Transcript: Human XM_017022331.2

PREDICTED: Homo sapiens transmembrane 6 superfamily member 1 (TM6SF1), transcript variant X12, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TM6SF1 (53346)
Length:
720
CDS:
95..661

Additional Resources:

NCBI RefSeq record:
XM_017022331.2
NBCI Gene record:
TM6SF1 (53346)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017022331.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000138288 CCGTATCTGAACACCGCATAT pLKO.1 395 CDS 100% 10.800 15.120 N TM6SF1 n/a
2 TRCN0000135885 GCTCTGCTCATTATCTGATGT pLKO.1 441 CDS 100% 4.950 3.465 N TM6SF1 n/a
3 TRCN0000137625 GCTTAGTGGTTCCTGGATGTT pLKO.1 628 CDS 100% 4.950 3.465 N TM6SF1 n/a
4 TRCN0000135809 CCACTGTTCTATGTGTATGCA pLKO.1 278 CDS 100% 3.000 2.100 N TM6SF1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017022331.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12020 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_12020 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000480894 TTGCCTACGGTTGCTGGAAAACCC pLX_317 62.5% 100% 100% V5 n/a
4 ccsbBroadEn_14157 pDONR223 100% 76.7% 4.7% None (many diffs) n/a
5 ccsbBroad304_14157 pLX_304 0% 76.7% 4.7% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000472759 ACGGCCTATTAGACCTGAAACGTC pLX_317 100% 76.7% 4.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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