Construct: ORF TRCN0000472011
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010650.1_s317c1
- Derived from:
- ccsbBroadEn_10823
- DNA Barcode:
- CTGCAACTGTTAATACCTCTGATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FLOT2 (2319)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472011
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2319 | FLOT2 | flotillin 2 | XM_017024394.1 | 100% | 100% | |
| 2 | human | 2319 | FLOT2 | flotillin 2 | XM_017024395.1 | 100% | 100% | |
| 3 | human | 2319 | FLOT2 | flotillin 2 | XM_017024396.1 | 97.2% | 97.8% | (many diffs) |
| 4 | human | 2319 | FLOT2 | flotillin 2 | XM_005257953.2 | 92.6% | 92.6% | 0_1ins84 |
| 5 | human | 2319 | FLOT2 | flotillin 2 | XM_024450667.1 | 92.6% | 92.6% | 0_1ins84 |
| 6 | human | 2319 | FLOT2 | flotillin 2 | NM_004475.3 | 88.5% | 88.5% | 1_147del |
| 7 | human | 2319 | FLOT2 | flotillin 2 | NM_001330170.2 | 86.1% | 86.6% | (many diffs) |
| 8 | human | 2319 | FLOT2 | flotillin 2 | XM_024450666.1 | 82.2% | 82.2% | 1_147del;579_677del |
| 9 | mouse | 14252 | Flot2 | flotillin 2 | NM_001284228.1 | 90.4% | 99.2% | (many diffs) |
| 10 | mouse | 14252 | Flot2 | flotillin 2 | NM_008028.2 | 90.4% | 99.2% | (many diffs) |
| 11 | mouse | 14252 | Flot2 | flotillin 2 | XM_006532192.2 | 90.4% | 99.2% | (many diffs) |
| 12 | mouse | 14252 | Flot2 | flotillin 2 | XM_006532193.3 | 90.4% | 99.2% | (many diffs) |
| 13 | mouse | 14252 | Flot2 | flotillin 2 | XM_006532194.1 | 83.1% | 91.8% | (many diffs) |
| 14 | mouse | 14252 | Flot2 | flotillin 2 | NM_001040403.1 | 80% | 87.8% | (many diffs) |
| 15 | mouse | 14252 | Flot2 | flotillin 2 | NM_001284227.1 | 75.1% | 82.4% | (many diffs) |
| 16 | mouse | 14252 | Flot2 | flotillin 2 | XM_006532191.3 | 67.5% | 74.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1203
- ORF length:
- 1137
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac gttgcagccc cgctgcgagg acgtagagac ggccgagggg gtagctttaa 121 ctgtgacggg tgtcgcccag gtgaagatca tgacggagaa ggaactcctg gccgtggctt 181 gtgagcagtt tctgggtaag aatgtgcagg acatcaaaaa cgtcgtcctg cagaccctgg 241 agggacatct gcgctccatc ctcgggaccc tgacagtgga gcagatttat caggaccggg 301 accagtttgc caagctggtg cgggaggtgg cagcccctga tgttggccgc atgggcattg 361 agatcctcag cttcaccatc aaggacgtgt atgacaaagt ggactatctg agctccctgg 421 gcaagacgca gactgccgtg gtgcagagag atgctgacat tggcgtggcc gaggctgaac 481 gggacgcagg catccgggaa gctgagtgca agaaggagat gctggatgtg aagttcatgg 541 cagacaccaa gattgctgac tctaagcgag ccttcgagct gcaaaagtca gccttcagtg 601 aggaggttaa catcaagaca gctgaggccc agttggccta tgagctgcag ggggcccgtg 661 aacagcagaa gatccggcag gaagagattg agattgaggt tgtgcagcgc aagaaacaga 721 ttgccgtgga ggcacaggag atcctgcgta cggacaagga gctcatcgct acagtgcgcc 781 ggcctgccga ggccgaggcc caccgcatcc agcagattgc cgagggtgaa aaggtgaagc 841 aggtcctctt ggcacaggca gaggctgaga agatccgcaa aatcggggag gcggaagcgg 901 cagtcatcga ggcgatgggc aaggcagagg ctgagcggat gaagctcaag gcagaagcct 961 accagaaata cggggatgca gccaagatgg ccttggtgct agaggccctg ccccagattg 1021 ctgccaaaat cgctgcccca cttaccaagg tcgatgagat tgtggtcctC AGTGGAGACA 1081 ACAGTAAGGT CACATCAGAA GTGAACCGAC TGCTGGCCGA GCTGCCTGCC TCTGTGCATG 1141 CCCTCACAGG CGTGGACCTG TCTAAGATAC CCCTGATCAA GAAGGCCACT GGTGTGCAGG 1201 TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1261 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1321 GGCTTTATAT ATCTTGTGGA AAGGACGACT GCAACTGTTA ATACCTCTGA TAACGCGTTA 1381 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt