Construct: ORF TRCN0000472421
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010104.1_s317c1
- Derived from:
- ccsbBroadEn_09752
- DNA Barcode:
- TCCAGGGGAAATTTCGAATCAGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DENND1B (163486)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472421
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 163486 | DENND1B | DENN domain containing 1B | NM_144977.5 | 99.9% | 99.7% | 4G>A |
| 2 | human | 163486 | DENND1B | DENN domain containing 1B | NM_001300858.2 | 91% | 87.1% | (many diffs) |
| 3 | human | 163486 | DENND1B | DENN domain containing 1B | XM_011509251.3 | 82.4% | 79.3% | (many diffs) |
| 4 | human | 163486 | DENND1B | DENN domain containing 1B | NM_001195215.2 | 54.4% | 53% | (many diffs) |
| 5 | human | 163486 | DENND1B | DENN domain containing 1B | XM_011509248.2 | 53.2% | 51.5% | (many diffs) |
| 6 | human | 163486 | DENND1B | DENN domain containing 1B | XM_006711192.2 | 52.2% | 49.2% | (many diffs) |
| 7 | human | 163486 | DENND1B | DENN domain containing 1B | XM_006711193.3 | 52.2% | 49.2% | (many diffs) |
| 8 | human | 163486 | DENND1B | DENN domain containing 1B | XM_011509249.2 | 52.2% | 49.2% | (many diffs) |
| 9 | human | 163486 | DENND1B | DENN domain containing 1B | XM_017000470.1 | 52.2% | 49.2% | (many diffs) |
| 10 | human | 163486 | DENND1B | DENN domain containing 1B | XM_005244931.2 | 51.8% | 50.5% | (many diffs) |
| 11 | human | 163486 | DENND1B | DENN domain containing 1B | XM_011509246.2 | 51.1% | 49.3% | (many diffs) |
| 12 | human | 163486 | DENND1B | DENN domain containing 1B | XM_017000471.1 | 49.7% | 46.7% | (many diffs) |
| 13 | human | 163486 | DENND1B | DENN domain containing 1B | XM_011509247.1 | 48.7% | 46.9% | (many diffs) |
| 14 | human | 163486 | DENND1B | DENN domain containing 1B | XM_024453626.1 | 28.6% | 27.4% | (many diffs) |
| 15 | human | 163486 | DENND1B | DENN domain containing 1B | XM_024453627.1 | 17.9% | 16.7% | (many diffs) |
| 16 | human | 163486 | DENND1B | DENN domain containing 1B | XM_006711194.3 | 15% | 13.8% | (many diffs) |
| 17 | human | 163486 | DENND1B | DENN domain containing 1B | NR_125340.2 | 14% | (many diffs) | |
| 18 | mouse | 329260 | Dennd1b | DENN/MADD domain containing 1B | XM_006529716.3 | 48.1% | 48.9% | (many diffs) |
| 19 | mouse | 329260 | Dennd1b | DENN/MADD domain containing 1B | NM_001166501.1 | 46% | 47% | (many diffs) |
| 20 | mouse | 329260 | Dennd1b | DENN/MADD domain containing 1B | XM_006529717.2 | 44% | 44.7% | (many diffs) |
| 21 | mouse | 329260 | Dennd1b | DENN/MADD domain containing 1B | XM_006529718.2 | 39.5% | 37.9% | (many diffs) |
| 22 | mouse | 329260 | Dennd1b | DENN/MADD domain containing 1B | XM_006529719.2 | 37.3% | 36% | (many diffs) |
| 23 | mouse | 329260 | Dennd1b | DENN/MADD domain containing 1B | NM_181347.3 | 37.3% | 38.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1344
- ORF length:
- 1278
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ctgcaggacc aaggcaaatc cagacagaac ctttgacttg gtgttgaaag 121 tgaaatgtca tgcctctgaa aatgaagatc ctgtggtatt gtggaaattc ccagaggact 181 ttggagacca ggaaatacta cagagtgtgc caaagttctg ttttcccttt gacgttgaaa 241 gggtgtctca gaatcaagtt ggacagcact ttacctttgt actgacagac attgaaagta 301 aacagagatt tggattctgc agactgacgt caggaggcac aatttgttta tgcatcctta 361 gttaccttcc ctggtttgaa gtgtattaca agcttctaaa tactcttgca gattacttgg 421 ctaaggaact ggaaaatgat ttgaatgaaa ctctcagatc actgtataac cacccagtac 481 caaaggcaaa tactcctgta aatttgagtg tgaaccaaga gatatttatt gcctgtgagc 541 aagttctgaa agatcagcct gctctagtac cgcattccta cttcattgcc cctgatgtaa 601 ctggactccc aacaataccc gagagtagaa atcttacaga atattttgtt gccgtggatg 661 tgaacaacat gctgcagctg tatgccagta tgctgcatga aaggcgcatc gtgattatct 721 cgagcaaatt aagcacttta actgcctgta tccatggatc agctgctctt ctatacccaa 781 tgtattggca acacatatac atcccagtgc ttcctccaca cctgctggac tactgctgtg 841 ccccaatgcc atacctgatt ggaatacact ccagcctcat agagagagtg aaaaacaaaT 901 CATTGGAAGA TGTTGTTATG TTAAATGTTG ATACAAACAC ATTAGAATCA CCATTTAGTG 961 ACTTGAACAA CCTACCAAGT GATGTGGTCT CGGCCTTGAA AAATAAACTG AAGAAGCAGT 1021 CTACAGCTAC GGGTGATGGA GTAGCTAGGG CCTTTCTTAG AGCACAGGCT GCTTTGTTTG 1081 GATCCTACAG AGATGCACTG AGATACAAAC CTGGTGAGCC CATCACTTTC TGTGAGGAGA 1141 GTTTTGTAAA GCACCGCTCA AGCGTGATGA AACAGTTCCT GGAAACTGCC ATTAACCTCC 1201 AGCTTTTTAA GCAGTTTATC GATGGTCGAC TGGCAAAACT AAATGCAGGA AGGGGTTTCT 1261 CTGATGTATT TGAAGAAGAG ATCACTTCAG GTGGCTTTTG TGGAGGTAAA GACAAGTTAC 1321 AATATGATTA TCCATTTTCT CAATACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1381 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1441 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CCAGGGGAAA 1501 TTTCGAATCA GTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1561 att