Construct: ORF TRCN0000472584
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007540.1_s317c1
- Derived from:
- ccsbBroadEn_13795
- DNA Barcode:
- TCCGTTTATTTAGTTGATAAGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472584
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100174949 | TRIM59-IFT80 | TRIM59-IFT80 protein | NR_148401.1 | 83.9% | (many diffs) | |
| 2 | human | 57560 | IFT80 | intraflagellar transport 80 | NM_020800.3 | 74.6% | 67.2% | (many diffs) |
| 3 | human | 57560 | IFT80 | intraflagellar transport 80 | NM_001190241.2 | 66.7% | 66.5% | (many diffs) |
| 4 | human | 57560 | IFT80 | intraflagellar transport 80 | NM_001190242.1 | 66.7% | 66.5% | (many diffs) |
| 5 | human | 100174949 | TRIM59-IFT80 | TRIM59-IFT80 protein | NR_148402.1 | 46.4% | (many diffs) | |
| 6 | human | 100174949 | TRIM59-IFT80 | TRIM59-IFT80 protein | NR_148403.1 | 44.5% | (many diffs) | |
| 7 | human | 286827 | TRIM59 | tripartite motif containing 59 | NM_173084.3 | 39.6% | 34.1% | (many diffs) |
| 8 | mouse | 68259 | Ift80 | intraflagellar transport 80 | NM_026641.2 | 68.5% | 61.1% | (many diffs) |
| 9 | mouse | 68259 | Ift80 | intraflagellar transport 80 | XM_006501966.3 | 68.5% | 61.1% | (many diffs) |
| 10 | mouse | 68259 | Ift80 | intraflagellar transport 80 | XM_017319702.1 | 66.1% | 58.7% | (many diffs) |
| 11 | mouse | 68259 | Ift80 | intraflagellar transport 80 | XM_006501967.3 | 63% | 55.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2910
- ORF length:
- 2844
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca caattttgag gaagagttaa cttgtcccat atgttatagt atttttgaag 121 atcctcgtgt actgccatgc tctcatacat tttgtagaaa ttgtttggaa aacattcttc 181 aggcatctgg taacttttat atatggagac ctttacgaat tccactcaag tgccctaatt 241 gcagaagtat tactgaaatt gctccaactg gcattgaatc tttacctgtt aattttgcac 301 taagggctat tattgaaaag taccagcaag aagaccatcc agatattgtc acctgccctg 361 aacattacag gcaaccatta aatgtttact gtctattaga taaaaaatta gtttgtggtc 421 attgccttac cataggtcaa catcatggtc atcctataga tgaccttcaa agtgcctatt 481 tgaaagaaaa ggacactcct caaaaactgc ttgaacagtt gactgacaca cactggacag 541 atcttaccca tcttattgaa aagccgaaag aacaaaaatc tcattctgag aaaatgatcc 601 aaggcgataa ggaagctgtt ctccagtatt ttaaggagct taatgataca ttagaacaga 661 aaaaaaaaag tttcctaacg gctctctgtg atgttggcaa tctaattaat caagaatata 721 ctccacaaat tgaaagaatg aaggaaatac gagagcagca gcttgaatta atggcactga 781 caatatcttt acaagaagag tctccactta aatttcttga aaaagttgat gatgtacgcc 841 agcatgtaca gatcttgaaa caaagaccac ttcctgaggt tcaacccgtt gaaatttatc 901 ctcgagtaag caaaatattg aaagaagaat ggagcagaac agaaattgga caaattaaga 961 acgttctcat tcccaaaatg aaaatttctc caaaaaggat gtcatgttcc tggcctggaa 1021 caccagtgta ttcagtagcg tggggccctg attcagaaaa ggttctttat acagcaggca 1081 agcagctaat cattaaacct cttcaaccaa atgctaaagt tttgcagtgg aaagctcatg 1141 atggcattat tttaaaagta gattggaact cggtcaatga tcttatttta tctgctggtg 1201 aagactgtaa atataaggta tgggatagtt acggccgccc actgtacaat tcacaacctc 1261 atgagcatcc cattacttca gttgcctggg ctccagatgg agaattattt gctgttggat 1321 cgtttcatac tttacgcttg tgtgataaaa ctgggtggtc atatgcatta gaaaaaccca 1381 acactggcag catatttaat attgcatggt ctatcgatgg cactcagatt gctggagcct 1441 gtggaaatgg acatgtcgtt tttgcacatg tggtggaaca acattgggag tggaaaaatt 1501 ttcaagtaac attaacgaaa agaagagcca tgcaggttcg taatgttctt aatgatgcag 1561 tggatttact ggaattccgt gatagagtca ttaaagcatc tttgaactat gcacacttag 1621 ttgtttcaac gtctcttcaa tgttacgtgt tctccacgaa gaactggaac acaccaatta 1681 tatttgatct caaagaagga actgttagtt tgattctgca ggcagaaaga cattttcttc 1741 ttgtagatgg tagtagtatc tatttatatt catatgaagg gcgctttatt tcatctccaa 1801 aatttcctgg aatgagaaca gatattctga atgcacagac tgtgtctttg agtaatgata 1861 ccatagcaat aagagacaaa gctgatgaaa aaataatctt cctctttgag gcatcaaccg 1921 gaaagccgtt aggtgatgga aagtttcttt ctcataagaa tgaaatcttg gaaattgctc 1981 tggatcaaaa aggacttacc aatgatagaa aaattgcttt cattgataaa aatagagatc 2041 tctgtatcac ttctgtgaaa cgatttggga aggaagaaca aattatcaag cttggaacaa 2101 tggtgcatac tttggcatgg aacgatacat gcaatatcct ttgtggactt caagatactc 2161 gatttatagt gtggtattac cccaatacag tttatgtgga cagagacatt ttgcctaaaa 2221 cattatatga aagggatgca agtgaattta gtaaaaatcc ccatattgtg agttttgttg 2281 gaaatcaagt aactattaga agagctgatg gctccctggt tcacatcagc ataacaccat 2341 atcctgctat tctccatgaa tatgtaagca gttcaaaatg ggaagatgct gtgagacttt 2401 gtcgctttgt taaggagcaa accatgtggg cttgtctagc tgctatggca gttgctaatc 2461 gagatatgac tactgcagaa gtagcctatg cagcaattgg tgaaattgat aaggttcagt 2521 acatcaattc tataaaaaaT CTTCCATCTA AAGAATCAAA AATGGCCCAC ATACTACTGT 2581 TTAGTGGGAA CATACAGGAG GCTGAAATAG TACTTCTTCA GGCTGGCCTT GTTTATCAAG 2641 CAATCCAGAT CAATATTAAT CTCTACAACT GGGAAAGGGC ACTGGAATTG GCTGTAAAAT 2701 ACAAAACACA TGTTGATACA GTTCTTGCTT ACCGTCAAAA GTTTTTGGAG ACATTTGGTA 2761 AACAGGAAAC TAATAAACGA TACTTGCATT ATGCAGAAGG TCTCCAAATA GATTGGGAGA 2821 AAATCAAAGC CAAAATTGAG ATGGAAATTA CAAAAGAAAG AGAGCAATCA TCAAGCAGCC 2881 AATCCAGCAA GAGTATAGGT TTAAAGCCCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 2941 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 3001 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCCGT 3061 TTATTTAGTT GATAAGTTGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 3121 tgaaagatt