Construct: ORF TRCN0000472494
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017817.1_s317c1
- Derived from:
- ccsbBroadEn_01262
- DNA Barcode:
- TCCGTGTTGCGGATAGAGAATCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP2CB (5516)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472494
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5516 | PPP2CB | protein phosphatase 2 catal... | NM_001009552.2 | 100% | 100% | |
| 2 | human | 5515 | PPP2CA | protein phosphatase 2 catal... | NM_002715.4 | 83.1% | 97.4% | (many diffs) |
| 3 | human | 5515 | PPP2CA | protein phosphatase 2 catal... | NM_001355019.1 | 65% | 78.6% | (many diffs) |
| 4 | mouse | 19053 | Ppp2cb | protein phosphatase 2 (form... | NM_017374.3 | 92.1% | 100% | (many diffs) |
| 5 | mouse | 19052 | Ppp2ca | protein phosphatase 2 (form... | NM_019411.4 | 80.9% | 97.4% | (many diffs) |
| 6 | mouse | 19053 | Ppp2cb | protein phosphatase 2 (form... | XM_006509033.1 | 72.1% | 78.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 993
- ORF length:
- 927
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgacaaggcg ttcaccaagg agctggacca gtgggtcgag cagctgaacg 121 agtgtaagca gctgaacgag aaccaagtgc ggacgctgtg cgagaaggca aaggaaattt 181 taacaaaaga atcaaatgtg caagaggttc gttgccctgt tactgtctgt ggagatgtgc 241 atggtcaatt tcatgatctt atggaactct ttagaattgg tggaaaatca ccggatacaa 301 actacttatt catgggtgac tatgtagaca gaggatatta ttcagtggag actgtgactc 361 ttcttgtagc attaaaggtg cgttatccag aacgcattac aatattgaga ggaaatcacg 421 aaagccgaca aattacccaa gtatatggct tttatgatga atgtctgcga aagtatggga 481 atgccaacgt ttggaaatat tttacagatc tctttgatta tcttccactt acagctttag 541 tagatGGACA GATATTCTGC CTCCATGGTG GCCTCTCTCC ATCCATAGAC ACACTGGATC 601 ATATAAGAGC CCTGGATCGT TTACAGGAAG TTCCACATGA GGGCCCAATG TGTGATCTGT 661 TATGGTCAGA TCCAGATGAT CGTGGTGGAT GGGGTATTTC ACCACGTGGT GCTGGCTACA 721 CATTTGGACA AGACATTTCT GAAACCTTTA ACCATGCCAA TGGTCTCACA CTGGTTTCTC 781 GTGCCCACCA GCTTGTAATG GAGGGATACA ATTGGTGTCA TGATCGGAAT GTGGTTACCA 841 TTTTCAGTGC ACCCAATTAC TGTTATCGTT GTGGGAACCA GGCTGCTATC ATGGAATTAG 901 ATGACACTTT AAAATATTCC TTCCTTCAAT TTGACCCAGC GCCTCGTCGT GGTGAGCCTC 961 ATGTTACACG GCGCACCCCA GACTACTTCC TATACCCAAC TTTCTTGTAC AAAGTGGTTG 1021 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1081 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 1141 CGTGTTGCGG ATAGAGAATC TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1201 ttgtgaaaga tt