Construct: ORF TRCN0000472511
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000904.1_s317c1
- Derived from:
- ccsbBroadEn_01281
- DNA Barcode:
- CAAAGCGAGGGGGAGCATCGCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKCA (5578)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472511
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5578 | PRKCA | protein kinase C alpha | NM_002737.3 | 99.9% | 99.8% | 1702G>A |
| 2 | human | 5578 | PRKCA | protein kinase C alpha | XM_017024836.2 | 93.3% | 92.5% | (many diffs) |
| 3 | human | 5578 | PRKCA | protein kinase C alpha | XM_017024837.1 | 91.3% | 89.5% | (many diffs) |
| 4 | human | 5578 | PRKCA | protein kinase C alpha | XM_024450829.1 | 86.4% | 85.8% | (many diffs) |
| 5 | human | 5578 | PRKCA | protein kinase C alpha | XM_024450830.1 | 86.4% | 85.8% | (many diffs) |
| 6 | human | 5578 | PRKCA | protein kinase C alpha | XR_001752558.1 | 55.7% | (many diffs) | |
| 7 | human | 5578 | PRKCA | protein kinase C alpha | XM_017024841.1 | 47.1% | 45.6% | (many diffs) |
| 8 | mouse | 18750 | Prkca | protein kinase C, alpha | NM_011101.3 | 91% | 99.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2085
- ORF length:
- 2016
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgacgtt ttcccgggca acgactccac ggcgtctcag gacgtggcca 121 accgcttcgc ccgcaaaggg gcgctgaggc agaagaacgt gcacgaggtg aaggaccaca 181 aattcatcgc gcgcttcttc aagcagccca ccttctgcag ccactgcacc gacttcatct 241 gggggtttgg gaaacaaggc ttccagtgcc aagtttgctg ttttgtggtc cacaagaggt 301 gccatgaatt tgttactttt tcttgtccgg gtgcggataa gggacccgac actgatgacc 361 ccaggagcaa gcacaagttc aaaatccaca cttacggaag ccccaccttc tgcgatcact 421 gtgggtcact gctctatgga cttatccatc aagggatgaa atgtgacacc tgcgatatga 481 acgttcacaa gcaatgcgtc atcaatgtcc ccagcctctg cggaatggat cacactgaga 541 agagggggcg gatttaccta aaggctgagg ttgctgatga aaagctccat gtcacagtac 601 gagatgcaaa aaatctaatc cctatggatc caaacgggct ttcagatcct tatgtgaagc 661 tgaaacttat tcctgatccc aagaatgaaa gcaagcaaaa aaccaaaacc atccgctcca 721 cactaaatcc gcagtggaat gagtccttta cattcaaatt gaaaccttca gacaaagacc 781 gacgactgtc tgtagaaatc tgggactggg atcgaacaac aaggaatgac ttcatgggat 841 ccctttcctt tggagtttcg gagctgatga agatgccggc cagtggatgg tacaagttgc 901 ttaaccaaga agaaggtgag tactacaacg tacccattcc ggaaggggac gaggaaggaa 961 acatggaact caggcagaaa ttcgagaaag ccaaacttgg ccctgctggc aacaaagtca 1021 tcagtccctc tgaagacagg aaacaacctt ccaacaacct tgaccgagtg aaactcacgg 1081 acttcaattt cctcatggtg ttgggaaagg ggagttttgg aaaggtgatg cttgccgaca 1141 ggaagggcac agaagaactg tatgcaatca aaatcctgaa gaaggatgtg gtgattcagg 1201 atgatgacgt ggagtgcacc atggtagaaa agcgagtctt ggccctgctt gacaaacccc 1261 cgttcttgac gcagctgcac tcctgcttcc agacagtgga tcggctgtac ttcgtcatgg 1321 aatatgtcaa cggtggggac ctcatgtacc acattcagca agtaggaaaa tttaaggaac 1381 cacaagcagt attctatgcg gcagagattt ccatcggatt gttctttctt cataaaagag 1441 gaatcattta tagggatctg aagttagata acgtcatgtt ggattcagaa ggacatatca 1501 aaattgctga ctttgggatg tgcaaggaac acatgatgga tggagtcacg accaggacct 1561 tctgtgggac tccagattat atcgccccag agataatcgc ttatcagccg tatggaaaat 1621 ctgtggactg gtgggccTAT GGCGTCCTGT TGTATGAAAT GCTTGCCGGG CAGCCTCCAT 1681 TTGATGGTGA AGATGAAGAC GAGCTATTTC AGTCTATCAT GGAGCACAAC GTTTCCTATC 1741 CAAAATCCTT GTCCAAGGAG GCTGTTTCTA TCTGCAAAGG ACTGATGACC AAACACCCAG 1801 CCAAGCGGCT GGGCTGTGGG CCTGAGGGGG AGAGGGACGT GAGAGAGCAT GCCTTCTTCC 1861 GGAGGATCGA CTGGGAAAAA CTGGAGAACA GGGAGATCCA GCCACCATTC AAGCCCAAAG 1921 TGTGTGGCAA AGGAGCAGAG AACTTTGACA AGTTCTTCAC ACGAGGACAG CCCGTCTTAA 1981 CACCACCTGA TCAGCTGGTT ATTGCTAACA TAGACCAGTC TGATTTTGAA GGGTTCTCGT 2041 ATGTCAACCC CCAGTTTGTG CACCCCATCT TACAGAGTGC AGTATTGCCA ACTTTCTTGT 2101 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2161 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2221 GAAAGGACGA CAAAGCGAGG GGGAGCATCG CTTCACGCGT TAAGTCgaca atcaacctct 2281 ggattacaaa atttgtgaaa gatt