Construct: ORF TRCN0000472437
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011044.1_s317c1
- Derived from:
- ccsbBroadEn_01912
- DNA Barcode:
- ACTCCACAACGATCTTTCCTTCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H4C11 (8363)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472437
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8362 | H4C12 | H4 clustered histone 12 | NM_003541.2 | 100% | 100% | |
| 2 | human | 8363 | H4C11 | H4 clustered histone 11 | NM_021968.3 | 100% | 100% | |
| 3 | human | 8294 | H4C9 | H4 clustered histone 9 | NM_003495.2 | 90.2% | 100% | (many diffs) |
| 4 | human | 554313 | H4C15 | H4 clustered histone 15 | NM_001034077.4 | 85.8% | 100% | (many diffs) |
| 5 | human | 8370 | H4C14 | H4 clustered histone 14 | NM_003548.2 | 85.8% | 100% | (many diffs) |
| 6 | human | 8359 | H4C1 | H4 clustered histone 1 | NM_003538.3 | 85.7% | 100% | (many diffs) |
| 7 | human | 8368 | H4C13 | H4 clustered histone 13 | NM_003546.2 | 85.7% | 100% | (many diffs) |
| 8 | human | 8367 | H4C5 | H4 clustered histone 5 | NM_003545.3 | 85.4% | 100% | (many diffs) |
| 9 | human | 8366 | H4C2 | H4 clustered histone 2 | NM_003544.2 | 84.1% | 100% | (many diffs) |
| 10 | human | 8360 | H4C4 | H4 clustered histone 4 | NM_003539.3 | 83.8% | 100% | (many diffs) |
| 11 | human | 8361 | H4C6 | H4 clustered histone 6 | NM_003540.3 | 83.8% | 100% | (many diffs) |
| 12 | human | 8365 | H4C8 | H4 clustered histone 8 | NM_003543.3 | 83.4% | 100% | (many diffs) |
| 13 | human | 121504 | H4-16 | H4 histone 16 | NM_175054.2 | 83.1% | 100% | (many diffs) |
| 14 | human | 8364 | H4C3 | H4 clustered histone 3 | NM_003542.3 | 80.6% | 100% | (many diffs) |
| 15 | mouse | 69386 | Hist1h4h | histone cluster 1, H4h | NM_153173.4 | 90.9% | 100% | (many diffs) |
| 16 | mouse | 326620 | Hist1h4b | histone cluster 1, H4b | NM_178193.2 | 90.6% | 100% | (many diffs) |
| 17 | mouse | 100041230 | Hist1h4m | histone cluster 1, H4m | NM_001195421.1 | 89.9% | 100% | (many diffs) |
| 18 | mouse | 319156 | Hist1h4d | histone cluster 1, H4d | NM_175654.2 | 89.9% | 100% | (many diffs) |
| 19 | mouse | 319161 | Hist1h4n | histone cluster 1, H4n | NM_175657.2 | 89.9% | 100% | (many diffs) |
| 20 | mouse | 326619 | Hist1h4a | histone cluster 1, H4a | NM_178192.2 | 89.9% | 100% | (many diffs) |
| 21 | mouse | 319159 | Hist1h4j | histone cluster 1, H4j | NM_178210.2 | 89.9% | 100% | (many diffs) |
| 22 | mouse | 319160 | Hist1h4k | histone cluster 1, H4k | NM_178211.2 | 89.6% | 100% | (many diffs) |
| 23 | mouse | 319157 | Hist1h4f | histone cluster 1, H4f | NM_175655.2 | 89.3% | 100% | (many diffs) |
| 24 | mouse | 319158 | Hist1h4i | histone cluster 1, H4i | NM_175656.3 | 89.3% | 100% | (many diffs) |
| 25 | mouse | 319155 | Hist1h4c | histone cluster 1, H4c | NM_178208.2 | 88.9% | 100% | (many diffs) |
| 26 | mouse | 97122 | Hist2h4 | histone cluster 2, H4 | NM_033596.3 | 87% | 100% | (many diffs) |
| 27 | mouse | 320332 | Hist4h4 | histone cluster 4, H4 | NM_175652.3 | 85.4% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 111
- ORF end:
- 420
- ORF length:
- 309
- Sequence:
-
1 ATCTTGGTTC ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGGT 61 GTCGTGAGGC TAGCATCGAT TGATCAACAA GTTTGTACAA AAAAGTTGGC ATGTCTGGCC 121 GCGGCAAAGG CGGGAAGGGT CTTGGCAAAG GCGGCGCTAA GCGCCACCGT AAAGTACTGC 181 GCGACAATAT CCAGGGCATC ACCAAGCCGG CCATCCGGCG CCTTGCTCGC CGCGGCGGCG 241 TGAAGCGCAT CTCCGGCCTC ATCTACGAGG AGACTCGCGG GGTGCTGAAG GTGTTCCTGG 301 AGAACGTGAT CCGGGACGCC GTGACCTATA CAGAGCACGC CAAGCGCAAG ACGGTCACCG 361 CCATGGATGT GGTCTACGCG CTCAAGCGCC AGGGCCGCAC CCTCTACGGT TTCGGTGGTT 421 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 481 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 541 TTTATATATC TTGTGGAAAG GACGAACTCC ACAACGATCT TTCCTTCATA CGCGTTAAGT 601 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt