Construct: ORF TRCN0000472188
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015257.1_s317c1
- Derived from:
- ccsbBroadEn_04236
- DNA Barcode:
- TTGCTGTTCCCCCGCACCTGTGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STMN4 (81551)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472188
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81551 | STMN4 | stathmin 4 | NM_030795.4 | 100% | 100% | |
| 2 | human | 81551 | STMN4 | stathmin 4 | NM_001283053.2 | 93% | 91.6% | (many diffs) |
| 3 | human | 81551 | STMN4 | stathmin 4 | NM_001283054.2 | 87.5% | 87.5% | 108_109ins81 |
| 4 | human | 81551 | STMN4 | stathmin 4 | XM_005273652.4 | 81.9% | 79.4% | (many diffs) |
| 5 | human | 81551 | STMN4 | stathmin 4 | XM_005273655.5 | 80.5% | 79.1% | (many diffs) |
| 6 | human | 81551 | STMN4 | stathmin 4 | NM_001283055.2 | 76.4% | 75% | (many diffs) |
| 7 | human | 81551 | STMN4 | stathmin 4 | XR_002956647.1 | 36.8% | (many diffs) | |
| 8 | mouse | 56471 | Stmn4 | stathmin-like 4 | NM_001310522.1 | 88.8% | 97.6% | (many diffs) |
| 9 | mouse | 56471 | Stmn4 | stathmin-like 4 | XM_006519316.2 | 82.4% | 89.3% | (many diffs) |
| 10 | mouse | 56471 | Stmn4 | stathmin-like 4 | XM_006519317.2 | 82.4% | 89.3% | (many diffs) |
| 11 | mouse | 56471 | Stmn4 | stathmin-like 4 | NM_019675.3 | 77.3% | 87% | (many diffs) |
| 12 | mouse | 56471 | Stmn4 | stathmin-like 4 | NM_001310523.1 | 70.8% | 78.7% | (many diffs) |
| 13 | mouse | 56471 | Stmn4 | stathmin-like 4 | XM_017316097.1 | 70.8% | 78.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 714
- ORF length:
- 648
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ccttgctgcc tacaaagaga agatgaagga gctcccgctg gtgtccttgt 121 tctgctcctg cttcctggcc gatcccctga ataagtcgtc ctacaaatat gaaggctggt 181 gtgggagaca gtgtaggagg aaggatgaaa gccagcggaa agacagtgct gactggagag 241 aaagaagagc tcaggcagac acggtggacc tgaattggtg cgtcatttcc gacatggaag 301 tcatcgagct gaacaaatgc acctcgggcc aaTCCTTTGA AGTCATCCTG AAGCCACCCT 361 CCTTTGATGG GGTTCCCGAG TTCAACGCCT CCCTGCCAAG GCGGCGAGAC CCATCCCTGG 421 AAGAGATCCA GAAGAAACTA GAAGCGGCTG AGGAGCGAAG GAAGTACCAG GAAGCGGAGC 481 TCCTGAAACA CCTAGCAGAG AAACGGGAAC ATGAGAGAGA GGTGATCCAA AAGGCCATTG 541 AGGAAAACAA CAACTTCATC AAGATGGCTA AGGAAAAACT GGCCCAGAAG ATGGAATCCA 601 ACAAGGAGAA CAGGGAGGCC CACCTCGCCG CCATGTTGGA ACGGCTGCAA GAGAAGGACA 661 AGCACGCCGA GGAGGTGCGG AAAAACAAGG AGCTGAAGGA AGAGGCCTCC AGGTACCCAA 721 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 781 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 841 TATCTTGTGG AAAGGACGAT TGCTGTTCCC CCGCACCTGT GTGACGCGTT AAGTCgacaa 901 tcaacctctg gattacaaaa tttgtgaaag att