Construct: ORF TRCN0000472377
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016444.1_s317c1
- Derived from:
- ccsbBroadEn_06892
- DNA Barcode:
- TCTGGATAGTTTGTGCCCCTCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS4X (6191)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472377
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6191 | RPS4X | ribosomal protein S4 X-linked | NM_001007.5 | 99.2% | 100% | (many diffs) |
| 2 | human | 6192 | RPS4Y1 | ribosomal protein S4 Y-link... | NM_001008.4 | 82.7% | 92.7% | (many diffs) |
| 3 | human | 140032 | RPS4Y2 | ribosomal protein S4 Y-link... | NM_001039567.3 | 82.6% | 91.6% | (many diffs) |
| 4 | mouse | 20102 | Rps4x | ribosomal protein S4, X-linked | NM_009094.2 | 90.2% | 100% | (many diffs) |
| 5 | mouse | 20102 | Rps4x | ribosomal protein S4, X-linked | XM_011247553.2 | 84.5% | 93.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 858
- ORF length:
- 789
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcccggggc cccaagaagc atctgaagcg ggtggcagct ccaaagcatt 121 ggatgctgga taaattgacc ggtgtgtttg ctcctcgtcc atccaccggt ccccacaagt 181 tgagagagtg tctccccctc atcattttcc tgaggaacag acttaagtat gccctgacag 241 gagatgaagt aaagaagatt tgcatgcagc ggttcattaa aatcgatggc aaggtccgaa 301 ctgatataac ctaccctgct ggattcatgg atgtcatcag cattgacaag acgggagaga 361 atttccgtcT GATCTATGAC ACCAAGGGTC GCTTTGCTGT ACATCGTATT ACACCTGAGG 421 AGGCCAAGTA CAAGTTGTGC AAAGTGAGAA AGATCTTTGT GGGCACAAAA GGAATCCCTC 481 ATCTGGTGAC TCATGATGCC CGCACCATCC GCTACCCCGA TCCCCTCATC AAGGTGAATG 541 ATACCATTCA GATTGATTTA GAGACTGGCA AGATTACTGA TTTCATCAAG TTCGACACTG 601 GTAACCTGTG TATGGTGACT GGAGGTGCTA ACCTAGGAAG AATTGGTGTG ATCACCAACA 661 GAGAGAGGCA CCCTGGATCT TTTGACGTGG TTCACGTGAA AGATGCCAAT GGCAACAGCT 721 TTGCCACTCG ACTTTCCAAC ATTTTTGTTA TTGGCAAGGG CAACAAACCA TGGATTTCTC 781 TTCCCCGAGG AAAGGGTATC CGCCTCACCA TTGCTGAAGA GAGAGACAAA AGACTGGCTG 841 CCAAACAGAG CAGTGGCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 961 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCTGGAT AGTTTGTGCC 1021 CCTCAACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt