Construct: ORF TRCN0000472554
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012598.1_s317c1
- Derived from:
- ccsbBroadEn_14123
- DNA Barcode:
- GTTACTACCGTATGTCTCAGGCAA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDCD4 (27250)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472554
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27250 | PDCD4 | programmed cell death 4 | NM_014456.5 | 99.7% | 98.9% | 106A>G;143C>A;1400delA |
| 2 | human | 27250 | PDCD4 | programmed cell death 4 | NM_145341.3 | 97.2% | 95.7% | (many diffs) |
| 3 | human | 27250 | PDCD4 | programmed cell death 4 | NM_001199492.1 | 96.8% | 95.9% | (many diffs) |
| 4 | mouse | 18569 | Pdcd4 | programmed cell death 4 | NM_001168491.1 | 89.3% | 95.7% | (many diffs) |
| 5 | mouse | 18569 | Pdcd4 | programmed cell death 4 | NM_001168492.1 | 89.3% | 95.7% | (many diffs) |
| 6 | mouse | 18569 | Pdcd4 | programmed cell death 4 | NM_011050.4 | 89.3% | 95.7% | (many diffs) |
| 7 | mouse | 18569 | Pdcd4 | programmed cell death 4 | XM_006526762.3 | 89.3% | 95.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1473
- ORF length:
- 1407
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgtagaaaat gagcagatac tgaatgtaaa ccctgcagat cctgataact 121 taagtgactc tctcttttcc ggtgatgaag aaaatgctgg gactgaggaa gtaaagaatg 181 aaataaatgg aaattggatt tcagcatact ccattaacga agctagaatt aatgccaagg 241 caaaaaggcg actaaggaaa aactcatccc gggactctgg cagaggcgat tcggtcagcg 301 acagtgggag tgacgccctt agaagtggat taactgtgcc aaccagtcca aagggaaggt 361 tgctggatag gcgatccaga tctgggaaag gaaggggact accaaagaaa ggtggtgcag 421 gaggcaaagg tgtctggggt acacctggac aggtgtatga tgtggaggag gtggatgtga 481 aagatcctaa ctatgatgat gaccaggaga actgtgttta tgaaactgta gttttgcctt 541 tggatgaaag ggcatttgag aagactttaa caccaatcat acaggaatat tttgagcatg 601 gagatactaa tgaagttgcg gaaatgttaa gagatttaaa tcttggtgaa atgaaaagtg 661 gagtaccagt gttggcagta tccttagcat tggaggggaa ggctagtcat agagagatga 721 catctaagct tctttctgac ctttgtggga cagtaatgag cacaactgat gtggaaaaat 781 catttgataa attgttgaaa gatctacctg aattagcact ggatactcct agagcaccac 841 agttggtggg ccagtttatt gctagagctg ttggagatgg aattttatgt aatacctata 901 ttgatagtta caaaggaact gtagattgtg tgcaggctag agctgctctg gataaggcta 961 ccgtgcttct gagtatgtct aaaggtggaa agcgtaaaga tagtgtgtgg ggctctggag 1021 gtgggcagca aTCTGTCAAT CACCTTGTTA AAGAGATTGA TATGCTGCTG AAAGAATATT 1081 TACTCTCTGG AGACATATCT GAAGCTGAAC ATTGCCTTAA GGAACTGGAA GTACCTCATT 1141 TTCACCATGA GCTTGTATAT GAAGCTATTA TAATGGTTTT AGAGTCAACT GGAGAAAGTA 1201 CATTTAAGAT GATTTTGGAT TTATTAAAGT CCCTTTGGAA GTCTTCTACC ATTACTGTAG 1261 ACCAAATGAA AAGAGGTTAT GAGAGAATTT ACAATGAAAT TCCGGACATT AATCTGGATG 1321 TCCCACATTC ATACTCTGTG CTGGAGCGGT TTGTAGAAGA ATGTTTTCAG GCTGGAATAA 1381 TTTCCAAACA ACTCAGAGAT CTTTGTCCTT CAAGGGGCAG AAAGCGTTTT GTAAGCGAAG 1441 GAGATGGAGG TCGTCTTAAA CCAGGAGCTA CTGCCCAACT TTCTTGTACA AAGTGGTTGA 1501 TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG 1561 TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTT 1621 ACTACCGTAT GTCTCAGGCA AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 1681 tgtgaaagat t