Construct: ORF TRCN0000472210
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008407.1_s317c1
- Derived from:
- ccsbBroadEn_00587
- DNA Barcode:
- TTCATCCTCTCAACCTCCAGTACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FOSL2 (2355)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472210
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 2355 | FOSL2 | FOS like 2, AP-1 transcript... | NM_005253.4 | 100% | 100% | |
2 | human | 2355 | FOSL2 | FOS like 2, AP-1 transcript... | XM_006711976.3 | 95% | 95% | 462_512del |
3 | human | 2355 | FOSL2 | FOS like 2, AP-1 transcript... | XM_006711977.3 | 83.6% | 83.6% | 0_1ins117;345_395del |
4 | human | 2355 | FOSL2 | FOS like 2, AP-1 transcript... | XM_005264231.4 | 47.3% | 51% | 463_565del;615_616ins466 |
5 | mouse | 14284 | Fosl2 | fos-like antigen 2 | NM_008037.4 | 89.3% | 94.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1044
- ORF length:
- 978
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta ccaggattat cccgggaact ttgacacctc gtcccggggc agcagcggct 121 ctcctgcgca cgccgagtcc tactccagcg gcggcggcgg ccagcagaaa ttccgggtag 181 atatgcctgg ctcaggcagt gcattcatcc ccaccatcaa cgccatcacg accagccagg 241 acctgcagtg gatggtgcag cccacagtga tcacctccat gtccaaccca taccctcgct 301 cgcaccccta cagccccctg ccgggcctgg cctctgtccc tggacacatg gccctcccaa 361 gacctggcgt gatcaagacc attggcacca ccgtgggccg caggaggaga gatgagcagc 421 tgtctcctga agaggaggag aagcgtcgca tccggcggga gaggaacaag ctggctgcag 481 ccaagtgccg gaaccgacgc cgggagctga cagagaagct gcaggcggag acagaggagc 541 tggaggagga gaagtcaggc ctgcagaagg agattgcTGA GCTGCAGAAG GAGAAGGAGA 601 AGCTGGAGTT CATGTTGGTG GCTCACGGCC CAGTGTGCAA GATTAGCCCC GAGGAGCGCC 661 GATCGCCCCC AGCCCCTGGG CTGCAGCCCA TGCGCAGTGG GGGTGGCTCG GTGGGCGCTG 721 TAGTGGTGAA ACAGGAGCCC CTGGAAGAGG ACAGCCCCTC GTCCTCGTCG GCGGGGCTGG 781 ACAAGGCCCA GCGCTCTGTC ATCAAGCCCA TCAGCATTGC TGGGGGCTTC TACGGTGAGG 841 AGCCCCTGCA CACCCCCATC GTGGTGACCT CCACACCTGC TGTCACTCCG GGCACCTCGA 901 ACCTCGTCTT CACCTATCCT AGCGTCCTGG AGCAGGAGTC ACCCGCATCT CCCTCCGAAT 961 CCTGCTCCAA GGCTCACCGC AGAAGCAGTA GCAGCGGGGA CCAATCATCA GACTCCTTGA 1021 ACTCCCCCAC TCTGCTGGCT CTGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1081 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1141 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TCATCCTCTC 1201 AACCTCCAGT ACCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1261 att