Construct: ORF TRCN0000471935
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014255.1_s317c1
- Derived from:
- ccsbBroadEn_00591
- DNA Barcode:
- TTCCGACCATTCAACCTACGAGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FRG1 (2483)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471935
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2483 | FRG1 | FSHD region gene 1 | NM_004477.3 | 100% | 100% | |
| 2 | human | 100289097 | FRG1CP | FSHD region gene 1 family m... | NR_132315.1 | 86.8% | (many diffs) | |
| 3 | human | 102723780 | FRG1FP | FSHD region gene 1 family m... | NR_132320.1 | 65.5% | (many diffs) | |
| 4 | human | 102723316 | FRG1DP | FSHD region gene 1 family m... | NR_132316.1 | 65% | (many diffs) | |
| 5 | human | 284802 | FRG1BP | FSHD region gene 1 family m... | NR_003579.2 | 63.4% | (many diffs) | |
| 6 | human | 2483 | FRG1 | FSHD region gene 1 | XM_017007958.1 | 50.3% | 45.3% | 0_1ins211;48_49ins173 |
| 7 | human | 102724813 | LOC102724813 | protein FRG1B | XM_011546192.2 | 39% | 36% | (many diffs) |
| 8 | human | 102724813 | LOC102724813 | protein FRG1B | XM_011546196.3 | 39% | 37.2% | (many diffs) |
| 9 | human | 102724813 | LOC102724813 | protein FRG1B | XM_011546191.3 | 38.7% | 35.7% | (many diffs) |
| 10 | human | 102724813 | LOC102724813 | protein FRG1B | XM_011546190.3 | 35.1% | 32.1% | (many diffs) |
| 11 | human | 102724813 | LOC102724813 | protein FRG1B | XM_017030110.1 | 35.1% | 32.1% | (many diffs) |
| 12 | human | 642236 | FRG1JP | FSHD region gene 1 family m... | NR_033907.2 | 33.2% | (many diffs) | |
| 13 | human | 284802 | FRG1BP | FSHD region gene 1 family m... | NR_145491.1 | 20.2% | (many diffs) | |
| 14 | human | 283788 | LOC283788 | FSHD region gene 1 pseudogene | NR_027436.2 | 15% | (many diffs) | |
| 15 | human | 2483 | FRG1 | FSHD region gene 1 | XR_002959721.1 | 14.1% | (many diffs) | |
| 16 | mouse | 14300 | Frg1 | FSHD region gene 1 | NM_013522.3 | 90.1% | 97.2% | (many diffs) |
| 17 | mouse | 14300 | Frg1 | FSHD region gene 1 | XM_017312570.1 | 90.1% | 97.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 840
- ORF length:
- 774
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgagtactcc tacgtgaagt ctaccaagct cgtgctcaag ggaaccaaga 121 cgaagagtaa gaagaaaaag agcaaagata agaaaagaaa aagagaagaa gatgaagaaa 181 cccagcttga tattgttgga atctggtgga cagtaacaaa ctttggtgaa atttcaggaa 241 ccatagccat tgaaatggat aagggaacct atatacatgc actcgacaat ggtcttttta 301 ccctgggagc tccacacaaa gaagttgatg agggccctag tcctccagag cagtttacgg 361 ctgtcaaatt atctgattcc agaatcgccc tgaagtctgg ctatggaaaa tatcttggta 421 taaattcaga tggacttgtt gttgggcgtt cagatgcaat tggaccaaga gaacaatggg 481 aaccagtctt tcaaaatggg aaaatggctt tgttggcctc aaatagctgc tttattagat 541 gcaatgaagc aggggacata gaagcaaaaa gtaaaacagc aggagaagaa gaaatgatca 601 agattagatc ctgtgctgaa agagaaacca agaaaaaaga tgacattcca gaagaagaca 661 aaggaaatgt aaaacaatgt gaaatcaatt atgtaaagaa atttcagagc ttccaagacc 721 acaaacttaa aataagtaaa gaagacagta aaattcttaa aaaggctcgg aaagatggat 781 ttttgcaTGA GACGCTTCTG GACAGGAGAG CCAAATTGAA AGCCGACAGA TACTGCAAGT 841 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 901 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 961 TTTATATATC TTATGGAAAG GACGATTCCG ACCATTCAAC CTACGAGGAA CGCGTTAAGT 1021 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt