Construct: ORF TRCN0000472584

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007540.1_s317c1
Derived from:
ccsbBroadEn_13795
DNA Barcode:
TCCGTTTATTTAGTTGATAAGTTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472584
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 100174949 TRIM59-IFT80 TRIM59-IFT80 protein NR_148401.1 83.9% (many diffs)
2 human 57560 IFT80 intraflagellar transport 80 NM_020800.3 74.6% 67.2% (many diffs)
3 human 57560 IFT80 intraflagellar transport 80 NM_001190241.2 66.7% 66.5% (many diffs)
4 human 57560 IFT80 intraflagellar transport 80 NM_001190242.1 66.7% 66.5% (many diffs)
5 human 100174949 TRIM59-IFT80 TRIM59-IFT80 protein NR_148402.1 46.4% (many diffs)
6 human 100174949 TRIM59-IFT80 TRIM59-IFT80 protein NR_148403.1 44.5% (many diffs)
7 human 286827 TRIM59 tripartite motif containing 59 NM_173084.3 39.6% 34.1% (many diffs)
8 mouse 68259 Ift80 intraflagellar transport 80 NM_026641.2 68.5% 61.1% (many diffs)
9 mouse 68259 Ift80 intraflagellar transport 80 XM_006501966.3 68.5% 61.1% (many diffs)
10 mouse 68259 Ift80 intraflagellar transport 80 XM_017319702.1 66.1% 58.7% (many diffs)
11 mouse 68259 Ift80 intraflagellar transport 80 XM_006501967.3 63% 55.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2910
ORF length:
2844
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca caattttgag gaagagttaa cttgtcccat atgttatagt atttttgaag
121atcctcgtgt actgccatgc tctcatacat tttgtagaaa ttgtttggaa aacattcttc
181aggcatctgg taacttttat atatggagac ctttacgaat tccactcaag tgccctaatt
241gcagaagtat tactgaaatt gctccaactg gcattgaatc tttacctgtt aattttgcac
301taagggctat tattgaaaag taccagcaag aagaccatcc agatattgtc acctgccctg
361aacattacag gcaaccatta aatgtttact gtctattaga taaaaaatta gtttgtggtc
421attgccttac cataggtcaa catcatggtc atcctataga tgaccttcaa agtgcctatt
481tgaaagaaaa ggacactcct caaaaactgc ttgaacagtt gactgacaca cactggacag
541atcttaccca tcttattgaa aagccgaaag aacaaaaatc tcattctgag aaaatgatcc
601aaggcgataa ggaagctgtt ctccagtatt ttaaggagct taatgataca ttagaacaga
661aaaaaaaaag tttcctaacg gctctctgtg atgttggcaa tctaattaat caagaatata
721ctccacaaat tgaaagaatg aaggaaatac gagagcagca gcttgaatta atggcactga
781caatatcttt acaagaagag tctccactta aatttcttga aaaagttgat gatgtacgcc
841agcatgtaca gatcttgaaa caaagaccac ttcctgaggt tcaacccgtt gaaatttatc
901ctcgagtaag caaaatattg aaagaagaat ggagcagaac agaaattgga caaattaaga
961acgttctcat tcccaaaatg aaaatttctc caaaaaggat gtcatgttcc tggcctggaa
1021caccagtgta ttcagtagcg tggggccctg attcagaaaa ggttctttat acagcaggca
1081agcagctaat cattaaacct cttcaaccaa atgctaaagt tttgcagtgg aaagctcatg
1141atggcattat tttaaaagta gattggaact cggtcaatga tcttatttta tctgctggtg
1201aagactgtaa atataaggta tgggatagtt acggccgccc actgtacaat tcacaacctc
1261atgagcatcc cattacttca gttgcctggg ctccagatgg agaattattt gctgttggat
1321cgtttcatac tttacgcttg tgtgataaaa ctgggtggtc atatgcatta gaaaaaccca
1381acactggcag catatttaat attgcatggt ctatcgatgg cactcagatt gctggagcct
1441gtggaaatgg acatgtcgtt tttgcacatg tggtggaaca acattgggag tggaaaaatt
1501ttcaagtaac attaacgaaa agaagagcca tgcaggttcg taatgttctt aatgatgcag
1561tggatttact ggaattccgt gatagagtca ttaaagcatc tttgaactat gcacacttag
1621ttgtttcaac gtctcttcaa tgttacgtgt tctccacgaa gaactggaac acaccaatta
1681tatttgatct caaagaagga actgttagtt tgattctgca ggcagaaaga cattttcttc
1741ttgtagatgg tagtagtatc tatttatatt catatgaagg gcgctttatt tcatctccaa
1801aatttcctgg aatgagaaca gatattctga atgcacagac tgtgtctttg agtaatgata
1861ccatagcaat aagagacaaa gctgatgaaa aaataatctt cctctttgag gcatcaaccg
1921gaaagccgtt aggtgatgga aagtttcttt ctcataagaa tgaaatcttg gaaattgctc
1981tggatcaaaa aggacttacc aatgatagaa aaattgcttt cattgataaa aatagagatc
2041tctgtatcac ttctgtgaaa cgatttggga aggaagaaca aattatcaag cttggaacaa
2101tggtgcatac tttggcatgg aacgatacat gcaatatcct ttgtggactt caagatactc
2161gatttatagt gtggtattac cccaatacag tttatgtgga cagagacatt ttgcctaaaa
2221cattatatga aagggatgca agtgaattta gtaaaaatcc ccatattgtg agttttgttg
2281gaaatcaagt aactattaga agagctgatg gctccctggt tcacatcagc ataacaccat
2341atcctgctat tctccatgaa tatgtaagca gttcaaaatg ggaagatgct gtgagacttt
2401gtcgctttgt taaggagcaa accatgtggg cttgtctagc tgctatggca gttgctaatc
2461gagatatgac tactgcagaa gtagcctatg cagcaattgg tgaaattgat aaggttcagt
2521acatcaattc tataaaaaaT CTTCCATCTA AAGAATCAAA AATGGCCCAC ATACTACTGT
2581TTAGTGGGAA CATACAGGAG GCTGAAATAG TACTTCTTCA GGCTGGCCTT GTTTATCAAG
2641CAATCCAGAT CAATATTAAT CTCTACAACT GGGAAAGGGC ACTGGAATTG GCTGTAAAAT
2701ACAAAACACA TGTTGATACA GTTCTTGCTT ACCGTCAAAA GTTTTTGGAG ACATTTGGTA
2761AACAGGAAAC TAATAAACGA TACTTGCATT ATGCAGAAGG TCTCCAAATA GATTGGGAGA
2821AAATCAAAGC CAAAATTGAG ATGGAAATTA CAAAAGAAAG AGAGCAATCA TCAAGCAGCC
2881AATCCAGCAA GAGTATAGGT TTAAAGCCCT ACCCAACTTT CTTGTACAAA GTGGTTGATA
2941TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
3001CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCCGT
3061TTATTTAGTT GATAAGTTGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
3121tgaaagatt

Download FASTA (ORF) (Full)