Construct: ORF TRCN0000472254
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001479.1_s317c1
- Derived from:
- ccsbBroadEn_14348
- DNA Barcode:
- GTTTATTACAACGAGACATCGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EGLN2 (112398)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472254
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112398 | EGLN2 | egl-9 family hypoxia induci... | NM_053046.4 | 30.6% | 30.4% | 1_846del;856T>G |
| 2 | human | 112398 | EGLN2 | egl-9 family hypoxia induci... | NM_080732.4 | 30.6% | 30.4% | 1_846del;856T>G |
| 3 | human | 100529264 | RAB4B-EGLN2 | RAB4B-EGLN2 readthrough (NM... | NR_037791.1 | 13.1% | 1_1894del;1904T>G;2270_2849del | |
| 4 | mouse | 112406 | Egln2 | egl-9 family hypoxia-induci... | XM_017321929.1 | 39.5% | 40.9% | (many diffs) |
| 5 | mouse | 112406 | Egln2 | egl-9 family hypoxia-induci... | NM_053208.4 | 28.1% | 29.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 441
- ORF length:
- 375
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggcgggttac ccaggcaacg ggcTCGGGTA CGTAAGGCAC GTTGACAATC 121 CCCACGGCGA TGGGCGCTGC ATCACCTGTA TCTATTACCT GAATCAGAAC TGGGACGTTA 181 AGGTGCATGG CGGCCTGCTG CAGATCTTCC CTGAGGGCCG GCCCGTGGTA GCCAACATCG 241 AGCCACTCTT TGACCGGTTG CTCATTTTCT GGTCTGACCG GCGGAACCCC CACGAGGTGA 301 AGCCAGCCTA TGCCACCAGG TACGCCATCA CTGTCTGGTA TTTTGATGCC AAGGAGCGGG 361 CAGCAGCCAA AGACAAGTAT CAGCTAGCAT CAGGACAGAA AGGTGTCCAA GTACCTGTAT 421 CACAGCCGCC TACGCCCACC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 481 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTTAACTT 541 GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTT TATTACAACG 601 AGACATCGAT CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat 661 t