Construct: ORF TRCN0000472759
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016635.1_s317c1
- Derived from:
- ccsbBroadEn_14157
- DNA Barcode:
- ACGGCCTATTAGACCTGAAACGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TM6SF1 (53346)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472759
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | NM_001330385.3 | 76.7% | 4.7% | (many diffs) |
| 2 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XM_017022329.2 | 76.7% | 4.7% | (many diffs) |
| 3 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XM_017022330.1 | 76.7% | 4.7% | (many diffs) |
| 4 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XM_017022331.2 | 76.7% | 4.7% | (many diffs) |
| 5 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XR_931848.2 | 56.6% | (many diffs) | |
| 6 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XR_001751320.1 | 46.2% | (many diffs) | |
| 7 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XM_017022322.1 | 45.5% | 2.8% | (many diffs) |
| 8 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | NM_001144903.3 | 41.9% | 2.6% | (many diffs) |
| 9 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | NM_001353878.2 | 39.4% | 2.5% | (many diffs) |
| 10 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XM_017022321.1 | 39.4% | 2.4% | (many diffs) |
| 11 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | NM_023003.5 | 38.3% | 2.4% | (many diffs) |
| 12 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XM_011521679.2 | 38% | 2.4% | (many diffs) |
| 13 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XM_017022320.1 | 36.3% | 2.2% | (many diffs) |
| 14 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XR_001751318.1 | 35.9% | (many diffs) | |
| 15 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | NR_148561.2 | 24.9% | (many diffs) | |
| 16 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | NR_148560.2 | 24.1% | (many diffs) | |
| 17 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XR_001751319.2 | 14.1% | (many diffs) | |
| 18 | mouse | 107769 | Tm6sf1 | transmembrane 6 superfamily... | NM_145375.4 | 34.9% | 2.4% | (many diffs) |
| 19 | mouse | 107769 | Tm6sf1 | transmembrane 6 superfamily... | NR_111910.1 | 19.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 171
- ORF length:
- 105
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGAG TGCCTCGCGG CCACCGGGGT CTTCGTGCTG TCCCTCTCGG CCATCCCGGT 121 CACCTATGTC TTCAACCACC TGGCGGCCCA GCATGATTCC TGGACTATTG TAGGGGTTGC 181 TGCCCTCATC CTGTTCCTGG TAGCACTGCT GGCTCGTGTC CTCGTCAAAA GAAAACCACC 241 CCGGGACCCA CTGTTCTATG TGTATGCAGT TTTTGGATTT ACCAGCGTGG TGAACCTCAT 301 CATAGGACTG GAGCAAGATG GAATCATTGA CGGGTTCATG ACACACTACT TGAGAGAGGG 361 TGAACCGTAT CTGAACACCG CATATGGGCA CATGATCTGC TACTGGGATG GCTCTGCTCA 421 TTATCTGATG TACCTGGTGA TGGTGGCAGC CATAGCATGG GAATGCTGGC ATATATGTTT 481 TATTCTGTTC CTTACTTTGT GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC 541 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA 601 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACGGC CTATTAGACC 661 TGAAACGTCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt