Construct: ORF TRCN0000472539
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001022.1_s317c1
- Derived from:
- ccsbBroadEn_14378
- DNA Barcode:
- TCCTATATCCCGTTATGGATGAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC148 (130940)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472539
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 130940 | CCDC148 | coiled-coil domain containi... | NM_001301684.1 | 98.3% | 97.9% | (many diffs) |
| 2 | human | 130940 | CCDC148 | coiled-coil domain containi... | XM_017003397.1 | 98.3% | 97.9% | (many diffs) |
| 3 | human | 130940 | CCDC148 | coiled-coil domain containi... | XM_017003396.1 | 86.6% | 84.7% | (many diffs) |
| 4 | human | 130940 | CCDC148 | coiled-coil domain containi... | XM_024452710.1 | 86.6% | 84.7% | (many diffs) |
| 5 | human | 130940 | CCDC148 | coiled-coil domain containi... | XM_017003395.1 | 84.4% | 82.6% | (many diffs) |
| 6 | human | 130940 | CCDC148 | coiled-coil domain containi... | XM_017003394.1 | 79.4% | 77.6% | (many diffs) |
| 7 | human | 130940 | CCDC148 | coiled-coil domain containi... | XM_017003398.1 | 78% | 74.7% | (many diffs) |
| 8 | human | 130940 | CCDC148 | coiled-coil domain containi... | NM_138803.4 | 74% | 72.4% | (many diffs) |
| 9 | human | 130940 | CCDC148 | coiled-coil domain containi... | XM_017003392.1 | 72.4% | 70.8% | (many diffs) |
| 10 | human | 130940 | CCDC148 | coiled-coil domain containi... | XM_005246319.3 | 71.5% | 69.9% | (many diffs) |
| 11 | human | 130940 | CCDC148 | coiled-coil domain containi... | XM_011510638.3 | 70% | 68.4% | (many diffs) |
| 12 | human | 130940 | CCDC148 | coiled-coil domain containi... | NM_001301685.1 | 17.3% | 16% | 1_304del;334_485del;765_765delGins1009 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1386
- ORF length:
- 1317
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaatgtcttt gtgaccttac aaattttggt tgactttgtg aagaaacaat 121 tgaaaactgt ctttgaaaga cttaggctgg agcaacagag aatagaaaat gatctttcag 181 actggagtat aaagattcta gatcattctt tggaagaaaa gactaacccg cttagtgaac 241 tgcccattga attagaaagt ttggaatgcc cataccctga tttgaaatct tcaattctca 301 gtgagttcta taagtttaca cagaaatatc agaagaagct tcaagacttt aatctgcagt 361 tggaagacat atacagaaac tgtcaactaa gtgaagaaga ccactggatt taccaggcta 421 ttttggatca gtaccctgga gatctctttg gaagaaggac tctgtatctg gacatgttac 481 aaagatattt tcctcacaaa tctaggcatg atttggttga acacgagaaa tattgtgacc 541 aatatcgctt tgctatagag cagcaaaata tcctgatatc aaattggaat aaaaatagga 601 aagactttat acagaaggct gtgctgacac tcactgaggc ttgtgcaaca catgaaatgg 661 agagcatgtt ggctaaggac aagaaaaagc aacaggaatt gtgtgctgat ctgaaagcca 721 aggttcgtca atggcgagcc caccaagaag aggtagcaag actggaaatg gaaatttctg 781 ccagaagaag agaaaaggaa gaggagaaag agaaactgtg gaagaagaag gaattgttgc 841 aaagagcaga gaagaaaaaa agcataaaaa aatactgggc caagaaaaaa cagaagtggc 901 aagaaatGGA AATGAGAGAT CTTCAGCGTC TAGAAGAACT GAAGAAATTA ATCGCTGAAC 961 AGTCACTAAA AGACAGAGAA AGGGTTAAAT ATAGACAAGA ATTATTGGAA AGGCGTTTGA 1021 TGGAGAAAAA GGAAGTGGCC CTTCAAGAAG CTCATGAAGA CAAAGAGAGA GCACGGCGGC 1081 TAGAAGCCCT TAGGAAACAG GTTGCTGTTG TTGCTCAATT TGATCCTGTT AGAATGATGT 1141 CAGATACAAT GGCATCAAAA GCTAGAATGG GCATTGAAAT TGAAGAAGAA TTTATTCTTC 1201 AAAAGCCACT CTTCACATTG AATACATACA ATGAACAGCA GATAATTTCT GACCCTAGAC 1261 TTCGCTTCGA GTTAGCACTT CGAGAAGCTG GACTTCATAG AACACTTTAT GCTAAAGAGA 1321 TATTACCAAA AATTAGTCCT CAAAAACCTC CAAGAAAGGA CATGGAGTCT ACTGTATTGA 1381 AAATATTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1441 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1501 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCCTATATC CCGTTATGGA TGAATACGCG 1561 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt