Construct: ORF TRCN0000472785
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019029.3_s317c1
- Derived from:
- ccsbBroadEn_14680
- DNA Barcode:
- CCCCCACGAGTCAAACATTTAGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- JAK2 (3717)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472785
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3717 | JAK2 | Janus kinase 2 | NM_001322194.1 | 99.9% | 100% | 3396_3397insTGA |
| 2 | human | 3717 | JAK2 | Janus kinase 2 | NM_001322195.1 | 99.9% | 100% | 3396_3397insTGA |
| 3 | human | 3717 | JAK2 | Janus kinase 2 | NM_001322196.1 | 99.9% | 100% | 3396_3397insTGA |
| 4 | human | 3717 | JAK2 | Janus kinase 2 | NM_004972.3 | 99.9% | 100% | 3396_3397insTGA |
| 5 | human | 3717 | JAK2 | Janus kinase 2 | NM_001322204.1 | 86.7% | 86.8% | 0_1ins447;2949_2950insTGA |
| 6 | human | 3717 | JAK2 | Janus kinase 2 | NM_001322198.1 | 64.1% | 64.2% | 0_1ins1215;2181_2182insTGA |
| 7 | human | 3717 | JAK2 | Janus kinase 2 | NM_001322199.1 | 64.1% | 64.2% | 0_1ins1215;2181_2182insTGA |
| 8 | mouse | 16452 | Jak2 | Janus kinase 2 | NM_001048177.2 | 88.5% | 93.7% | (many diffs) |
| 9 | mouse | 16452 | Jak2 | Janus kinase 2 | NM_008413.3 | 88.5% | 93.7% | (many diffs) |
| 10 | mouse | 16452 | Jak2 | Janus kinase 2 | XM_011247155.2 | 88.5% | 93.7% | (many diffs) |
| 11 | mouse | 16452 | Jak2 | Janus kinase 2 | XM_006526713.3 | 85% | 89.8% | (many diffs) |
| 12 | mouse | 16452 | Jak2 | Janus kinase 2 | XM_006526714.3 | 73.4% | 78% | (many diffs) |
| 13 | mouse | 16452 | Jak2 | Janus kinase 2 | XM_017318068.1 | 57% | 61.2% | (many diffs) |
| 14 | mouse | 16452 | Jak2 | Janus kinase 2 | XM_006526715.3 | 46.5% | 48.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3465
- ORF length:
- 3396
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggaatggcc tgccttacga tgacagaaat ggagggaaca tccacctctt 121 ctatatatca gaatggtgat atttctggaa atgccaattc tatgaagcaa atagatccag 181 ttcttcaggt gtatctttac cattcccttg ggaaatctga ggcagattat ctgacctttc 241 catctgggga gtatgttgca gaagaaatct gtattgctgc ttctaaagct tgtggtatca 301 cacctgtgta tcataatatg tttgctttaa tgagtgaaac agaaaggatc tggtatccac 361 ccaaccatgt cttccatata gatgagtcaa ccaggcataa tgtactctac agaataagat 421 tttactttcc tcgttggtat tgcagtggca gcaacagagc ctatcggcat ggaatatctc 481 gaggtgctga agctcctctt cttgatgact ttgtcatgtc ttacctcttt gctcagtggc 541 ggcatgattt tgtgcacgga tggataaaag tacctgtgac tcatgaaaca caggaagaat 601 gtcttgggat ggcagtgtta gatatgatga gaatagccaa agaaaacgat caaaccccac 661 tggccatcta taactctatc agctacaaga cattcttacc aaaatgtatt cgagcaaaga 721 tccaagacta tcatattttg acaaggaagc gaataaggta cagatttcgc agatttattc 781 agcaattcag ccaatgcaaa gccactgcca gaaacttgaa acttaagtat cttataaatc 841 tggaaactct gcagtctgcc ttctacacag agaaatttga agtaaaagaa cctggaagtg 901 gtccttcagg tgaggagatt tttgcaacca ttataataac tggaaacggt ggaattcagt 961 ggtcaagagg gaaacataaa gaaagtgaga cactgacaga acaggattta cagttatatt 1021 gcgattttcc taatattatt gatgtcagta ttaagcaagc aaaccaagag ggttcaaatg 1081 aaagccgagt tgtaactatc cataagcaag atggtaaaaa tctggaaatt gaacttagct 1141 cattaaggga agctttgtct ttcgtgtcat taattgatgg atattataga ttaactgcag 1201 atgcacatca ttacctctgt aaagaagtag cacctccagc cgtgcttgaa aatatacaaa 1261 gcaactgtca tggcccaatt tcgatggatt ttgccattag taaactgaag aaagcaggta 1321 atcagactgg actgtatgta cttcgatgca gtcctaagga ctttaataaa tattttttga 1381 cttttgctgt cgagcgagaa aatgtcattg aatataaaca ctgtttgatt acaaaaaatg 1441 agaatgaaga gtacaacctc agtgggacaa agaagaactt cagcagtctt aaagatcttt 1501 tgaattgtta ccagatggaa actgttcgct cagacaatat aattttccag tttactaaat 1561 gctgtccccc aaagccaaaa gataaatcaa accttctagt cttcagaacg aatggtgttt 1621 ctgatgtacc aacctcacca acattacaga ggcctactca tatgaaccaa atggtgtttc 1681 acaaaatcag aaatgaagat ttgatattta atgaaagcct tggccaaggc acttttacaa 1741 agatttttaa aggcgtacga agagaagtag gagactacgg tcaactgcat gaaacagaag 1801 ttcttttaaa agttctggat aaagcacaca gaaactattc agagtctttc tttgaagcag 1861 caagtatgat gagcaagctt tctcacaagc atttggtttt aaattatgga gtatgtgtct 1921 gtggagacga gaatattctg gttcaggagt ttgtaaaatt tggatcacta gatacatatc 1981 tgaaaaagaa taaaaattgt ataaatatat tatggaaact tgaagttgct aaacagttgg 2041 catgggccat gcattttcta gaagaaaaca cccttattca tgggaatgta tgtgccaaaa 2101 atattctgct tatcagagaa gaagacagga agacaggaaa tcctcctttc atcaaactta 2161 gtgatcctgg cattagtatt acagttttgc caaaggacat tcttcaggag agaataccat 2221 gggtaccacc tgaatgcatt gaaaatccta aaaatttaaa tttggcaaca gacaaatgga 2281 gttttggtac cactttgtgg gaaatctgca gtggaggaga taaacctcta agtgctctgg 2341 attctcaaag aaagctacaa ttttatgaag ataggcatca gcttcctgca ccaaagtggg 2401 cagaattagc aaaccttata aataattgta tggattatga accagatttc aggccttctt 2461 tcagagccat catacgagat cttaacagtt tgtttactcc agattatgaa ctattaacag 2521 aaaatgacat gttaccaaat atgaggatag gtgccctggg gttttctggt gcctttgaag 2581 accgggatcc tacacagttt gaagagagac atttgaaatt tctacagcaa cttggcaagg 2641 gtaattttgg gagtgtggag atgtgccggt atgaccctct acaggacaac actggggagg 2701 tggtcgctgt aaaaaagctt cagcatagta ctgaagagca cctaagagac tttgaaaggg 2761 aaattgaaat cctgaaatcc ctacagcatg acaacattgt aaagtacaag ggagtgtgct 2821 acagtgctgg tcggcgtaat ctaaaattaa ttatggaata tttaccatat ggaagtttac 2881 gagactatct tcaaaaacat aaagaacgga tagatcacat aaaacttctg cagtacacat 2941 ctcagatatg caagggtatg gagtatcttg gtacaaaaag gtatatccac agggatctgg 3001 caacgagaaa tatattggtg gagaacgaga acagagttaa aattGGAGAT TTTGGGTTAA 3061 CCAAAGTCTT GCCACAAGAC AAAGAATACT ATAAAGTAAA AGAACCTGGT GAAAGTCCCA 3121 TATTCTGGTA TGCTCCAGAA TCACTGACAG AGAGCAAGTT TTCTGTGGCC TCAGATGTTT 3181 GGAGCTTTGG AGTGGTTCTG TATGAACTTT TCACATACAT TGAGAAGAGT AAAAGTCCAC 3241 CAGCGGAATT TATGCGTATG ATTGGCAATG ACAAACAAGG ACAGATGATC GTGTTCCATT 3301 TGATAGAACT TTTGAAGAAT AATGGAAGAT TACCAAGACC AGATGGATGC CCAGATGAGA 3361 TCTATATGAT CATGACAGAA TGCTGGAACA ATAATGTAAA TCAACGCCCC TCCTTTAGGG 3421 ATCTAGCTCT TCGAGTGGAT CAAATAAGGG ATAACATGGC TGGATGATGC CCAACTTTCT 3481 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 3541 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 3601 GTGGAAAGGA CGACCCCCAC GAGTCAAACA TTTAGTCACG CGTTAAGTCg acaatcaacc 3661 tctggattac aaaatttgtg aaagatt