Construct: ORF TRCN0000471910
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003440.1_s317c1
- Derived from:
- ccsbBroadEn_08349
- DNA Barcode:
- TACCTATGTTCTCCAGCTTGTACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STX18 (53407)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471910
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53407 | STX18 | syntaxin 18 | NM_016930.4 | 99.9% | 100% | 505C>T |
| 2 | human | 53407 | STX18 | syntaxin 18 | NM_001346281.2 | 89.3% | 69.9% | 498_550del;558C>T;754_755ins59 |
| 3 | human | 53407 | STX18 | syntaxin 18 | NM_001346282.2 | 75.7% | 75.8% | 0_1ins243;262C>T |
| 4 | human | 53407 | STX18 | syntaxin 18 | NM_001346300.1 | 75.7% | 75.8% | 0_1ins243;262C>T |
| 5 | human | 53407 | STX18 | syntaxin 18 | NR_144414.2 | 43.7% | (many diffs) | |
| 6 | mouse | 71116 | Stx18 | syntaxin 18 | NM_026959.3 | 88.5% | 92.5% | (many diffs) |
| 7 | mouse | 71116 | Stx18 | syntaxin 18 | NM_001289536.1 | 81.4% | 85% | (many diffs) |
| 8 | mouse | 71116 | Stx18 | syntaxin 18 | NM_001289535.1 | 80.6% | 83.2% | (many diffs) |
| 9 | mouse | 71116 | Stx18 | syntaxin 18 | XM_006504111.3 | 74.1% | 71.3% | (many diffs) |
| 10 | mouse | 71116 | Stx18 | syntaxin 18 | XM_006504112.3 | 66.6% | 68.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1071
- ORF length:
- 1005
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggtggacatc acgctgctat tccgggccag cgtcaagacc gtgaagacgc 121 ggaacaaggc gctgggagtg gcggtgggcg gcggggtcga tggcagccgg gacgagctgt 181 tccgccggag cccccggccc aagggcgact tctccagccg ggcccgcgaa gtgatttctc 241 acattggcaa actgagagat tttcttctgg aacacaggaa agattatatt aatgcttata 301 gccataccat gtctgaatat gggaggatga cagacacaga acgagaccag atagaccagg 361 atgcccagat attcatgagg acctgttcag aagcaattca gcaactacga acagaagctc 421 acaaggagat acattcccag caagtgaagg agcacaggac cgctgttttg gatttcattg 481 aagattactt gaaaagagta tgtaaacttt actcagaaca gagagccatc cgagttaaaa 541 gagtggtgga taagaaaaga ttatctaagt tggaaccaga accaaataca aagacaagag 601 aatccacatc ttctgagaaa gtttcacaga gtccttcaaa agactctgaa gaaaaccctg 661 ccactgaaga acgtccagaa aaaattttgg ctgaaacaca acctgaattG GGAACGTGGG 721 GAGATGGCAA AGGCGAAGAT GAGTTATCCC CAGAAGAAAT ACAAATGTTT GAACAGGAAA 781 ATCAGCGACT AATTGGTGAA ATGAACAGCT TGTTTGATGA AGTGAGGCAA ATCGAAGGGA 841 GAGTGGTTGA GATTTCCAGA CTCCAAGAGA TATTCACGGA AAAGGTTTTG CAACAGGAAG 901 CTGAGATTGA CAGCATTCAC CAGTTAGTTG TGGGGGCAAC TGAAAATATC AAGGAAGGCA 961 ACGAAGACAT AAGAGAGGCC ATTAAAAACA ACGCTGGCTT CCGCGTGTGG ATCCTCTTCT 1021 TCCTCGTGAT GTGCTCCTTC TCCTTGCTCT TCCTCGACTG GTACGACAGC TACCCAACTT 1081 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1141 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1201 CTTGTGGAAA GGACGATACC TATGTTCTCC AGCTTGTACC ACGCGTTAAG TCgacaatca 1261 acctctggat tacaaaattt gtgaaagatt