Construct: ORF TRCN0000472697

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011439.1_s317c1
Derived from:
ccsbBroadEn_04106
DNA Barcode:
CAATACCTGCAAAGTACAACAGAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
VEPH1 (79674)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472697
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79674 VEPH1 ventricular zone expressed ... NM_001167912.2 100% 100%
2 human 79674 VEPH1 ventricular zone expressed ... NM_024621.2 100% 100%
3 human 79674 VEPH1 ventricular zone expressed ... XM_024453746.1 100% 100%
4 human 79674 VEPH1 ventricular zone expressed ... XM_024453747.1 100% 100%
5 human 79674 VEPH1 ventricular zone expressed ... XM_024453748.1 100% 100%
6 human 79674 VEPH1 ventricular zone expressed ... XM_011513134.2 94.7% 92.5% (many diffs)
7 human 79674 VEPH1 ventricular zone expressed ... NM_001167911.2 94.5% 94.5% 1873_1874ins135
8 human 79674 VEPH1 ventricular zone expressed ... XM_011513135.2 94.5% 94.4% 0_1ins131;3_3delGinsCTTCA
9 human 79674 VEPH1 ventricular zone expressed ... NM_001167915.2 23.6% 21.2% (many diffs)
10 human 79674 VEPH1 ventricular zone expressed ... NM_001167916.2 23.6% 21.2% (many diffs)
11 human 79674 VEPH1 ventricular zone expressed ... NM_001167917.1 21.2% 21.1% 530T>G;531_532ins1968
12 human 79674 VEPH1 ventricular zone expressed ... XM_024453749.1 21.2% 21.1% 530T>G;531_532ins1968
13 human 79674 VEPH1 ventricular zone expressed ... XM_024453750.1 21.2% 21.1% 530T>G;531_532ins1968
14 mouse 72789 Veph1 ventricular zone expressed ... NM_145820.3 85.3% 85.4% (many diffs)
15 mouse 72789 Veph1 ventricular zone expressed ... XM_006502142.3 81.9% 82.1% (many diffs)
16 mouse 72789 Veph1 ventricular zone expressed ... XM_006502143.3 75.4% 76.2% (many diffs)
17 mouse 72789 Veph1 ventricular zone expressed ... XM_011240244.2 19.7% 19.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2568
ORF length:
2499
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gcatcaactg ttcagactgg ttttgggaca aaaagatctt tcacgagctg
121gggacctctt ctccttagat gactctgaga ttgaagacag ccttacagaa gctttggagc
181aaattaagat aattagctca tcttcagatt accaaaccaa taacaatgac caggcagtag
241ttgaaatctg tatcacaaga atcacaacag ccatcagaga gaccgagtcc attgaaaagc
301atgcaaaggc ccttgtgggg ctctgggact cctgcttgga acataacctg agaccctttg
361ggaaagacga agacactcct catgcaaaaa tcgcatctga tatcatgagt tgcattttac
421agaattacaa ccgaccccca gtgatggcat tagccatccc cattgcagtg aaattcctcc
481acagaggcaa caaggaactg tgcaggaata tgtctaacta cctgtctctg gctgcaatta
541ccaaggcaga tctcctggct gatcacacgg aagttatagt aaagagcata ctccaaggta
601acaccatgtt gttgagagtg ttacctgctg tgtatgaaaa gcagcctcag ccaattaata
661gacacctgac agaactcctg gccttgatgt ctcagctgga acagccagaa cagtaccatc
721tactacggct tttgcatgta gcagcaaaga aaaaacaact cgaggtagtt cagaagtgta
781ttcctttcct aattgggcat ttgaaggatt caacccataa tgacatcatc ctaaacatcc
841tcatagagat agcagtctat gagccagtgg ctttgaacag ttttcttcca atgctgaaag
901agattggtga gagattcccc tacctcactg gacagatggc aaggatttat ggagctgttg
961ggcatgtgga tgaagagaga gccaggagct gcctgacata cctggtgagc caactggcca
1021acatggagca ttcgtttcac catattctcc tgctggagat taaaagcatc accgacacct
1081tctcctcaat cttgggccct cagagcagag acatcttccg catgagcaac agcttcaccg
1141ccattgctaa actccttacc cgacaactgg aaaataccaa ggctggaagt ggcaggagaa
1201aaatcagcac tgaaattgaa ttccctgaga aactggaaga aaccaagctc atagtaactg
1261aaaatgaaga ccatgaaaaa ctccaagtta aaatccaggc ttttgaagac aagataaatg
1321cagggagcaa tacccctggc tctatcagaa gatatagtct gggccaagtt tctaaagaag
1381aaagaaaaaa cattagattt aacaggtcaa aaagtttggc tttccacact atgctcacaa
1441agggtgtggg ttcagatgac ggcgaagatg aaaacagggg agacatacca gccagcatct
1501ctctttcaga aatagaccca cttggccaag gaaatgacaa gctgccgttt aagacagaca
1561ctgagagatc acagctgggg gagtcttcag tttcataccc aaatattata catatagact
1621cagagaattt gtcagaaact gttaaagaaa actcccagga agaaactcca gagacaactg
1681caagtcctat agaataccaa gataagctct acttgcactt aaaaaaaaac ctcagcaaag
1741tgaaagcata tgccatggaa attggaaaga agattccagt ccctgatcag tgtaccattg
1801aagacactgt gagaagttgt gtagcaaagt tgttcttcac ctgctccctg aagggtcatt
1861actgcctata cagtaagtcc agttttattc tcatcagcca agaacctcag ccatggatcc
1921agatcatgtt tctatttcag cagagcctgt ttcctgaacc cctgtccatt cagagtcatt
1981ctgtgcaatt cctcagagct ctgtgggaga agacccaggc agggggtgct cacagctttg
2041aaactgccat gatggagtcc acgtttccac agcagaagga tctggaccag gtacagctcc
2101atctggaaga agtgaggttc tttgacgtgt ttggcttcag tgaaacagca ggagcatGGC
2161AATGCTTCAT GTGCAACAAT CCTGAGAAAG CAACTGTTGT AAATCAAGAT GGCCAGCCTC
2221TCATAGAAGG AAAACTTAAA GAGAAGCAAG TCAGATGGAA GTTCATCAAA AGGTGGAAAA
2281CACGCTATTT TACACTGGCT GGAAATCAAC TTCTGTTTCA AAAAGGAAAG TCTAAAGATG
2341ACCCTGACGA CTGCCCAATA GAACTCAGCA AAGTACAGAG TGTGAAGGCT GTGGCCAAGA
2401AACGCAGGGA CCGCTCTCTC CCCCGGGCTT TCGAAATCTT CACAGACAAT AAAACCTATG
2461TCTTTAAGGC CAAGGATGAG AAGAATGCAG AAGAATGGCT CCAGTGCATC AACGTGGCAG
2521TTGCCCAAGC CAAAGAAAGG GAAAGTAGAG AAGTAACCAC ATATCTGTTG CCAACTTTCT
2581TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
2641CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
2701GTGGAAAGGA CGACAATACC TGCAAAGTAC AACAGATACG CGTTAAGTCg acaatcaacc
2761tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)