Construct: ORF TRCN0000472306
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010253.1_s317c1
- Derived from:
- ccsbBroadEn_06494
- DNA Barcode:
- ACCGACGGTGTTGTACGCAGAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLRC1 (3821)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472306
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3821 | KLRC1 | killer cell lectin like rec... | NM_213657.2 | 99.8% | 99.5% | 12A>T |
| 2 | human | 3821 | KLRC1 | killer cell lectin like rec... | NM_007328.4 | 99.6% | 99% | 12A>T;86A>G |
| 3 | human | 3821 | KLRC1 | killer cell lectin like rec... | NM_213658.2 | 92.1% | 91.8% | 12A>T;284_337del |
| 4 | human | 3821 | KLRC1 | killer cell lectin like rec... | NM_002259.5 | 91.9% | 91.4% | 12A>T;86A>G;284_337del |
| 5 | human | 3821 | KLRC1 | killer cell lectin like rec... | NM_001304448.1 | 89.9% | 89.6% | 12A>T;284_337del;684_685insTGTAAGCATAAGCTT |
| 6 | human | 3821 | KLRC1 | killer cell lectin like rec... | XM_024448973.1 | 89.8% | 89.2% | (many diffs) |
| 7 | human | 3822 | KLRC2 | killer cell lectin like rec... | NM_002260.3 | 82.8% | 72.7% | (many diffs) |
| 8 | human | 3823 | KLRC3 | killer cell lectin like rec... | NM_002261.2 | 76.2% | 62.9% | (many diffs) |
| 9 | human | 3823 | KLRC3 | killer cell lectin like rec... | NM_007333.2 | 71.8% | 59.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 711
- ORF length:
- 645
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga taaccatgga gtaatctact cagacctgaa tctgccccca aacccaaaga 121 ggcagcaacg aaaacctaaa ggcaataaaa gctccatttt agcaactgaa caggaaataa 181 cctatgcgga attaaaccTT CAAAAAGCTT CTCAGGATTT TCAAGGGAAT GACAAAACCT 241 ATCACTGCAA AGATTTACCA TCAGCTCCAG AGAAGCTCAT TGTTGGGATC CTGGGAATTA 301 TCTGTCTTAT CTTAATGGCC TCTGTGGTAA CGATAGTTGT TATTCCCTCA CGTCATTGTG 361 GCCATTGTCC TGAGGAGTGG ATTACATATT CCAACAGTTG TTACTACATT GGTAAGGAAA 421 GAAGAACTTG GGAAGAGAGT TTGCTGGCCT GTACTTCGAA GAACTCCAGT CTGCTTTCTA 481 TAGATAATGA AGAAGAAATG AAATTTCTGT CCATCATTTC ACCATCCTCA TGGATTGGTG 541 TGTTTCGTAA CAGCAGTCAT CATCCATGGG TGACAATGAA TGGTTTGGCT TTCAAACATG 601 AGATAAAAGA CTCAGATAAT GCTGAACTTA ACTGTGCAGT GCTACAAGTA AATCGACTTA 661 AATCAGCCCA GTGTGGATCT TCAATAATAT ATCATTGTAA GCATAAGCTT TACCCAACTT 721 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 781 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 841 CTTGTGGAAA GGACGAACCG ACGGTGTTGT ACGCAGAAAC ACGCGTTAAG TCgacaatca 901 acctctggat tacaaaattt gtgaaagatt