Transcript: Human NM_001009552.2

Homo sapiens protein phosphatase 2 catalytic subunit beta (PPP2CB), mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
PPP2CB (5516)
Length:
1946
CDS:
398..1327

Additional Resources:

NCBI RefSeq record:
NM_001009552.2
NBCI Gene record:
PPP2CB (5516)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001009552.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000002506 AGCCGACAAATTACCCAAGTA pLKO.1 755 CDS 100% 4.950 6.930 N PPP2CB n/a
2 TRCN0000318551 AGCCGACAAATTACCCAAGTA pLKO_005 755 CDS 100% 4.950 6.930 N PPP2CB n/a
3 TRCN0000002507 TGTCTGCGAAAGTATGGGAAT pLKO.1 794 CDS 100% 4.050 5.670 N PPP2CB n/a
4 TRCN0000349621 TGTCTGCGAAAGTATGGGAAT pLKO_005 794 CDS 100% 4.050 5.670 N PPP2CB n/a
5 TRCN0000002505 GAAATCACGAAAGCCGACAAA pLKO.1 744 CDS 100% 4.950 3.960 N PPP2CB n/a
6 TRCN0000318493 GAAATCACGAAAGCCGACAAA pLKO_005 744 CDS 100% 4.950 3.960 N PPP2CB n/a
7 TRCN0000012415 GCTGCTATCATGGAATTAGAT pLKO.1 1214 CDS 100% 5.625 3.938 N Ppp2cb n/a
8 TRCN0000002508 AGGTTCTTCTTGGGAGTATGT pLKO.1 1782 3UTR 100% 4.950 3.465 N PPP2CB n/a
9 TRCN0000318552 AGGTTCTTCTTGGGAGTATGT pLKO_005 1782 3UTR 100% 4.950 3.465 N PPP2CB n/a
10 TRCN0000002504 GCTTTAGTAGATGGACAGATA pLKO.1 866 CDS 100% 4.950 3.465 N PPP2CB n/a
11 TRCN0000380254 TAGACACACTGGATCATATAA pLKO_005 918 CDS 100% 15.000 9.000 N PPP2CB n/a
12 TRCN0000294514 GAACGAGTGTAAGCAGCTGAA pLKO_005 448 CDS 100% 4.050 2.430 N Ppp2cb n/a
13 TRCN0000294515 TAAGCAGCTGAACGAGAACCA pLKO_005 457 CDS 100% 2.640 1.584 N Ppp2cb n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001009552.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01262 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01262 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000472494 TCCGTGTTGCGGATAGAGAATCTT pLX_317 48.3% 100% 100% V5 n/a
4 TRCN0000488986 AAGAGGAGCAGGTAGGGGCTAGTG pLX_317 32.9% 100% 100% V5 n/a
5 TRCN0000488598 GTAAACAACTCACACCTAAAGTCC pLX_317 38.4% 99.3% 100% V5 (not translated due to prior stop codon) 927_928insTAAGCT n/a
6 TRCN0000489912 GGACAAGAAATCAACTTGTATCGC pLX_317 40.1% 83.1% 97.4% V5 (many diffs) n/a
7 TRCN0000489841 CCTCCTTCCTTGGGTTCTATCTTC pLX_317 44.8% 82.6% 97.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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