1 | plasminogen like B1 | NM_001032392.2 | 100% | 100% | |
| 2 | human | 5342 | PLGLB2 | plasminogen like B2 | NM_002665.4 | 100% | 100% | |
| 3 | human | 5340 | PLG | plasminogen | NM_001168338.1 | 68.6% | 67.6% | (many diffs) |
| 4 | human | 285189 | PLGLA | plasminogen like A (pseudog... | NR_003506.3 | 33.4% | (many diffs) | |
| 5 | human | 5340 | PLG | plasminogen | NM_000301.3 | 11.5% | 11.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 226
- ORF end:
- 514
- ORF length:
- 288
- Sequence:
-
1 GGGGGGAGGG GTTTTATGCG ATGGAGTTTC CCCACACTGA GTGGGTGGAG ACTGAAGTTA 61 GGCCAGCTTG GCACTTGATG TAATTCTCCT TGGAATTTGC CCTTTTTGAG TTTGGATCTT 121 GGTTCATTCT CAAGCCTCAG ACAGTGGTTC AAAGTTTTTT TCTTCCATTT CAGGTGTCGT 181 GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG TTGGCATGGA ACATAAGGAA 241 GTGGTTCTTC TACTTCTTTT ATTTCTGAAA TCAGGTCAAG GAGAGCCCCT GGATGACTAT 301 GTGAATACCC AGGGGCCTTC ACTGTTCAGT GTCACTAAGA AGCAGCTGGG GGCAGGAAGC 361 AGAGAAGAAT GTGCAGCAAA ATGTGAAGAG GACAAAGAAT TCACCTGCAG GGCATTCCAA 421 TATCACAGTA AAGAGCAACA ATGTGTGATA ATGGCTGAAA ACAGGAAGTC CTCCATAATC 481 ATTAGGATGA GAGATGCAGT TTTATTTGAA AAGTACCCAA CTTTCTTGTA CAAAGTGGTT 541 GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT 601 AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC 661 TAGCATCATG TCTTCGTAAC TGCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa 721 tttgtgaaag att