Construct: ORF TRCN0000472684
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013086.1_s317c1
- Derived from:
- ccsbBroadEn_11782
- DNA Barcode:
- TAAATGCATCCAACAAAAACATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTHFD1L (25902)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472684
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NM_001350487.1 | 84.4% | 84.4% | 1_405del;2166C>G |
| 2 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NM_001350492.1 | 84.3% | 84.3% | 1_408del;2169C>G |
| 3 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NM_001350493.1 | 84.3% | 84.3% | 1_408del;2169C>G |
| 4 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_011535734.2 | 81.6% | 79.4% | (many diffs) |
| 5 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_017010705.1 | 81.6% | 79.4% | (many diffs) |
| 6 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_011535732.2 | 81.5% | 79.3% | (many diffs) |
| 7 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_011535733.2 | 81.5% | 79.3% | (many diffs) |
| 8 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NM_001350486.1 | 80.3% | 80.3% | 1_537del;2298C>G |
| 9 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NM_001242768.1 | 80.2% | 80.2% | 1_540del;2301C>G |
| 10 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_011535731.2 | 77.7% | 75.7% | (many diffs) |
| 11 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_024446395.1 | 77.6% | 77.7% | 1_630del;2391C>G |
| 12 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_011535730.2 | 77.6% | 75.6% | (many diffs) |
| 13 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NM_015440.4 | 74.9% | 74.9% | 1_735del;2496C>G |
| 14 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NM_001242767.1 | 74.8% | 74.8% | 1_738del;2499C>G |
| 15 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_017010702.2 | 74.8% | 74.8% | 1_738del;2499C>G |
| 16 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_017010703.2 | 74.8% | 74.8% | 1_738del;2499C>G |
| 17 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_005266907.5 | 72.6% | 70.7% | (many diffs) |
| 18 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_011535729.3 | 71.9% | 72% | (many diffs) |
| 19 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XR_001743322.2 | 65.2% | (many diffs) | |
| 20 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NR_146719.1 | 61.4% | (many diffs) | |
| 21 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XR_002956274.1 | 61% | (many diffs) | |
| 22 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NR_146720.1 | 49.9% | (many diffs) | |
| 23 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_011535737.3 | 47.5% | 47.2% | (many diffs) |
| 24 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_011535738.3 | 47.3% | 47.2% | 1_738del;2128_2129insTGACCGAA;2130_2131ins799 |
| 25 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NM_001350490.1 | 36.7% | 36.6% | 1_1delAins1390;372C>G |
| 26 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_005266911.5 | 36.5% | 36.4% | (many diffs) |
| 27 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_024446396.1 | 18.8% | 17.8% | (many diffs) |
| 28 | human | 101929583 | LOC101929583 | methylenetetrahydrofolate d... | NR_136299.1 | 15.4% | (many diffs) | |
| 29 | mouse | 270685 | Mthfd1l | methylenetetrahydrofolate d... | NM_001170785.1 | 64.5% | 69.6% | (many diffs) |
| 30 | mouse | 270685 | Mthfd1l | methylenetetrahydrofolate d... | NM_001170786.1 | 64.5% | 69.6% | (many diffs) |
| 31 | mouse | 270685 | Mthfd1l | methylenetetrahydrofolate d... | NM_172308.4 | 64.5% | 69.6% | (many diffs) |
| 32 | mouse | 270685 | Mthfd1l | methylenetetrahydrofolate d... | XM_006512449.3 | 41.5% | 43.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2265
- ORF length:
- 2199
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac aatgagcatc cagtggaaaa cacgccagct tcaaagcaag cttcacgagg 121 ctgacattgt ggtcctaggc tcacctaagc cagaagagat tccccttact tggatacaac 181 caggaactac tgttctcaac tgctcccatg acttcctgtc agggaaggtt gggtgtggct 241 ctccaagaat acattttggt ggactcattg aggaagatga tgtgattctc cttgctgcag 301 ctctgcgaat tcagaacatg gtcagtagtg gaaggagatg gcttcgtgaa cagcagcaca 361 ggcggtggag acttcactgc ttgaaacttc agcctctctc ccctgtgcca agtgacattg 421 agatttcaag aggacaaact ccaaaagctg tggatgtcct tgccaaggag attggattgc 481 ttgcagatga aattgaaatc tatggcaaaa gcaaagccaa agtacgtttg tccgtgctag 541 aaaggttaaa ggatcaagca gatggaaaat acgtcttagt tgctgggatc acacccaccc 601 ctcttggaga agggaagagc acagtcacca tcgggcttgt gcaggctctg accgcacacc 661 tgaatgtcaa ctcctttgcc tgcttgaggc agccttccca aggaccgacg tttggagtga 721 aaggaggagc cgcgggtggt ggatatgccc aggtcatccc catggaggag ttcaaccttc 781 acttgactgg agacatccac gccatcaccg ctgccaataa cttgctggct gccgccatcg 841 acacgaggat tcttcatgaa aacacgcaaa cagataaggc tctgtataat cggctggttc 901 ctttagtgaa tggtgtcaga gaattttcag aaattcagct tgctcggcta aaaaaactgg 961 gaataaataa gactgatccg agcacactga cagaagagga agtgagtaaa tttgcccgtc 1021 tcgacatcga cccatctacc atcacgtggc agagagtatt ggatacaaat gaccgatttc 1081 tacgaaaaat aaccatcggg cagggaaaca cagagaaggg ccattaccgg caggcgcagt 1141 ttgacatcgc agtggccagc gagatcatgg cggtgctggc cctgacggac agcctcgcag 1201 acatgaaggc acggctggga aggatggtgg tggccagtga caaaagcggg cagcctgtga 1261 cagcagatga tttgggggtg acaggtgctt tgacagtttt gatgaaagat gcaataaaac 1321 caaacctgat gcagaccctg gaagggacac ctgtgttcgt gcatgcgggc ccttttgcta 1381 acattgctca cggcaactct tcagtgttgg ctgataaaat tgccctgaaa ctggttggtg 1441 aagaaggatt tgtagtgacc gaagctggct ttggtgctga catcggaatg gagaaattct 1501 tcaacatcaa gtgccgagct tccggcttgg tgcccaacgt ggttgtgtta gtggcaacgg 1561 tgcgagctct gaagatgcat ggaggcgggc caagtgtaac ggctggtgtt cctcttaaga 1621 aagaatatac agaggagaac atccagctgg tggcagacgg ctgctgtaac ctccagaagc 1681 aaattcagat cactcagctc tttggggttc ccgttgtggt ggctctgaat gtcttcaaga 1741 ccgacacccg cgctgagatt gacttggtgt gtgagcttgc aaagcgggct ggtgcctttg 1801 atgcagtccc cTGCTATCAC TGGTCGGTTG GTGGAAAAGG ATCGGTGGAC TTGGCTCGGG 1861 CTGTGAGAGA GGCTGCGAGT AAAAGAAGCC GATTCCAGTT CCTGTATGAT GTTCAGGTTC 1921 CAATTGTGGA CAAGATAAGG ACCATTGCTC AGGCTGTCTA TGGAGCCAAA GATATTGAAC 1981 TCTCTCCTGA GGCACAAGCC AAAATAGATC GTTACACTCA ACAGGGTTTT GGAAATTTGC 2041 CCATCTGCAT GGCAAAGACC CACCTTTCTC TATCTCACCA ACCTGACAAA AAAGGTGTGC 2101 CAAGGGACTT CATCTTACCT ATCAGTGACG TCCGGGCCAG CATAGGCGCT GGGTTCATTT 2161 ACCCTTTGGT CGGAACGATG AGCACCATGC CAGGACTGCC CACCCGGCCC TGCTTTTATG 2221 ACATAGATCT TGATACCGAA ACAGAACAAG TTAAAGGCTT GTTCTACCCA ACTTTCTTGT 2281 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2341 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2401 GAAAGGACGA TAAATGCATC CAACAAAAAC ATAAACGCGT TAAGTCgaca atcaacctct 2461 ggattacaaa atttgtgaaa gatt