Construct: ORF TRCN0000472494

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017817.1_s317c1
Derived from:
ccsbBroadEn_01262
DNA Barcode:
TCCGTGTTGCGGATAGAGAATCTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PPP2CB (5516)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472494
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5516 PPP2CB protein phosphatase 2 catal... NM_001009552.2 100% 100%
2 human 5515 PPP2CA protein phosphatase 2 catal... NM_002715.4 83.1% 97.4% (many diffs)
3 human 5515 PPP2CA protein phosphatase 2 catal... NM_001355019.1 65% 78.6% (many diffs)
4 mouse 19053 Ppp2cb protein phosphatase 2 (form... NM_017374.3 92.1% 100% (many diffs)
5 mouse 19052 Ppp2ca protein phosphatase 2 (form... NM_019411.4 80.9% 97.4% (many diffs)
6 mouse 19053 Ppp2cb protein phosphatase 2 (form... XM_006509033.1 72.1% 78.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
993
ORF length:
927
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga cgacaaggcg ttcaccaagg agctggacca gtgggtcgag cagctgaacg
121agtgtaagca gctgaacgag aaccaagtgc ggacgctgtg cgagaaggca aaggaaattt
181taacaaaaga atcaaatgtg caagaggttc gttgccctgt tactgtctgt ggagatgtgc
241atggtcaatt tcatgatctt atggaactct ttagaattgg tggaaaatca ccggatacaa
301actacttatt catgggtgac tatgtagaca gaggatatta ttcagtggag actgtgactc
361ttcttgtagc attaaaggtg cgttatccag aacgcattac aatattgaga ggaaatcacg
421aaagccgaca aattacccaa gtatatggct tttatgatga atgtctgcga aagtatggga
481atgccaacgt ttggaaatat tttacagatc tctttgatta tcttccactt acagctttag
541tagatGGACA GATATTCTGC CTCCATGGTG GCCTCTCTCC ATCCATAGAC ACACTGGATC
601ATATAAGAGC CCTGGATCGT TTACAGGAAG TTCCACATGA GGGCCCAATG TGTGATCTGT
661TATGGTCAGA TCCAGATGAT CGTGGTGGAT GGGGTATTTC ACCACGTGGT GCTGGCTACA
721CATTTGGACA AGACATTTCT GAAACCTTTA ACCATGCCAA TGGTCTCACA CTGGTTTCTC
781GTGCCCACCA GCTTGTAATG GAGGGATACA ATTGGTGTCA TGATCGGAAT GTGGTTACCA
841TTTTCAGTGC ACCCAATTAC TGTTATCGTT GTGGGAACCA GGCTGCTATC ATGGAATTAG
901ATGACACTTT AAAATATTCC TTCCTTCAAT TTGACCCAGC GCCTCGTCGT GGTGAGCCTC
961ATGTTACACG GCGCACCCCA GACTACTTCC TATACCCAAC TTTCTTGTAC AAAGTGGTTG
1021ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1081GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC
1141CGTGTTGCGG ATAGAGAATC TTACGCGTTA AGTCgacaat caacctctgg attacaaaat
1201ttgtgaaaga tt

Download FASTA (ORF) (Full)