Construct: ORF TRCN0000472736
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013390.1_s317c1
- Derived from:
- ccsbBroadEn_14358
- DNA Barcode:
- ATTGGGATTGACGTTGCAACCCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FCHO2 (115548)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472736
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 115548 | FCHO2 | FCH and mu domain containin... | XM_017009023.2 | 79.8% | 72% | (many diffs) |
| 2 | human | 115548 | FCHO2 | FCH and mu domain containin... | XM_017009022.2 | 68.8% | 62% | (many diffs) |
| 3 | human | 115548 | FCHO2 | FCH and mu domain containin... | XM_017009020.2 | 65.5% | 59.1% | (many diffs) |
| 4 | human | 115548 | FCHO2 | FCH and mu domain containin... | XM_017009021.2 | 65.5% | 59.1% | (many diffs) |
| 5 | human | 115548 | FCHO2 | FCH and mu domain containin... | XM_017009019.2 | 61.1% | 55% | (many diffs) |
| 6 | human | 115548 | FCHO2 | FCH and mu domain containin... | XM_017009018.2 | 60.3% | 54.4% | (many diffs) |
| 7 | human | 115548 | FCHO2 | FCH and mu domain containin... | XM_017009017.2 | 59.9% | 54% | (many diffs) |
| 8 | human | 115548 | FCHO2 | FCH and mu domain containin... | NM_138782.3 | 42.3% | 38.1% | (many diffs) |
| 9 | human | 115548 | FCHO2 | FCH and mu domain containin... | NM_001146032.2 | 38.3% | 34.1% | (many diffs) |
| 10 | human | 115548 | FCHO2 | FCH and mu domain containin... | XM_017009016.2 | 38.1% | 33.9% | (many diffs) |
| 11 | human | 115548 | FCHO2 | FCH and mu domain containin... | XR_001741993.2 | 21.5% | (many diffs) | |
| 12 | mouse | 218503 | Fcho2 | FCH domain only 2 | NM_172591.3 | 39.8% | 36.7% | (many diffs) |
| 13 | mouse | 218503 | Fcho2 | FCH domain only 2 | XM_011244652.2 | 36.9% | 33.7% | (many diffs) |
| 14 | mouse | 218503 | Fcho2 | FCH domain only 2 | XR_001780779.1 | 18% | (many diffs) | |
| 15 | mouse | 218503 | Fcho2 | FCH domain only 2 | XR_873743.2 | 18% | (many diffs) | |
| 16 | mouse | 218503 | Fcho2 | FCH domain only 2 | XR_001780778.1 | 17.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1194
- ORF length:
- 1128
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtatttcatc gagaattttt ggggggaaaa aaatagtggc tttgatgtcc 121 tctaccataa tatgaaacat ggacagatat cgacaaaaga actagcagat tttgtaaggg 181 aacgagctac catagaggag gcatactcca ggtcaatgac aaaactagca aaatctgcaa 241 gcaattattc acaacttgga acatttgcac cagtatggga tgtattcaaa acatctacag 301 agaaattagc aaattgtcac ttggatcttg ttagaaaatt acaagaatta ataaaggaag 361 ttcagaagta tggagaagaa caagtaaagt ctcataaaaa gactaaagaa gaagttgcag 421 gaactctgga agctgtccaa accattcaga gcataactca ggccctccag aaatccaagg 481 aaaattacaa tgccaagtgt gtagaacagg agcgtttgaa aaaggaagga gctacacaaa 541 gagaaataga aaaggcagct gttaaatcta agaaagctac agatacttat aaactctatg 601 tGGAAAAATA TGCATTAGCA AAAGCTGATT TCGAACAGAA AATGACAGAA ACAGCTCAGA 661 AATTTCAAGA TATTGAAGAA ACTCATCTCA TTCACATAAA GGAAATTATA GGATCCTTGT 721 CAAATGCTAT AAAGGAAATT CATTTGCAGA TAGGCCAGGT CCATGAAGAA TTTATAAATA 781 ACATGGCTAA TACTACAGTT GAAAGTTTGA TACAAAAATT TGCTGAGTCA AAAGGCACTG 841 GGAAGGAAAG ACCTGGCCTC ATTGAATTTG AAGAATGTGA CACTGCTAGT GCAGTTGAAG 901 GTATAAAACC AAGGAAAAGA AAGACCTTTG CTTTGCCAGG AATCATTAAA AAGGAAAAAG 961 ATGCAGAATC TGTATGGAGT TTCACTGTTG TTGCCCAGGT TGGAATGCAA TGGCGCGATC 1021 TTGGCTTACT GCATTCTCCA CCTCCCAGGT TCAAGCGATT CTCCTCCTAC CTCAGCCTCC 1081 CAAGTAGCTG GAATTACGGC GCCCACCACC ACATCTGGCT AATTTTTTGT ATTTTTAGTA 1141 GAGACAGGGT TTCACCATAT TGGCCAGGCT GGTCGCGAAC TCCTGACCTC AGGTGCCCAA 1201 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1261 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1321 TATCTTGTGG AAAGGACGAA TTGGGATTGA CGTTGCAACC CCGACGCGTT AAGTCgacaa 1381 tcaacctctg gattacaaaa tttgtgaaag att