Construct: ORF TRCN0000472732
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016705.1_s317c1
- Derived from:
- ccsbBroadEn_13787
- DNA Barcode:
- ATTTTAATGCCCTACTTTCGGATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472732
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285676 | ZNF454 | zinc finger protein 454 | NM_001323307.2 | 26.6% | 1.7% | (many diffs) |
| 2 | human | 285676 | ZNF454 | zinc finger protein 454 | NM_001323308.2 | 26.6% | 1.7% | (many diffs) |
| 3 | human | 285676 | ZNF454 | zinc finger protein 454 | NM_001323309.2 | 26.6% | 1.7% | (many diffs) |
| 4 | human | 285676 | ZNF454 | zinc finger protein 454 | NM_001323310.2 | 26.6% | 1.7% | (many diffs) |
| 5 | human | 725 | C4BPB | complement component 4 bind... | XM_005273255.2 | 14.3% | 5.5% | (many diffs) |
| 6 | human | 387723 | C10orf143 | chromosome 10 open reading ... | NR_034125.1 | 6.5% | (many diffs) | |
| 7 | human | 114818278 | ZNF71-SMIM17 | ZNF71-SMIM17 readthrough (N... | NR_163263.1 | 6.2% | (many diffs) | |
| 8 | human | 114818278 | ZNF71-SMIM17 | ZNF71-SMIM17 readthrough (N... | NR_163262.1 | 5.9% | (many diffs) | |
| 9 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_001743501.1 | 5.8% | (many diffs) | |
| 10 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_001743500.1 | 5.6% | (many diffs) | |
| 11 | human | 55131 | RBM28 | RNA binding motif protein 28 | XR_001744830.1 | 1.8% | (many diffs) | |
| 12 | human | 79786 | KLHL36 | kelch like family member 36 | XR_933433.3 | 1.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 129
- ORF end:
- 330
- ORF length:
- 201
- Sequence:
-
1 TGCCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT 61 TTTTCTTCCA TTTCAGGTGT CGTGAGGCTA GCATCGATTG ATCAACAAGT TTGTACAAAA 121 AAGTTGGCAT GGCCTCCTCC CAAGTAGCTG GGATTACAGG TGCGTGCCAC CACGCTGGGC 181 CGATTTTTGT GTTTTCGGTA GAGACGGGGT TTCACTGTGT TGGCCAGGCT GGTCTTGAAC 241 TCCTGACCTC AAGTGATCCT CCCACCTTGG CCTCCCAAGG TGCTGGGATT GCAGGCGTGA 301 GCCACCGTGC CCGGCCTCAG AGTAGTTCTT ACCCAACTTT CTTGTACAAA GTGGTTGATA 361 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 421 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATTTT 481 AATGCCCTAC TTTCGGATAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 541 tgaaagatt