Construct: ORF TRCN0000471984
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002226.1_s317c1
- Derived from:
- ccsbBroadEn_15949
- DNA Barcode:
- GACGATGCTGCTGGTGATGGACGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF471 (57573)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471984
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55338 | LINC01949 | long intergenic non-protein... | NR_130914.1 | 9.6% | (many diffs) | |
| 2 | human | 100131257 | LOC100131257 | zinc finger protein 655 pse... | NR_034022.1 | 1.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 366
- ORF length:
- 300
- Sequence:
-
1 tcttcCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGAG GAGAAACCAG TGCAAAAAGC CTGAAAATTC CAAAAACCAG AATGCCTCTT 121 CTCCTCCAAA GGATCACAAC TCCTTGCCGG CAAGGGAACA AAACTGGATA GAGAATGAGT 181 TTGACAAATT GACAGAAGTA GGCTTCAGAA GGTGGGAAAT AACAAACTCC TCTGAGCTAA 241 AGGAGCATGT TCTAACCCAA TGCAAGGAAG CTAAGAACCT TGAAAAAAGG TTAGAGGAAT 301 TGCTAACTAG AATAACCAGT TTAGAGAAGA ACATAAATGA CCTGATGGAG CTGAAAAACA 361 CAGCATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 421 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 481 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGACGATGCT GCTGGTGATG GACGCACGCG 541 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt