Transcript: Human NM_001190241.2

Homo sapiens intraflagellar transport 80 (IFT80), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
IFT80 (57560)
Length:
4219
CDS:
758..2680

Additional Resources:

NCBI RefSeq record:
NM_001190241.2
NBCI Gene record:
IFT80 (57560)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001190241.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000133680 GCTGGTGAAGACTGTAAATAT pLKO.1 962 CDS 100% 15.000 7.500 Y IFT80 n/a
2 TRCN0000137720 GCAGGCAAGCAGCTAATCATT pLKO.1 842 CDS 100% 5.625 2.813 Y IFT80 n/a
3 TRCN0000193055 CCAAGTTAGGAAGAGTGGAAA pLKO.1 624 5UTR 100% 4.950 2.475 Y Ift80 n/a
4 TRCN0000345148 CCAAGTTAGGAAGAGTGGAAA pLKO_005 624 5UTR 100% 4.950 2.475 Y Ift80 n/a
5 TRCN0000134272 GCAGTGTACTTACAAGTTGTA pLKO.1 4063 3UTR 100% 4.950 2.475 Y IFT80 n/a
6 TRCN0000134706 GCGCTTTATTTCATCTCCAAA pLKO.1 1549 CDS 100% 4.950 2.475 Y IFT80 n/a
7 TRCN0000137906 GCTGTGAGACTTTGTCGCTTT pLKO.1 2156 CDS 100% 4.050 2.025 Y IFT80 n/a
8 TRCN0000135575 GCAGCATTACTGGAATAGCAA pLKO.1 3160 3UTR 100% 3.000 1.500 Y IFT80 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001190241.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03831 pDONR223 100% 82.3% 82.3% None 0_1ins411 n/a
2 ccsbBroad304_03831 pLX_304 0% 82.3% 82.3% V5 0_1ins411 n/a
3 TRCN0000465441 CCTGACTAGTTGCCCATCCCATAG pLX_317 16.1% 82.3% 82.3% V5 0_1ins411 n/a
4 ccsbBroadEn_13795 pDONR223 100% 66.7% 66.5% None (many diffs) n/a
5 ccsbBroad304_13795 pLX_304 0% 66.7% 66.5% V5 (many diffs) n/a
6 TRCN0000472584 TCCGTTTATTTAGTTGATAAGTTG pLX_317 13.3% 66.7% 66.5% V5 (many diffs) n/a
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