Construct: ORF TRCN0000472710

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005368.1_s317c1
Derived from:
ccsbBroadEn_07461
DNA Barcode:
GAATTCGTGAGATAATCGGGTTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDE4DIP (9659)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472710
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_022359.6 99.7% 99.3% 557T>C;738C>G
2 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_001195261.1 55% 52.4% (many diffs)
3 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_001002810.3 53.4% 52.2% (many diffs)
4 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_001195260.1 45.2% 44.8% 0_1ins507;50T>C;231C>G
5 human 653513 LOC653513 phosphodiesterase 4D intera... NR_037182.1 29.5% (many diffs)
6 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_001002812.2 15% 14.6% (many diffs)
7 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_011510176.2 13.3% 13.3% 557T>C;738C>G;931_6933del
8 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_024451068.1 13.2% 13.2% 557T>C;738C>G;931_6978del
9 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_024451067.1 12.8% 12.7% 557T>C;738C>G;931_7248del
10 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_001350521.2 12.5% 12.5% 557T>C;738C>G;931_7380del
11 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_011510175.2 12.4% 12.4% 557T>C;738C>G;931_7446del
12 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002878.2 12.4% 12.4% 557T>C;738C>G;931_7449del
13 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_011510173.2 12% 12% 557T>C;738C>G;931_7695del
14 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_011510172.2 12% 12% 557T>C;738C>G;931_7698del
15 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_001198832.2 10.2% 10% (many diffs)
16 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002903.2 10.2% 10% (many diffs)
17 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_001350523.1 10.1% 9.9% (many diffs)
18 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002899.2 9.9% 9.7% (many diffs)
19 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_001350522.1 9.8% 9.6% (many diffs)
20 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_006711646.2 9.2% 9% (many diffs)
21 human 100996724 LOC100996724 phosphodiesterase 4D intera... NR_144517.1 8.5% (many diffs)
22 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_011510179.1 6.7% 6.5% (many diffs)
23 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_014644.5 6.7% 6.5% (many diffs)
24 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_001198834.3 6.6% 6.4% (many diffs)
25 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_006711650.4 6.6% 6.3% (many diffs)
26 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_006711652.1 6.5% 6.3% (many diffs)
27 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_006711651.1 6.5% 6.3% (many diffs)
28 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002887.1 5.4% 5.4% (many diffs)
29 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002888.1 5.4% 5.4% (many diffs)
30 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002889.2 5.4% 5.4% (many diffs)
31 mouse 83679 Pde4dip phosphodiesterase 4D intera... NM_001289702.1 10.3% 9.7% (many diffs)
32 mouse 83679 Pde4dip phosphodiesterase 4D intera... NM_001039376.2 10.1% 9.6% (many diffs)
33 mouse 83679 Pde4dip phosphodiesterase 4D intera... NM_001289701.1 10.1% 9.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
996
ORF length:
930
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa gggcacagac agcgggtcct gctgccgccg ccgatgcgac tttggctgct
121gctgtcgcgc gtcccgccgg gctcactaca cgccttaccg gtccggggac gcgacgcgaa
181cccctcagtc cccacggcag accccgagcc gggaaagacg gcgcccggag ccagccggga
241gctgggcagc agcggccgag gaggaagaag cagctgcggc ggccacaccc tggatgagag
301attattttgc agaggatgat ggggagatgg tacccagaac gagtcacaca gcagcttttc
361ttagtgacac taaagatcga ggccctccag tgcagtcaca gatctggaga agtggtgaaa
421aggtcccgtt tgtgcagaca tattccttga gagcatttga gaaaccccct caggtacaga
481cccaggctct tcgagacttt gagaagcacc tcaatgacct gaagaaggag aacttcagcc
541tcaagctgcg catctacttc ctggaggagc gcatgcaaca gaagtatgag gccagccggg
601aggacatcTA CAAGCGGAAC ACTGAGCTGA AGGTTGAAGT GGAGAGCTTG AAACGAGAAC
661TCCAGGACAA GAAACAGCAT CTGGATAAAA CATGGGCTGA TGTGGAGAAT CTCAACAGTC
721AGAATGAAGC TGAGCTCCGA CGCCAGTTTG AGGAGCGACA GCAGGAGACG GAGCATGTTT
781ATGAGCTCTT GGAGAATAAG ATGCAGCTTC TGCAGGAGGA ATCCAGGCTA GCAAAGAATG
841AAGCTGCGCG GATGGCAGCT CTGGTGGAAG CAGAGAAGGA GTGTAACCTG GAGCTCTCAG
901AGAAACTGAA GGGAGTCACC AAAAACTGGG AAGATGTACC AGGAGACCAG GTCAAGCCCG
961ACCAATACAC TGAGGCCCTG GCCCAGAGGG ACAAGTACCC AACTTTCTTG TACAAAGTGG
1021TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1081CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1141AGAATTCGTG AGATAATCGG GTTTTACGCG TTAAGTCgac aatcaacctc tggattacaa
1201aatttgtgaa agatt

Download FASTA (ORF) (Full)