Construct: ORF TRCN0000472080
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000666.1_s317c1
- Derived from:
- ccsbBroadEn_00505
- DNA Barcode:
- CGCAGGTTGCACAGAATGCTCGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPB41L1 (2036)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472080
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | NM_001258331.2 | 100% | 100% | |
| 2 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | NM_177996.2 | 100% | 100% | |
| 3 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027714.2 | 96.1% | 96.1% | 1_93del |
| 4 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027713.2 | 92.9% | 92.9% | 1_93del;2055_2138del |
| 5 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027712.2 | 91.6% | 91.6% | 1_93del;1355_1390del;2091_2174del |
| 6 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_011528683.1 | 91.3% | 91.3% | 1_186del;1448_1483del |
| 7 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_011528681.1 | 89.7% | 89.7% | 1_186del;2148_2228del |
| 8 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_011528682.1 | 89.6% | 89.6% | 1_186del;1298_1299insAGC;2145_2225del |
| 9 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027715.1 | 89.5% | 89.5% | 1_186del;1298_1299insAGC;2145_2228del |
| 10 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | NM_001258329.1 | 88.5% | 88.5% | 1_186del;1448_1483del;2184_2264del |
| 11 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027716.1 | 88.5% | 88.5% | 1_186del;1448_1483del;2184_2264del |
| 12 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027717.2 | 88.5% | 88.5% | 1_186del;1448_1483del;2184_2264del |
| 13 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | NM_012156.2 | 88.4% | 88.4% | 1_186del;1448_1483del;2184_2267del |
| 14 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_024451854.1 | 81.1% | 81.1% | 1262_1297del;1478_1479ins411 |
| 15 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | NM_001258330.1 | 76.6% | 76.6% | 1_93del;1535_1536ins411;1644_1727del |
| 16 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027719.2 | 76.6% | 76.6% | 1_93del;1535_1536ins411;1644_1727del |
| 17 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027720.1 | 76.3% | 76.3% | 1_186del;1628_1629ins411 |
| 18 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027718.2 | 75.5% | 75.5% | (many diffs) |
| 19 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_011528685.1 | 72.8% | 72.8% | (many diffs) |
| 20 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_011528677.1 | 52% | 52% | 1262_1297del;1482_3518del;4035_4118del |
| 21 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027711.1 | 52% | 52% | 1262_1297del;1482_3518del;4035_4118del |
| 22 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_024451852.1 | 52% | 52% | 1262_1297del;1482_3518del;4035_4118del |
| 23 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_024451853.1 | 52% | 52% | 1262_1297del;1482_3518del;4035_4118del |
| 24 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_024451851.1 | 50.3% | 50.3% | (many diffs) |
| 25 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_011528671.1 | 49.9% | 49.9% | (many diffs) |
| 26 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_011528673.1 | 49.9% | 49.9% | (many diffs) |
| 27 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_011528667.1 | 49.1% | 49.1% | (many diffs) |
| 28 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | NM_001291121.1 | 82% | 86.6% | (many diffs) |
| 29 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | NM_001006664.3 | 80.9% | 85.4% | (many diffs) |
| 30 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | NM_001291120.1 | 80.9% | 85.4% | (many diffs) |
| 31 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | NM_013510.4 | 80.9% | 85.4% | (many diffs) |
| 32 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_017315393.1 | 80.8% | 85.3% | (many diffs) |
| 33 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_017315394.1 | 80% | 84.5% | (many diffs) |
| 34 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | NM_001291122.1 | 71.7% | 72.1% | (many diffs) |
| 35 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | NM_001291123.1 | 71.6% | 72% | (many diffs) |
| 36 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_011239281.1 | 70.7% | 71.1% | (many diffs) |
| 37 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_011239277.1 | 45.1% | 47.6% | (many diffs) |
| 38 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_006498711.3 | 44.7% | 47.2% | (many diffs) |
| 39 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_006498713.3 | 44.7% | 47.2% | (many diffs) |
| 40 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_006498714.3 | 44.7% | 47.2% | (many diffs) |
| 41 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_011239280.2 | 44.7% | 47.2% | (many diffs) |
| 42 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_017315383.1 | 44.7% | 47.2% | (many diffs) |
| 43 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_011239275.2 | 44.7% | 47.2% | (many diffs) |
| 44 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_011239278.1 | 44.6% | 47.1% | (many diffs) |
| 45 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_006498708.3 | 44.4% | 46.9% | (many diffs) |
| 46 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_011239276.1 | 44.3% | 46.7% | (many diffs) |
| 47 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_011239279.1 | 43.2% | 45.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2403
- ORF length:
- 2337
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggagaaggac tacagtgagg ccgatggcct ttcggagagg accacgccca 121 gcaaggccca gaaatcgccc cagaagattg ccaagaaata caagagtgcc atctgccggg 181 tcactctgct tgatgcctcg gagtatgagt gtgaggtgga gaaacatggc cggggccagg 241 tgctgtttga cctggtctgt gaacacctca acctcctaga gaaggactac ttcggcctga 301 ccttctgtga tgctgacagc cagaagaact ggctggaccc ctccaaggag atcaagaagc 361 agatccggag tagcccctgg aattttgcct tcacagtcaa gttctacccg cctgatcctg 421 cccagctgac agaagacatc acaagatact acctgtgcct gcagctgcgg gcagacatca 481 tcacgggccg gctgccatgc tcctttgtca cgcatgccct actgggctcc tacgctgtgc 541 aggctgagct gggtgactat gatgctgagg agcatgtggg caactatgtc agcgagctcc 601 gcttcgcccc taaccagacc cgggagctgg aggagaggat catggagctg cataagacat 661 atagggggat gaccccggga gaagcagaaa tccacttctt agagaatgcc aagaagcttt 721 ccatgtacgg agtagacctg caccatgcca aggactctga gggcatcgac atcatgttag 781 gcgtttgtgc caatggcctg ctcatctacc gggaccggct gagaatcaac cgctttgcct 841 ggcccaagat cctcaagatc tcctacaaga ggagtaactt ctatatcaag atccggcctg 901 gggagtatga gcaatttgag agcacaattg gctttaagct cccaaaccac cggtcagcca 961 agagactgtg gaaggtctgc atcgagcatc atacattctt ccggctggtg tcccctgagc 1021 ccccacccaa gggcttcctg gtgatgggct ccaagttccg gtacagtggg aggacccagg 1081 cacagactcg ccaggccagc gccctcattg accggcctgc acccttcttt gagcgttctt 1141 ccagcaaacg gtacaccatg tcccgcagcc ttgatggagc agagttctcc cgcccagcct 1201 cggtcagcga gaaccatgat gcagggcctg acggtgacaa gcgggatgag gatggcgagt 1261 ctggggggca acggtcagag gctgaggagg gagaggtcag gactccaacc aagatcaagg 1321 agctaaagtt cttagacaag ccagaagatg tcttgctgaa gcaccaggcc agcatcaatg 1381 agctcaaaag gaccctgaag gagcccaaca gcaaactcat ccaccgggat cgagactggg 1441 aacgggagcg caggctgccc tcctcccccg cctccccctc ccccaagggc acccctgaga 1501 aagccaatga gagagcaggg ctgagggagg gctccgagga gaaagtcaaa ccaccacgtc 1561 cccgggcccc agagagtgac acaggcgatg aggaccagga ccaggagagg gacacggtgt 1621 tcctgaagga caaccacctg gccattgagc gcaagtgctc cagcatcacg gtcagctcta 1681 cgtctagcct ggaggctgag gtggacttca cggtcattgg tgactaccat ggcagcgcct 1741 tcgaagactt ctcccgcagc ctgcctgagc tcgaccggga caaaagcgac tcggacactg 1801 agggcctgct gttctcccgg gatctcaaca agggggcccc cagccaggat gatgagtctg 1861 ggggcattga ggacagcccg gatcgagggg cctgctccac cccggatatg ccccagtttg 1921 agcccgtgaa aacagaaacc atgactgtca gcagtctggc cattagaaag aagattgagc 1981 cggaggccgt actgcagacc agagtctccg ctatggataa cacccaggag aacagtctca 2041 agtccgggaa gggggcagct gccatgatcc caggcccaca gacggtggcc acggaaatcc 2101 gttctctttc tccgatcatc gggaaagatg tcctcaccag cacctacggc gccacTGCGG 2161 AAACCCTCTC AACCTCCACC ACCACCCATG TCACCAAAAC TGTGAAAGGA GGGTTTTCTG 2221 AGACAAGGAT CGAGAAGCGA ATCATCATTA CTGGGGATGA AGATGTCGAT CAAGACCAGG 2281 CCCTGGCTTT GGCCATCAAG GAGGCCAAAC TGCAGCATCC TGATATGCTG GTAACCAAAG 2341 CTGTCGTATA CAGAGAAACA GACCCATCCC CAGAGGAGAG GGACAAGAAG CCACAGGAAT 2401 CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2461 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2521 GGCTTTATAT ATCTTGTGGA AAGGACGACG CAGGTTGCAC AGAATGCTCG GTACGCGTTA 2581 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt