Transcript: Mouse NM_001289535.1

Mus musculus syntaxin 18 (Stx18), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Stx18 (71116)
Length:
2427
CDS:
103..1029

Additional Resources:

NCBI RefSeq record:
NM_001289535.1
NBCI Gene record:
Stx18 (71116)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001289535.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000100567 GCGACTCATTGGTGAAATGAA pLKO.1 819 CDS 100% 0.563 0.788 N Stx18 n/a
2 TRCN0000337664 GAGCACAGGAAGGAGTATATT pLKO_005 307 CDS 100% 15.000 12.000 N Stx18 n/a
3 TRCN0000100566 CGTGAAGTGATTTCTCACATT pLKO.1 262 CDS 100% 4.950 3.960 N Stx18 n/a
4 TRCN0000382438 ACCTCTCAGGACCTGGAAATA pLKO_005 1146 3UTR 100% 13.200 9.240 N Stx18 n/a
5 TRCN0000337733 ATTTCGTTGACGATTACTTAA pLKO_005 509 CDS 100% 13.200 9.240 N Stx18 n/a
6 TRCN0000379454 CATTGGGAAGCTGAGAGATTT pLKO_005 279 CDS 100% 13.200 9.240 N Stx18 n/a
7 TRCN0000100569 CGAAGGGAAAGTCGTTGAAAT pLKO.1 867 CDS 100% 13.200 9.240 N Stx18 n/a
8 TRCN0000350945 GACAGTGAAGACGCGGAATAA pLKO_005 144 CDS 100% 13.200 9.240 N Stx18 n/a
9 TRCN0000380541 GACCAGGATGCTCAGATATTC pLKO_005 391 CDS 100% 13.200 9.240 N Stx18 n/a
10 TRCN0000337732 CAGGCCTCCTATCTCCATTAC pLKO_005 1273 3UTR 100% 10.800 7.560 N Stx18 n/a
11 TRCN0000100565 GACTGAGCAGAGCAAGGAAAT pLKO.1 1250 3UTR 100% 10.800 7.560 N Stx18 n/a
12 TRCN0000376941 GCCACACCATGTCTGACTATG pLKO_005 338 CDS 100% 10.800 7.560 N Stx18 n/a
13 TRCN0000380650 TCGACAGCATTCACCAGTTAG pLKO_005 941 CDS 100% 10.800 7.560 N Stx18 n/a
14 TRCN0000376940 ACGTGCCAGCATGTGTCTCAT pLKO_005 1062 3UTR 100% 4.950 3.465 N Stx18 n/a
15 TRCN0000100568 CATTGGTGAAATGAACAGCTT pLKO.1 825 CDS 100% 2.640 1.848 N Stx18 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001289535.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08349 pDONR223 100% 80.6% 83.2% None (many diffs) n/a
2 ccsbBroad304_08349 pLX_304 0% 80.6% 83.2% V5 (many diffs) n/a
3 TRCN0000471910 TACCTATGTTCTCCAGCTTGTACC pLX_317 36.2% 80.6% 83.2% V5 (many diffs) n/a
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