Construct: ORF TRCN0000472585
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001383.1_s317c1
- Derived from:
- ccsbBroadEn_03024
- DNA Barcode:
- CGCTCACCAGCAGTCCTTAGGCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF544 (27300)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472585
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320767.1 | 100% | 100% | |
| 2 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320769.1 | 100% | 100% | |
| 3 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_014480.3 | 100% | 100% | |
| 4 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320770.1 | 96% | 96% | 159_160ins84 |
| 5 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320771.1 | 96% | 96% | 159_160ins84 |
| 6 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320773.1 | 96% | 96% | 159_160ins84 |
| 7 | human | 27300 | ZNF544 | zinc finger protein 544 | XM_011526738.2 | 92.9% | 92.9% | 1_162del |
| 8 | human | 27300 | ZNF544 | zinc finger protein 544 | XM_024451453.1 | 80.1% | 80.1% | 0_1ins426 |
| 9 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320774.2 | 67.1% | 67.1% | 242_243ins705 |
| 10 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320780.2 | 16.1% | 12.5% | (many diffs) |
| 11 | human | 27300 | ZNF544 | zinc finger protein 544 | XM_017026599.1 | 15% | 11.6% | (many diffs) |
| 12 | human | 27300 | ZNF544 | zinc finger protein 544 | XM_017026600.1 | 13.5% | 8.4% | (many diffs) |
| 13 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320782.1 | 13% | 11.4% | (many diffs) |
| 14 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320783.2 | 12.5% | 7.9% | (many diffs) |
| 15 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320776.2 | 11.6% | 7.4% | (many diffs) |
| 16 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320788.1 | 10.4% | 7.7% | 159_160ins35;225_226ins1885 |
| 17 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320789.1 | 10.4% | 7.7% | 159_160ins35;225_226ins1885 |
| 18 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320791.1 | 10.4% | 7.7% | 159_160ins35;225_226ins1885 |
| 19 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320792.1 | 10.4% | 7.7% | 159_160ins35;225_226ins1885 |
| 20 | human | 27300 | ZNF544 | zinc finger protein 544 | XM_017026601.1 | 10.4% | 7.1% | (many diffs) |
| 21 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320785.2 | 10.4% | 8% | (many diffs) |
| 22 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320786.2 | 10.4% | 8% | (many diffs) |
| 23 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320787.2 | 10.4% | 8% | (many diffs) |
| 24 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320781.1 | 9.7% | 7.2% | 1_162del;321_322ins35;387_388ins1885 |
| 25 | human | 27300 | ZNF544 | zinc finger protein 544 | XM_017026603.1 | 9.7% | 7.2% | 1_162del;321_322ins35;387_388ins1885 |
| 26 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320777.2 | 9.7% | 7.4% | (many diffs) |
| 27 | human | 27300 | ZNF544 | zinc finger protein 544 | XM_017026602.1 | 9.5% | 7.3% | (many diffs) |
| 28 | human | 27300 | ZNF544 | zinc finger protein 544 | XM_017026604.1 | 9.3% | 7.1% | (many diffs) |
| 29 | human | 27300 | ZNF544 | zinc finger protein 544 | XM_017026605.1 | 9% | 6.9% | 1_162del;321_322ins35;372_372delGins1901 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2211
- ORF length:
- 2145
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agcacgttct atgctggttc caccccaggc atctgtgtgc ttcgaggatg 121 tggctatggc attcacacag gaggagtggg aacagctgga cctggcccag aggacactgt 181 accgagaggt gacactggag acctgggagc atattgtctc cctggggctt ttcctttcca 241 aatctgatgt gatctctcag ctggagcaag aagaggacct gtgcagggca gagcaggagg 301 ccccccgaga ctggaaagct acccttgagg agaataggtt gaattctgaa aaagatcgag 361 ctagggaaga actatcccac cacgtggaag tgtacaggag tggaccggag gagccaccct 421 ctttggtatt aggaaaagtg caagatcaga gcaaccagtt aagggaacac caggagaact 481 ccttgaggtt catggtactc acctcagaga gactgtttgc tcaaagggaa cattgtgagc 541 ttgaacttgg gggaggttat tctctacctt ctactttaag ccttctacct acaacattac 601 ctacaagtac aggtttccct aagcccaact cacaagttaa agagttgaaa caaaattcag 661 ctttcattaa tcatgagaaa aatggagcag atgggaagca ctgtgagagt catcagtgtg 721 ctagagcttt ctgtcagagt atttacttga gtaaacttgg aaacgttgaa acaggaaaga 781 aaaaccctta tgaatatatt gtcagtggtg actctctcaa ctatggttcc tccctttgtt 841 ttcatggtag aactttttca gtgaagaaaa gtgatgactg taaggattat ggaaacctct 901 tcagtcacag tgtgtctctg aatgaacaga agccagtgca ttttgggaaa agtcagtatg 961 agtgtgatga gtgcagggaa acctgttctg agagtctgtg ccttgtacaa acagaaagaa 1021 gtggccctgg agagaccccc ttcagatgtg aggaacgctg tgctgccttc cccatggcct 1081 catctttttc tgactgtaac atcattcaga ctacagagaa gccatctgtg tgtaatcagt 1141 gtggaaaatc tttcagctgt tgtaagctca tacaccagag aacacacact ggagaaaagc 1201 ccttcgaatg tactcagtgt gggaaatctt ttagccagag ctatgacctt gtcatacatc 1261 agaggacaca cactggagag aagccctatg agtgtgacct gtgtgggaaa tccttcaccc 1321 agagatccaa acttattaca catcagcgaa ttcacactgg agaaaaaccg tatcagtgta 1381 ttgaatgcag aaaatccttc aggtggaact ctaacctcat tgtacatcag agaattcata 1441 ctggagagaa accgtatgag tgcactcact gtggaaagtc cttcagccaa agctatgagt 1501 tagttacaca taaaagaacg cacactggag aaaaaccctt caaatgtact cagtgtggga 1561 aatctttcag ccagaagtat gaccttgttg tacatcagag gacacacact ggagagaagc 1621 cctatgagtg caacctgtgt gggaaatcct tctcccagag ttccaaactt attacgcatc 1681 agcgaattca cactggagaa aaaccgtatc agtgtattga atgtgggaaa tccttcagat 1741 ggaactctaa cctcgtcata catcagagaa ttcatactgg agagaaaccg tacgattgca 1801 ctcactgtgg aaagtccttc agccaaAGCT ATCAGTTAGT TGCACATAAA AGAACTCACA 1861 CTGGAGAAAA GCCCTATGAA TGTAACGAGT GTGGAAAAGC CTTCAATCGA AGCACTCAGC 1921 TCATCAGGCA TCTGCAAATT CACACTGGGG AGAAGCCGTA CAAATGCAAT CAGTGCAATA 1981 AAGCCTTTGC AAGGAGCTCC TACCTTGTGA TGCATCAGAG AACTCACACT GGTGAGAAAC 2041 CTTTTGAGTG TAGTCAGTGT GGGAAAGCCT TTTCAGGGAG CTCTAACCTT CTTTCCCATC 2101 ACAGAATTCA TTCTGGAGAG AAACCCTATG AATGTAGTGA CTGTGGGAAA TCCTTCCGGC 2161 AGCAATCTCA ACTTGTAGTG CATCGGCGGA CACATACTGG AGAGAAACCT TACCCAACTT 2221 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2281 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2341 CTTGTGGAAA GGACGACGCT CACCAGCAGT CCTTAGGCTT ACGCGTTAAG TCgacaatca 2401 acctctggat tacaaaattt gtgaaagatt