Construct: ORF TRCN0000472237
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009579.1_s317c1
- Derived from:
- ccsbBroadEn_15311
- DNA Barcode:
- AATCACACCAAATATCAACTATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LCE2A (353139)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472237
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 353139 | LCE2A | late cornified envelope 2A | NM_178428.4 | 48.7% | 48.1% | 149G>A;157_318del |
| 2 | human | 353140 | LCE2C | late cornified envelope 2C | NM_178429.3 | 42.4% | 43.6% | (many diffs) |
| 3 | human | 26239 | LCE2B | late cornified envelope 2B | NM_014357.5 | 42.4% | 42.7% | (many diffs) |
| 4 | human | 353141 | LCE2D | late cornified envelope 2D | NM_178430.4 | 41.8% | 43.6% | (many diffs) |
| 5 | human | 254910 | LCE5A | late cornified envelope 5A | NM_178438.5 | 39.2% | 38.9% | (many diffs) |
| 6 | human | 353135 | LCE1E | late cornified envelope 1E | NM_178353.2 | 34.4% | 35.2% | (many diffs) |
| 7 | human | 353132 | LCE1B | late cornified envelope 1B | NM_178349.2 | 33.7% | 34.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 317
- ORF end:
- 473
- ORF length:
- 156
- Sequence:
-
1 GTCGCTTCAT GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG 61 AGCTTTTGGA GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC 121 CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG 181 GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA 241 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC GATTGATCAA CAAGTTTGTA 301 CAAAAAAGTT GGCACCATGT CCTGCCAGCA AAACCAGCAG CAGTGCCAGC CCCCTCCCAA 361 GTGCCCCCCA AAATGCCCAC CCAAGTGTCC TCCAAAGTGC CGACCTCAGT GCCCAGCCCC 421 ATGCCCACCT CCAGTCTCTT CCTGCTGTGG TCCCAGCTCT GGGGACTGCT GCTTGCCAAC 481 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 541 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 601 ATCTTGTGGA AAGGACGAAA TCACACCAAA TATCAACTAT ATACGCGTTA AGTCgacaat 661 caacctctgg attacaaaat ttgtgaaaga tt