Construct: ORF TRCN0000472051
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000935.1_s317c1
- Derived from:
- ccsbBroadEn_00751
- DNA Barcode:
- TCGTGCCCACAAAACATGTAAAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HMGB2 (3148)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472051
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3148 | HMGB2 | high mobility group box 2 | NM_001130688.1 | 100% | 100% | |
| 2 | human | 3148 | HMGB2 | high mobility group box 2 | NM_001130689.1 | 100% | 100% | |
| 3 | human | 3148 | HMGB2 | high mobility group box 2 | NM_002129.4 | 100% | 100% | |
| 4 | mouse | 97165 | Hmgb2 | high mobility group box 2 | NM_008252.3 | 89.8% | 96.6% | (many diffs) |
| 5 | mouse | 97165 | Hmgb2 | high mobility group box 2 | XM_006509524.2 | 89.8% | 96.6% | (many diffs) |
| 6 | mouse | 100041490 | Gm20875 | predicted gene, 20875 | XR_140686.3 | 62.2% | (many diffs) | |
| 7 | mouse | 666390 | Gm13237 | predicted gene 13237 | XR_140685.5 | 53.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 693
- ORF length:
- 627
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg taaaggagac cccaacaagc cgcggggcaa aatgtcctcg tacgccttct 121 tcgtgcagac ctgccgggaa gagcacaaga agaaacaccc ggactcttcc gtcaatttcg 181 cggaattctc caagaagtgt tcggagagat ggaagaccat gtctgcaaag gagaagtcga 241 agtttgaaga tatggcaaaa agtgacaaag ctcgctatga cagggagatg aaaaattacg 301 ttcctcccaa aggtgataag aaggggaaga aaaaggacCC CAATGCTCCT AAAAGGCCAC 361 CATCTGCCTT CTTCCTGTTT TGCTCTGAAC ATCGCCCAAA GATCAAAAGT GAACACCCTG 421 GCCTATCCAT TGGGGATACT GCAAAGAAAT TGGGTGAAAT GTGGTCTGAG CAGTCAGCCA 481 AAGATAAACA ACCATATGAA CAGAAAGCAG CTAAGCTAAA GGAGAAATAT GAAAAGGATA 541 TTGCTGCATA TCGTGCCAAG GGCAAAAGTG AAGCAGGAAA GAAGGGCCCT GGCAGGCCAA 601 CAGGCTCAAA GAAGAAGAAC GAACCAGAAG ATGAGGAGGA GGAGGAGGAA GAAGAAGATG 661 AAGATGAGGA GGAAGAGGAT GAAGATGAAG AATACCCAAC TTTCTTGTAC AAAGTGGTTG 721 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 781 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 841 GTGCCCACAA AACATGTAAA GTACGCGTTA AGTCgacaat caacctctgg attacaaaat 901 ttgtgaaaga tt